| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,368,479 | T→C | I2V (ATC→GTC) *321W (TGA→TGG) |
menE ← menC ← |
o‑succinylbenzoate‑‑CoA ligase o‑succinylbenzoate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,368,479 | 0 | T | C | 91.9% | 116.1 / 1.9 | 37 | I2V (ATC→GTC) *321W (TGA→TGG) | menE menC | o‑succinylbenzoate‑‑CoA ligase o‑succinylbenzoate synthase |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (16/18); total (17/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
CACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTT > NZ_CP009273/2368345‑2368599 | cACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCAt > 2:875651/1‑138 (MQ=255) ccGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATaac < 1:778562/139‑1 (MQ=255) gCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATaacaa > 2:973450/1‑139 (MQ=255) aTCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGc > 1:94952/1‑139 (MQ=255) cTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCa < 1:637467/139‑1 (MQ=255) aTCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACtt < 1:559993/139‑1 (MQ=255) cacaAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAgg > 2:1216969/1‑138 (MQ=255) acaAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCa < 1:717849/139‑1 (MQ=255) aGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGt < 1:340590/139‑1 (MQ=255) cTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGc < 1:1163051/139‑1 (MQ=255) cgcCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTAcc > 2:53815/1‑139 (MQ=255) agttgagttgCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGc < 1:1006119/139‑1 (MQ=255) tAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCtgctgc > 1:902823/1‑139 (MQ=255) aGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTgcgc < 2:94952/139‑1 (MQ=255) tAAGGCTATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCATCGACGTACCTGCTGCGCCTGCa > 2:1052966/1‑139 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGt > 2:553937/1‑104 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGt < 1:553937/104‑1 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCa > 1:1047161/1‑139 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCa > 2:25491/1‑139 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCa > 2:229757/1‑139 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCa > 2:1221450/1‑139 (MQ=255) tAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCa < 1:25491/139‑1 (MQ=255) aGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCAt < 2:1047161/138‑1 (MQ=255) gATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGAt < 1:268576/139‑1 (MQ=255) aTGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCAt < 2:174929/133‑1 (MQ=255) aTGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCAt > 1:174929/1‑133 (MQ=255) tcCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCgtgt > 1:876126/1‑139 (MQ=255) cccGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTc < 1:1216969/138‑1 (MQ=255) cccGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGATCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTc < 1:875651/138‑1 (MQ=255) ccGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCa < 2:876126/139‑1 (MQ=255) tGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTg > 1:703053/1‑138 (MQ=255) gccgccAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTgg > 2:94183/1‑138 (MQ=255) ccgccAGTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGaa < 2:1260199/139‑1 (MQ=255) gTGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGgtgt < 1:667643/139‑1 (MQ=255) tGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTc > 1:689761/1‑139 (MQ=255) tGACGCCACGGCCAGTCAGAGAAGACCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTc < 1:438721/139‑1 (MQ=255) aCGGCCAGTCAGAGAAGACCATAACACCCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGAGCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGtt < 2:91520/139‑1 (MQ=255) | CACCGCAAATCCGGAGGCTAATTCATCGACGCGAGCACAAAGCTCGCGCCAGTTGAGTTGCTCGTCATTAAGACGTAAGGCGATGGTTTCTCCCCGCACTTGCCGCCAGTGACGCCACGGCCAGTCAGAGAAGATCATAACAACCGCTCCAGTGCATCAACTTCCACGACAGGCAGCGTGCTACCCGGCCAGCGACGTACCTGCTGCGCCTGCATCAGATCCAGCGTGTCCAGCCCTGGAATGGTGTCCGGCGTT > NZ_CP009273/2368345‑2368599 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |