Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,190,509	T→C	pseudogene (557/3796 nt)	BW25113_RS25680 →	WGR and DUF4132 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,190,509	0	T	C	100.0%	333.7 / NA	88	pseudogene (557/3796 nt)	BW25113_RS25680	WGR and DUF4132 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/46);  total (42/46)

TTCCTGTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGATCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAGGCGGT  >  NZ_CP009273/2190374‑2190642                                                                                                                                        |                                                                                                                                      ttCCTGTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGAccag                                                                                                                                    >  1:744357/1‑139 (MQ=255)   ccTGTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGAccagt                                                                                                                                   >  2:926275/1‑138 (MQ=255)   ccTGTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGAccagt                                                                                                                                   >  1:945615/1‑138 (MQ=255)      gTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGAccagt                                                                                                                                   >  1:875699/1‑135 (MQ=255)          agcaagcaGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGa                                                                                                                           <  2:875699/139‑1 (MQ=255)           gcaagcaGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGAc                                                                                                                          >  2:494749/1‑139 (MQ=255)              agcaGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGc                                                                                                                       <  2:832983/139‑1 (MQ=255)                        ttGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAaacaa                                                                                                             <  2:945615/139‑1 (MQ=255)                        ttGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAaacaa                                                                                                             <  2:259920/139‑1 (MQ=255)                           ccccAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAacaaca                                                                                                           <  2:452154/138‑1 (MQ=255)                                   aCGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAAAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTAcc                                                                                                   <  1:292306/138‑1 (MQ=255)                                      tCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCa                                                                                               >  1:668259/1‑139 (MQ=255)                                      tCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCa                                                                                               <  1:594343/139‑1 (MQ=255)                                        gCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCAc                                                                                              <  2:992761/138‑1 (MQ=255)                                         cTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCAc                                                                                              >  1:1015725/1‑137 (MQ=255)                                         cTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCAc                                                                                              >  1:740697/1‑137 (MQ=255)                                         cTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCAc                                                                                              >  1:740657/1‑137 (MQ=255)                                            aGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGt                                                                                          <  2:1015725/138‑1 (MQ=255)                                             gCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGGAAACAACACAGTTACCATCACCAGTaa                                                                                        <  2:54535/139‑1 (MQ=255)                                             gCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        <  1:390699/139‑1 (MQ=255)                                             gCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        <  1:724498/139‑1 (MQ=255)                                                 gTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGc                                                                                    >  2:201465/1‑139 (MQ=255)                                                  tAGTGTTTCGCAACGGGATAATAAAACAGCAACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCt                                                                                   >  2:405675/1‑139 (MQ=255)                                                  tAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCAt                                                                                                 <  1:535915/125‑1 (MQ=255)                                                  tAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCAt                                                                                                 >  2:535915/1‑125 (MQ=255)                                                  tAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCt                                                                                   >  1:580690/1‑139 (MQ=255)                                                  tAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCt                                                                                   <  2:580690/139‑1 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGc                                                                                                                       >  2:1153007/1‑98 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGc                                                                                                                       <  1:1153007/98‑1 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        >  1:313652/1‑129 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        <  2:313652/129‑1 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  2:705608/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  2:212220/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  1:206991/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  1:595457/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  2:93585/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  2:1085782/1‑139 (MQ=255)                                                       tgtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  2:1007937/1‑139 (MQ=255)                                                        gtCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACt                                                                              >  1:1081030/1‑138 (MQ=255)                                                         tCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCa                                                                            >  2:183321/1‑139 (MQ=255)                                                         tCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCa                                                                            <  2:1136623/139‑1 (MQ=255)                                                            cAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTACTGGCTGTACTCCCgg                                                                         >  2:546084/1‑139 (MQ=255)                                                            cAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACgg                                                                         >  2:447963/1‑139 (MQ=255)                                                               cGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        >  1:92041/1‑121 (MQ=255)                                                               cGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTaa                                                                                        <  2:92041/121‑1 (MQ=255)                                                               cGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCAc                                                                      <  1:201465/139‑1 (MQ=255)                                                                   ataataAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTtg                                                                   >  2:132271/1‑138 (MQ=255)                                                                            cAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGaaatgaaatg                                                         >  1:859168/1‑139 (MQ=255)                                                                            cAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGaaatgaaatg                                                         >  1:672277/1‑139 (MQ=255)                                                                            cAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGaaatgaaatg                                                         >  1:460716/1‑139 (MQ=255)                                                                             aGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGc                                                        <  2:438325/139‑1 (MQ=255)                                                                             aGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGc                                                        <  1:106803/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  1:132271/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:992797/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:206991/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  1:705608/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:93841/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:672277/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  1:926275/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:859168/139‑1 (MQ=255)                                                                               ccACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGca                                                       >  2:318566/1‑138 (MQ=255)                                                                                             aGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACg                                                                          >  2:532431/1‑105 (MQ=255)                                                                                             aGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACg                                                                          <  1:532431/105‑1 (MQ=255)                                                                                             aGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTA‑CATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCg                                       >  2:901859/1‑139 (MQ=255)                                                                                               cgcCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGAc                                                                                                                          <  2:1094306/55‑1 (MQ=255)                                                                                               cgcCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGAc                                                                                                                          >  1:1094306/1‑55 (MQ=255)                                                                                               cgcCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAaacaa                                                                                                             >  1:708497/1‑68 (MQ=255)                                                                                               cgcCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAaacaa                                                                                                             <  2:708497/68‑1 (MQ=255)                                                                                               cgcCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGt                                      >  2:1227828/1‑139 (MQ=255)                                                                                                  cTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTA‑CATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAgg                                  <  1:901859/139‑1 (MQ=255)                                                                                                    gTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:381150/118‑1 (MQ=255)                                                                                                    gTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      >  1:381150/1‑118 (MQ=255)                                                                                                     ttCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGa                                 <  2:1102979/138‑1 (MQ=255)                                                                                                     ttCTTTAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGa                                 <  2:668259/138‑1 (MQ=255)                                                                                                         ttAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTa                            >  2:1106312/1‑139 (MQ=255)                                                                                                          tAGAATGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGCCGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGtt                             <  1:405675/137‑1 (MQ=255)                                                                                                               tGGCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGAt                       >  1:1284262/1‑138 (MQ=255)                                                                                                                 gCAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAg                    <  2:1284262/139‑1 (MQ=255)                                                                                                                  cAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGAt                       >  1:1064242/1‑135 (MQ=255)                                                                                                                  cAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGAt                       >  2:739989/1‑135 (MQ=255)                                                                                                                  cAAAACACCGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGa                   <  1:1106312/139‑1 (MQ=255)                                                                                                                          cGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      <  2:865494/96‑1 (MQ=255)                                                                                                                          cGTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGcac                                                      >  1:865494/1‑96 (MQ=255)                                                                                                                           gTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGt                                                                                          >  1:560724/1‑59 (MQ=255)                                                                                                                           gTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGt                                                                                          <  2:560724/59‑1 (MQ=255)                                                                                                                           gTCGCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAg           >  1:316831/1‑138 (MQ=255)                                                                                                                              gCCCAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAg       <  2:318687/139‑1 (MQ=255)                                                                                                                               cccAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTCCCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAgg      <  1:546084/139‑1 (MQ=255)                                                                                                                               cccAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGtt                             >  1:3304/1‑116 (MQ=255)                                                                                                                               cccAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGtt                             <  2:3304/116‑1 (MQ=255)                                                                                                                               cccAGGCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAgg      <  1:1085782/139‑1 (MQ=255)                                                                                                                                   ggCGACCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAGGCGGt  <  2:1081030/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TTCCTGTTCAGCAAGCAGACAAATTGCCCCAATTACGTCGCTTAGCCTGTAGTGTGTCGCAACGGGATAATAAAACAGCCACATTTGACTTCAGCGCCTGTTCTTTAGAATGGCAAAACACCGTCGCCCAGGCGATCAGTCAGATCGACGGCCTGAAAACAACACAGTTACCATCACCAGTAATGGCTGTACTCACGGCACTTGAAATGAAATGCACAAGATATAAAGTGCGTGAGGATGTTATGGATCAGATCGTCCAGGAAGGCGGT  >  NZ_CP009273/2190374‑2190642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑