Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,217,891	C→A	S153S (TCG→TCT)	pbpG ←	D‑alanyl‑D‑alanine endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,217,891	0	C	A	100.0%	266.7 / NA	68	S153S (TCG→TCT)	pbpG	D‑alanyl‑D‑alanine endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/36);  total (32/36)

GGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCG  >  NZ_CP009273/2217759‑2218023                                                                                                                                     |                                                                                                                                     ggTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGAtttc                                                                                                                                >  2:984552/1‑139 (MQ=255)  gtagtaCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGAtttcg                                                                                                                               >  1:181551/1‑139 (MQ=255)        cTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGAtttcgctttcg                                                                                                                         >  1:370116/1‑139 (MQ=255)            aCTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcatt                                                                                                                     >  2:592357/1‑139 (MQ=255)            aCTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcatt                                                                                                                     >  1:926394/1‑139 (MQ=255)             cTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcattc                                                                                                                    <  2:701972/139‑1 (MQ=255)             cTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcatt                                                                                                                     >  2:241712/1‑138 (MQ=255)                cccGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcattcatt                                                                                                                 >  1:345893/1‑139 (MQ=255)                cccGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGcattcatt                                                                                                                 >  2:1049713/1‑139 (MQ=255)                  cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGc                                                                                                               >  1:148056/1‑139 (MQ=255)                   gATCAACGTATAATGTTTGCTGGCAATTATCAGTTTGGTTAAGTCACGGGCGGTTGAATCGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGATCATTCCGAGAGATTTCGCTTTCGCATTCATGGcc                                                                                                              <  2:886254/139‑1 (MQ=255)                   gATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGcc                                                                                                              <  2:546234/139‑1 (MQ=255)                   gATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGcc                                                                                                              <  1:325622/139‑1 (MQ=255)                           gATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATaaa                                                                                                      <  1:513746/139‑1 (MQ=255)                            atatTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAg                                                                                                     <  1:1049713/139‑1 (MQ=255)                            atatTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAg                                                                                                     <  1:78145/139‑1 (MQ=255)                                    tGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGt                                                                                              >  2:466214/1‑138 (MQ=255)                                     gCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTa                                                                                             <  1:911646/138‑1 (MQ=255)                                      cTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAac                                                                                           >  1:1200358/1‑139 (MQ=255)                                      cTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCACTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAac                                                                                           <  1:466214/139‑1 (MQ=255)                                        ggCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAacca                                                                                         >  1:916637/1‑139 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTTATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:1170538/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:156337/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:369567/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:1200358/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:198280/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  1:804580/139‑1 (MQ=255)                                         gCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  1:241712/139‑1 (MQ=255)                                           aaTGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCg                                                                                      <  1:136818/139‑1 (MQ=255)                                                   gTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATg                                                                              <  2:370116/139‑1 (MQ=255)                                                        gTTAAGTCACAGGCAGTTGAAACGTTATGCACCGCCAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCGTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTgcg                                                                         <  1:383977/139‑1 (MQ=255)                                                         ttAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTgcg                                                                         >  1:1145518/1‑138 (MQ=255)                                                              tCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGc                                                                   >  2:1229865/1‑139 (MQ=255)                                                                  gggCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  2:394487/114‑1 (MQ=255)                                                                  gggCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        >  1:394487/1‑114 (MQ=255)                                                                  gggCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTgc                                                               >  1:670994/1‑139 (MQ=255)                                                                        ttGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCcgccgc                                                         <  2:913845/139‑1 (MQ=255)                                                                           aaaCGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCcgcgcg                                                      <  1:477877/139‑1 (MQ=255)                                                                           aaaCGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCcgcgcg                                                      <  2:926394/139‑1 (MQ=255)                                                                                ttATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGtttt                                                 >  2:1142367/1‑139 (MQ=255)                                                                                  aTGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCt                                               >  2:1209874/1‑139 (MQ=255)                                                                                  aTGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCt                                               >  2:621276/1‑139 (MQ=255)                                                                                          aCAATCCGGTAGGTTCAACAAAGTGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACAt                                       >  1:118324/1‑139 (MQ=255)                                                                                          aCAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACAt                                       >  2:1073521/1‑139 (MQ=255)                                                                                            aaTCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCa                                     <  1:1142367/139‑1 (MQ=255)                                                                                              tCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGAtttcg                                                                                                                               <  1:309366/47‑1 (MQ=255)                                                                                              tCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGAtttcg                                                                                                                               >  2:309366/1‑47 (MQ=255)                                                                                                 ggTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGcc                                <  2:345893/139‑1 (MQ=255)                                                                                                  gTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAgcgc                                 >  2:565221/1‑137 (MQ=255)                                                                                                     ggTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGc                             <  1:110904/138‑1 (MQ=255)                                                                                                     ggTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGc                             <  1:95900/138‑1 (MQ=255)                                                                                                     ggTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGc                             <  2:148056/138‑1 (MQ=255)                                                                                                      gTTCAACAAAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCaa                           <  2:670994/139‑1 (MQ=255)                                                                                                           acaaAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCa                      <  1:621276/139‑1 (MQ=255)                                                                                                           acaaAGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCa                      >  1:547508/1‑139 (MQ=255)                                                                                                               aGCGCGTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATc                  >  2:478975/1‑139 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTtaataa                                                                                                       <  2:438870/52‑1 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTtaataa                                                                                                       >  1:438870/1‑52 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        >  2:258151/1‑67 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac                                                                                        <  1:258151/67‑1 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGCTAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCtt                >  2:898440/1‑139 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCtt                >  1:843803/1‑139 (MQ=255)                                                                                                                 cgcgTGTTGTTCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCtt                >  1:538502/1‑139 (MQ=255)                                                                                                                          ttCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  1:592357/139‑1 (MQ=255)                                                                                                                          ttCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  2:916637/139‑1 (MQ=255)                                                                                                                          ttCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       <  2:1145518/139‑1 (MQ=255)                                                                                                                          ttCATTCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGa       >  2:134672/1‑139 (MQ=255)                                                                                                                               tCCGAGAGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCg  >  2:259235/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                     GGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTGAAGACATCAGCGCCAGCAACAGCATATCTTTACGGCTGATTTCG  >  NZ_CP009273/2217759‑2218023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑