| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,142,301 | A→G | T383A (ACG→GCG) | yegD → | molecular chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,142,301 | 0 | A | G | 96.7% | 214.5 / ‑1.6 | 61 | T383A (ACG→GCG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (28/31); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.41e-01 | |||||||||||
GTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGACGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGCAGAACAGTTGCCGGGCATTC > NZ_CP009273/2142166‑2142433 | gTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcg < 1:1226380/138‑1 (MQ=255) tGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcgcg < 1:914223/139‑1 (MQ=255) gTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGAt < 1:342977/139‑1 (MQ=255) gcgcAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTc < 1:344478/138‑1 (MQ=255) cAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGa < 2:492618/139‑1 (MQ=255) agaagaGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGaa > 2:115180/1‑139 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcgcg > 1:1217590/1‑122 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcgcg < 2:1217590/122‑1 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcg > 1:1169570/1‑120 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcg < 2:1169570/120‑1 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTg > 2:355840/1‑138 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTg > 1:129910/1‑138 (MQ=255) tGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGACGCGGATTCTGGAACAGGTg > 1:192855/1‑138 (MQ=255) gCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGc > 2:149738/1‑138 (MQ=255) aaaaTTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAAc < 1:115180/139‑1 (MQ=255) aaaaTTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAAc < 1:355840/139‑1 (MQ=255) aaaaTTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAAc < 2:618666/139‑1 (MQ=255) tGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGcc > 2:379934/1‑139 (MQ=255) gCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGcc < 1:1127667/138‑1 (MQ=255) gCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAgg < 1:379934/139‑1 (MQ=255) cGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAgg < 2:54310/111‑1 (MQ=255) cGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAgg > 1:54310/1‑111 (MQ=255) cGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAAc < 1:594637/117‑1 (MQ=255) cGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAAc > 2:594637/1‑117 (MQ=255) cGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAgg > 2:983205/1‑138 (MQ=255) cgcgCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCgg < 2:753729/139‑1 (MQ=255) gcgcCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGa < 2:129910/139‑1 (MQ=255) gcgcCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGa < 2:284253/139‑1 (MQ=255) cTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGtt < 1:983205/139‑1 (MQ=255) aCTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATc > 2:616952/1‑139 (MQ=255) aCTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGACGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATc > 2:1269062/1‑139 (MQ=255) cTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGccc > 2:1109408/1‑118 (MQ=255) cTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGccc < 1:1109408/118‑1 (MQ=255) tttATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCaa < 2:966570/93‑1 (MQ=255) tttATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCaa > 1:966570/1‑93 (MQ=255) tttATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGa > 1:942338/1‑139 (MQ=255) tttATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATATACCTGa > 2:318183/1‑139 (MQ=255) gCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcgcg > 1:199298/1‑56 (MQ=255) gCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGcgcg < 2:199298/56‑1 (MQ=255) gCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGtg > 1:351968/1‑138 (MQ=255) cGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTg > 2:180777/1‑116 (MQ=255) cGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTg < 1:180777/116‑1 (MQ=255) gCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCg < 1:616952/139‑1 (MQ=255) ttAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGGTCTCCGCTGAt > 2:240467/1‑139 (MQ=255) tAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCgg < 1:801595/95‑1 (MQ=255) tAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCgg > 2:801595/1‑95 (MQ=255) ccAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGa > 1:453666/1‑93 (MQ=255) ccAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGa < 2:453666/93‑1 (MQ=255) cGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAg > 1:748951/1‑139 (MQ=255) ggCTGGAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCt < 2:24412/139‑1 (MQ=255) gAAAGCGCCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGc < 2:1066204/137‑1 (MQ=255) cgcCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGAt > 1:1061024/1‑55 (MQ=255) cgcCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGAt < 2:1061024/55‑1 (MQ=255) cgcCCTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGCAGAACAg > 1:869449/1‑139 (MQ=255) cgcCCTCAGCCAGCCACTGGCGCGGATTATGGTACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTCATTAAAAAAGCGCTGGCAGAAAAg > 2:175558/1‑139 (MQ=255) cccTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATa > 1:893114/1‑54 (MQ=255) cccTCAGCCAGCCACTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATa < 2:893114/54‑1 (MQ=255) gccagccaCTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGCAGAACAGTTGCCggg < 2:323395/139‑1 (MQ=255) ccaCTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAAcc > 2:1152295/1‑59 (MQ=255) ccaCTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAAcc < 1:1152295/59‑1 (MQ=255) ccaCTGGCGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGCAGAACAGTTGCCGGGCATTc > 2:999608/1‑139 (MQ=255) | GTGCGTAGCGCAGAAGAGTGCAAAATTGCTCTTTCAAGCGTAGCGGAAACCCGCGCCTCACTGCCGTTTATCAGCAATGAACTGGCTACGCTGATTAGCCAGCGAGGGCTGGAAAGCGCCCTCAGCCAGCCACTGACGCGGATTCTGGAACAGGTGCAACTGGCCCTGGATAACGCCCAGGAAAAACCGGACGTTATCTACCTGACCGGCGGTAGCGCCCGTTCTCCGCTGATTAAAAAAGCGCTGGCAGAACAGTTGCCGGGCATTC > NZ_CP009273/2142166‑2142433 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |