| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,250,936 | G→A | A304V (GCC→GTC) | psuG ← | pseudouridine‑5'‑phosphate glycosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,250,936 | 0 | G | A | 94.3% | 169.0 / ‑0.4 | 53 | A304V (GCC→GTC) | psuG | pseudouridine‑5'‑phosphate glycosidase |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (25/25); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCT > NZ_CP009273/2250801‑2251067 | ctcATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTTCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACaa > 2:216086/1‑139 (MQ=255)ctcATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACaa > 2:796339/1‑139 (MQ=255) aTTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAAttt > 2:1148628/1‑139 (MQ=255) tatCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTtcgct < 2:1237706/139‑1 (MQ=255) tatCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcgct < 1:414650/139‑1 (MQ=255) atCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcgctc > 2:738157/1‑139 (MQ=255) atCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcgctc > 1:907016/1‑139 (MQ=255) tAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcgctcgc > 2:386394/1‑136 (MQ=255) aaTGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAg > 2:810770/1‑138 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgt > 1:642776/1‑139 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgt > 2:1238165/1‑139 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgt > 1:759561/1‑139 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 2:535513/138‑1 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 2:339988/138‑1 (MQ=255) ttCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 2:728050/138‑1 (MQ=255) tCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgtt > 1:276700/1‑139 (MQ=255) tCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 1:452729/137‑1 (MQ=255) tCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg > 2:452729/1‑137 (MQ=255) tCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGttgttg > 2:327484/1‑138 (MQ=255) cATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGaa > 1:722026/1‑139 (MQ=255) aTGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGaa < 2:15360/138‑1 (MQ=255) ggTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAg < 1:783039/139‑1 (MQ=255) ggTAAACCCGGCGTCAGCGCCAGGGTTTGGAATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAg > 1:890335/1‑139 (MQ=255) ccaaaaCCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAg > 1:790489/4‑139 (MQ=255) ccAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGATGAACACCAGCTGGATGTTGGATTTCAgg < 1:216086/139‑1 (MQ=255) gTTTTGTTATGCTTGATGATTACGGGCGACGGCTTTCTGCCCGTCAAAAATTACCCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAAGGGCGTTGTTGAACCCCAGCTGGATGTTGGATTTCAGGCTGt < 2:1181905/139‑1 (MQ=255) gTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGt < 1:386394/139‑1 (MQ=255) gTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGt > 2:497826/1‑139 (MQ=255) ggTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg > 2:165057/1‑97 (MQ=255) ggTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 1:165057/97‑1 (MQ=255) gTATGCTTGAGGAGTACGGGCGACGGCTTTCGGCCCGTCAGAAATTAACCCGCGAGACGCGGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCAccg < 2:322227/138‑1 (MQ=255) cTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgt < 2:775686/92‑1 (MQ=255) cTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCgt > 1:775686/1‑92 (MQ=255) cTTGATGAGTACGGGCGACGGCTTTCTCCCCGTCAGAAATTACCCCGCGAGACGCTGATATTCTTTGACATTTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGt < 1:397961/138‑1 (MQ=255) cGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAAc > 1:287358/1‑139 (MQ=255) ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcg > 2:182608/1‑57 (MQ=255) ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTtcg < 1:182608/57‑1 (MQ=255) ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATgg < 1:374982/70‑1 (MQ=255) ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATgg > 2:374982/1‑70 (MQ=255) ggCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGt > 2:752738/1‑119 (MQ=255) ggCTTTCTGCCCGTCAAAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGt < 1:752738/119‑1 (MQ=255) gCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCcag < 2:1247527/139‑1 (MQ=255) cccGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAAt < 1:497826/138‑1 (MQ=255) gTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg < 1:467798/60‑1 (MQ=255) gTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCg > 2:467798/1‑60 (MQ=255) gTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCg > 2:730237/1‑139 (MQ=255) cAGAAATTACCCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACCCCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTa < 2:1119715/139‑1 (MQ=255) gAAATTACCCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACCCCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTAct < 1:1204893/139‑1 (MQ=255) aaCCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttctt > 1:854815/1‑138 (MQ=255) aaCCCGCGAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTActttctt > 2:101107/1‑138 (MQ=255) cgAGACGCTGATATTCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAAt < 2:854815/139‑1 (MQ=255) gagcCGCTGTTATTCTTTGACATTTCCGCTCGCCAAAATGGCGTTGTTGAACACCAGCGGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATg < 2:642776/139‑1 (MQ=255) tCTTTGACAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGTGGTTGGATTCCAGGCGGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGagct < 2:424684/138‑1 (MQ=255) | CTCATTATATCCATAATGATTTTCCCTTCATGCCGGTAAACCCGGCGTCAGCGCCAGGTTTTGGTATGCTTGATGAGTACGGGCGACGGCTTTCTGCCCGTCAGAAATTAACCCGCGAGACGCTGATATTCTTTGGCAATTTCGCTCGCCAGAATGGCGTTGTTGAACACCAGCTGGATGTTGGATTTCAGGCTGTCACCGCCGGTCAGTTCAGCAACGCGCGCCAGCAGGAATGGCGTACTTTCTTTACCAATGACTCCCTGAGCT > NZ_CP009273/2250801‑2251067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |