Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,245,489	C→T	A104V (GCG→GTG)	yeiI →	sugar kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,245,489	0	C	T	98.3%	221.4 / ‑6.4	61	A104V (GCG→GTG)	yeiI	sugar kinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (38/21);  total (40/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACAT  >  NZ_CP009273/2245355‑2245620                                                                                                                                       |                                                                                                                                    cTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCTGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTg                                                                                                                                 >  1:372208/1‑139 (MQ=255)      tAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGTGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAgg                                                                                                                            >  2:298713/1‑139 (MQ=255)         tggtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCg                                                                                                                         >  1:860097/1‑139 (MQ=255)         tggtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCg                                                                                                                         >  1:61580/1‑139 (MQ=255)           gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGt                                                                                                                        >  2:750074/1‑138 (MQ=255)           gtggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTg                                                                                                                       <  2:635653/139‑1 (MQ=255)            tggGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGa                                                                                                                      >  1:1016373/1‑139 (MQ=255)              gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACg                                                                                                                    >  2:612098/1‑139 (MQ=255)              gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACg                                                                                                                    >  2:75441/1‑139 (MQ=255)              gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACg                                                                                                                    >  1:405241/1‑139 (MQ=255)              gggACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGAGCTGTTAGGCCGTGACg                                                                                                                    >  2:999323/1‑139 (MQ=255)                  caatcaatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCa                                                                                                                <  2:145492/139‑1 (MQ=255)                   aatcaatATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCAt                                                                                                               >  2:1031195/1‑139 (MQ=255)                               tatTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGt                                                                                                    >  1:112457/1‑138 (MQ=255)                                 ttCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGAt                                                                                                 >  1:535104/1‑139 (MQ=255)                                     cgGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGc                                                                                             >  2:586146/1‑139 (MQ=255)                                      gggATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCg                                                                                            <  2:535104/139‑1 (MQ=255)                                       ggATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGa                                                                                           >  1:612430/1‑139 (MQ=255)                                        gATGGTGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGAt                                                                                          <  1:298713/139‑1 (MQ=255)                                        gATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGAt                                                                                          <  1:949560/139‑1 (MQ=255)                                                  atCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATg                                                                                >  2:247012/1‑139 (MQ=255)                                                   tCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATg                                                                                <  1:75441/138‑1 (MQ=255)                                                       ttACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGa                                                                            >  1:283675/1‑138 (MQ=255)                                                          cccGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGTGAACTg                                                                        >  2:172912/1‑139 (MQ=255)                                                             gCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTc                                                                     >  2:287716/1‑139 (MQ=255)                                                              cAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCttt                                                                                                        >  2:1253158/1‑103 (MQ=255)                                                              cAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCttt                                                                                                        <  1:1253158/103‑1 (MQ=255)                                                               aaTCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCt                                                                   >  1:488224/1‑139 (MQ=255)                                                                        ctcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGaaa                                                           <  2:612430/138‑1 (MQ=255)                                                                         tcATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcg                                                         >  2:59167/1‑139 (MQ=255)                                                                           aTCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAAcgcg                                                       <  1:586146/139‑1 (MQ=255)                                                                                  tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                >  1:714284/1‑139 (MQ=255)                                                                                  tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                >  1:104637/1‑139 (MQ=255)                                                                                  tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                >  2:1082794/1‑139 (MQ=255)                                                                                  tACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGcc                                                >  1:554990/1‑139 (MQ=255)                                                                                       ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGt                                           >  2:1030876/1‑139 (MQ=255)                                                                                       ttCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGt                                           >  2:1197319/1‑139 (MQ=255)                                                                                            tGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGa                                         <  1:82076/136‑1 (MQ=255)                                                                                            tGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGa                                         >  2:82076/1‑136 (MQ=255)                                                                                                 aGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGTCGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGAGAATGTCTcc                                 >  1:522224/1‑139 (MQ=255)                                                                                                         gcgTGGGACGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGcgt                         <  1:287716/139‑1 (MQ=255)                                                                                                                aCGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTg                  >  1:772406/1‑139 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  2:714284/139‑1 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  1:247012/139‑1 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  2:772406/139‑1 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  1:59167/139‑1 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  2:112457/139‑1 (MQ=255)                                                                                                                 cGCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGc                 <  1:535321/139‑1 (MQ=255)                                                                                                                  gCAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCa                <  1:612098/139‑1 (MQ=255)                                                                                                                   cAACATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCAt               <  1:417666/139‑1 (MQ=255)                                                                                                                    aaCATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg              >  1:381892/1‑139 (MQ=255)                                                                                                                    aaCATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg              >  1:800857/1‑139 (MQ=255)                                                                                                                    aaCATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg              >  1:1131265/1‑139 (MQ=255)                                                                                                                    aaCATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATg              >  1:642542/1‑139 (MQ=255)                                                                                                                      cATCGCCCACAATCTGGTGCTGTTAGGCCGTGCCGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAATAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGt            >  2:351117/1‑139 (MQ=255)                                                                                                                      cATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGt            <  1:489525/139‑1 (MQ=255)                                                                                                                      cATCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGt            >  1:706692/1‑139 (MQ=255)                                                                                                                        tCGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCa          <  1:1030876/139‑1 (MQ=255)                                                                                                                         cGCCCACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCa          >  1:1048521/1‑138 (MQ=255)                                                                                                                           cccACAATCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAg       >  1:304274/1‑139 (MQ=255)                                                                                                                                aaTCTGGTGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACAt  >  1:801459/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                    CTGCGTAGTGGTGGGGACAATCAATATGGATATTCGCGGGATGGCGGATATCCGTTACCCGCAATCGGCTTCTCATCCCGGTACAATTCATTGCTCAGCGGGCGGCGTGGGACGCAACATCGCCCACAATCTGGCGCTGTTAGGCCGTGACGTCCATTTGCTTTCAGTGATTGGCGATGACTTTTATGGCGAAATGCTCCTGGAAGAAACGCGCCGTGCCGGCGTGAATGTCTCCGGCTGCGTTCGTTTGCATGGTCAAAGCACAT  >  NZ_CP009273/2245355‑2245620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑