Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,176,075	A→G	I62I (ATT→ATC)	yegW ←	GntR family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,176,075	0	A	G	100.0%	183.1 / NA	49	I62I (ATT→ATC)	yegW	GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/26);  total (23/26)

CCACCTGCTGAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTAATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGC  >  NZ_CP009273/2175945‑2176209                                                                                                                                   |                                                                                                                                       ccaccTGCTGAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGcgcg                                                                                                                                 <  2:1054622/138‑1 (MQ=255)       gctgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGaaa                                                                                                                              <  2:145386/135‑1 (MQ=255)       gctgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGaaa                                                                                                                              >  1:145386/1‑135 (MQ=255)       gctgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGcc                                                                                                                          >  2:33894/1‑139 (MQ=255)       gctgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGcc                                                                                                                          >  1:304477/1‑139 (MQ=255)         tgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCgg                                                                                                                        >  2:185654/1‑139 (MQ=255)         tgAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCgg                                                                                                                        >  1:310901/1‑139 (MQ=255)           agaAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGt                                                                                                                       >  2:762979/1‑138 (MQ=255)             aaaaaCCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACGCCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTaa                                                                                                                    <  1:268363/139‑1 (MQ=255)             aaaaaCCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTaa                                                                                                                    <  1:295196/139‑1 (MQ=255)             aaaaaCCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTaa                                                                                                                    <  1:945459/139‑1 (MQ=255)                       cgcTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTaaa                                                                                                          >  1:1282651/1‑139 (MQ=255)                                 aaCGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCa                                                                                                 <  2:1282651/138‑1 (MQ=255)                                      gTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTaa                                                                                                                    <  1:85559/114‑1 (MQ=255)                                      gTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTaa                                                                                                                    >  2:85559/1‑114 (MQ=255)                                      gTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCggg                                                                                            >  1:110433/1‑138 (MQ=255)                                       tATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGaaa                                                                                                                              >  1:758591/1‑103 (MQ=255)                                       tATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGaaa                                                                                                                              <  2:758591/103‑1 (MQ=255)                                         ttCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCaa                                                                                         >  2:652379/1‑138 (MQ=255)                                            gAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAata                                                                                     <  1:762979/139‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  2:21705/138‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  2:207214/138‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  2:310901/138‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  1:1059497/138‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  1:652379/138‑1 (MQ=255)                                                   ttgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  1:52120/138‑1 (MQ=255)                                                    tgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               <  2:1101348/137‑1 (MQ=255)                                                    tgttgATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCa                                                                               >  1:1101348/1‑137 (MQ=255)                                                             gCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCCCCAGCAc                                                                    >  1:1203415/1‑139 (MQ=255)                                                                tGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCa                                                                                                       <  1:807732/101‑1 (MQ=255)                                                                tGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCa                                                                                                       >  2:807732/1‑101 (MQ=255)                                                                     tAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTgc                                                            >  1:626243/1‑139 (MQ=255)                                                                             cgAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCa                                                    >  1:1016212/1‑139 (MQ=255)                                                                                              ccTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGc                                   <  2:4329/139‑1 (MQ=255)                                                                                              ccTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGc                                   <  1:149349/139‑1 (MQ=255)                                                                                                tcttctTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCa                                  >  2:160837/1‑138 (MQ=255)                                                                                                tcttctTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCa                                  >  1:435833/1‑138 (MQ=255)                                                                                                tcttctTCCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCa                                  >  1:403174/1‑138 (MQ=255)                                                                                                  ttcttcCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCaa                                 <  2:656049/137‑1 (MQ=255)                                                                                                    cttcCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACt                              <  2:626243/138‑1 (MQ=255)                                                                                                     ttcCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACt                                                                                                                  <  2:726754/53‑1 (MQ=255)                                                                                                     ttcCAGCGCCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACt                                                                                                                  >  1:726754/1‑53 (MQ=255)                                                                                                            gcCTGCATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACTTAATATaaa                     <  2:435833/139‑1 (MQ=255)                                                                                                                 cATCGCCTTGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACTTAATATAAAgcggc                <  2:780928/139‑1 (MQ=255)                                                                                                                        ttGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCa                                                                                                 <  2:1000499/51‑1 (MQ=255)                                                                                                                        ttGCGCACCGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCa                                                                                                 >  1:1000499/1‑51 (MQ=255)                                                                                                                                cGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGc                                   <  2:1011246/105‑1 (MQ=255)                                                                                                                                cGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGc                                   >  1:1011246/1‑105 (MQ=255)                                                                                                                                cGTGATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCcgccgc  >  1:748952/1‑138 (MQ=255)                                                                                                                                   |                                                                                                                                       CCACCTGCTGAGAAAAACCTCGCGCTTCTTTCAACGAGTATTCGAAGATGTTGTTGATCTGCGTGCCGTAACCGCGCGAACGCGTCACCACACCCTCTTCTTCCAGCGCCTGCATCGCCTTGCGCACCGTAATGCGCGAAACGCCGGTTAACTGACTTAAATCACGCTCACCGGGCAAAATATTGCCATGCTCCAGCACCCCGCTGCGCACGGCATTTTTTACCGTTTCGGCAAACTTAATATAAAGCGGCGTGTTATCCGCCGC  >  NZ_CP009273/2175945‑2176209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑