Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	125,152	A→G	M252V (ATG→GTG)	lpdA →	dihydrolipoyl dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	125,152	0	A	G	100.0%	217.8 / NA	59	M252V (ATG→GTG)	lpdA	dihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/32);  total (27/32)

TTCGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGATGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCG  >  NZ_CP009273/125017‑125287                                                                                                                                        |                                                                                                                                        ttCGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTg                                                                                                                                       >  1:713783/1‑138 (MQ=255)   cGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTgg                                                                                                                                      >  2:772328/1‑137 (MQ=255)   cGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTgg                                                                                                                                      >  1:282745/1‑137 (MQ=255)              tCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGc                                                                                                                         <  2:210313/139‑1 (MQ=255)               cccGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCa                                                                                                                        >  1:954078/1‑139 (MQ=255)                     aGCTGACAAAGCCATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCt                                                                                                                  <  1:1165613/139‑1 (MQ=255)                        tGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGaa                                                                                                               >  1:45246/1‑139 (MQ=255)                           cAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCg                                                                                                            <  2:1276457/139‑1 (MQ=255)                           cAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCg                                                                                                            <  2:371970/139‑1 (MQ=255)                           cAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCg                                                                                                            <  2:45246/139‑1 (MQ=255)                            aaaGACATCGTTAAAGTCTTCACCAAGCGTTTCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGc                                                                                                           <  2:457581/139‑1 (MQ=255)                            aaaGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGc                                                                                                           <  1:772328/139‑1 (MQ=255)                                aCATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCg                                                                                                       <  1:805581/139‑1 (MQ=255)                                  aTCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGt                                                                                                      >  2:246654/1‑138 (MQ=255)                                  aTCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGt                                                                                                      >  2:803846/1‑138 (MQ=255)                                    cGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAgg                                                                                                                                  >  2:919395/1‑108 (MQ=255)                                    cGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAgg                                                                                                                                  <  1:919395/108‑1 (MQ=255)                                          aGTCTTCACCAAGCGTATCAGCAAGAAATTAAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGcc                                                                                             <  2:1197784/139‑1 (MQ=255)                                              ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGc                                                                                         >  1:579820/1‑139 (MQ=255)                                              ttCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGc                                                                                         >  2:392534/1‑139 (MQ=255)                                                cACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTg                                                                                          <  1:37272/136‑1 (MQ=255)                                                cACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTg                                                                                          <  1:873835/136‑1 (MQ=255)                                                cACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTg                                                                                          <  2:954078/136‑1 (MQ=255)                                                 aCCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTgg                                                                                      <  1:787191/139‑1 (MQ=255)                                                      gCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGc                                                                                  >  1:356416/1‑138 (MQ=255)                                                      gCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGc                                                                                  >  2:386359/1‑138 (MQ=255)                                                        gTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGAt                                                                               <  2:282745/139‑1 (MQ=255)                                                          aTCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTg                                                                             >  2:996282/1‑139 (MQ=255)                                                             aGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTc                                                                          >  1:913525/1‑139 (MQ=255)                                                             aGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTc                                                                          <  1:803846/139‑1 (MQ=255)                                                             aGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTc                                                                          <  1:707608/139‑1 (MQ=255)                                                               cAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCgt                                                                        >  1:350510/1‑139 (MQ=255)                                                                 aGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTa                                                                                    <  1:253110/125‑1 (MQ=255)                                                                 aGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTa                                                                                    >  2:946499/1‑125 (MQ=255)                                                                 aGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTa                                                                                    >  2:253110/1‑125 (MQ=255)                                                                 aGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTa                                                                                    <  1:946499/125‑1 (MQ=255)                                                                        cAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGaa                                                                <  2:643660/138‑1 (MQ=255)                                                                        cAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGaa                                                                <  2:1190405/138‑1 (MQ=255)                                                                          aCCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTgg                                                                                      <  2:608725/114‑1 (MQ=255)                                                                          aCCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTgg                                                                                      >  1:608725/1‑114 (MQ=255)                                                                           ccTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAg                                                                                                         <  2:555118/94‑1 (MQ=255)                                                                           ccTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAg                                                                                                         >  1:555118/1‑94 (MQ=255)                                                                              gATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGc                                                                                              >  1:1263288/1‑102 (MQ=255)                                                                              gATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGc                                                                                              <  2:1263288/102‑1 (MQ=255)                                                                                     gAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAg                                                                                                         <  1:420380/84‑1 (MQ=255)                                                                                     gAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAg                                                                                                         >  2:420380/1‑84 (MQ=255)                                                                                     gAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTc                                                   >  2:29511/1‑138 (MQ=255)                                                                                      aaaCCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCg                                                                                            <  2:2473/96‑1 (MQ=255)                                                                                      aaaCCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCg                                                                                            >  1:2473/1‑96 (MQ=255)                                                                                            aaGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAgg                                           <  2:194979/139‑1 (MQ=255)                                                                                                       gTTGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCg                                <  2:1206793/139‑1 (MQ=255)                                                                                                        ttGAAGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGt                               <  1:246654/139‑1 (MQ=255)                                                                                                           aaGCGAAAGAAGACGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTgg                             <  1:996282/138‑1 (MQ=255)                                                                                                                  agaagaCGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTgac                     <  1:936092/139‑1 (MQ=255)                                                                                                                       aCGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGAc                                                <  2:348455/107‑1 (MQ=255)                                                                                                                       aCGGCATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGAc                                                >  1:348455/1‑107 (MQ=255)                                                                                                                           cATTTATGTGACGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGt            >  1:1090137/1‑139 (MQ=255)                                                                                                                                     aCGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCcgcg  >  1:202167/1‑139 (MQ=255)                                                                                                                                     aCGGTGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCcgcg  >  1:1132453/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCGACCAGGTTATCCCGGCAGCTGACAAAGACATCGTTAAAGTCTTCACCAAGCGTATCAGCAAGAAATTCAACCTGATGCTGGAAACCAAAGTTACCGCCGTTGAAGCGAAAGAAGACGGCATTTATGTGACGATGGAAGGCAAAAAAGCACCCGCTGAACCGCAGCGTTACGACGCCGTGCTGGTAGCGATTGGTCGTGTGCCGAACGGTAAAAACCTCGACGCAGGCAAAGCAGGCGTGGAAGTTGACGACCGTGGTTTCATCCGCG  >  NZ_CP009273/125017‑125287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑