| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,370,899 | A→T | V58D (GTC→GAC) | menH ← | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,370,899 | 0 | A | T | 91.1% | 154.8 / 6.3 | 45 | V58D (GTC→GAC) | menH | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base T (27/14); total (29/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAC > NZ_CP009273/2370779‑2371034 | tGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAac > 2:512831/1‑139 (MQ=255) gccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGt > 2:407026/1‑139 (MQ=255) ccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCACGGATGTTGGAACTAACCAAGGCTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGt > 1:460109/1‑138 (MQ=255) ccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCg > 2:549840/1‑137 (MQ=255) ccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCg < 1:549840/137‑1 (MQ=255) ccgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGt > 2:476666/1‑138 (MQ=255) cgccATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGa > 2:882698/1‑139 (MQ=255) ccATCAGCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGAcc < 2:68375/139‑1 (MQ=255) catcatCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCt < 1:407026/139‑1 (MQ=255) cGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg > 2:1140942/1‑135 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 1:882698/139‑1 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 1:1140942/139‑1 (MQ=255) gCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 1:711296/139‑1 (MQ=255) cACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATc > 2:577524/1‑138 (MQ=255) ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGaa > 2:695353/1‑115 (MQ=255) ccGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGaa < 1:695353/115‑1 (MQ=255) gTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa > 1:661417/1‑139 (MQ=255) tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa < 2:1026215/138‑1 (MQ=255) tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa > 1:1026215/1‑138 (MQ=255) tCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATa > 1:703369/1‑138 (MQ=255) caccaGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGc > 2:864548/1‑138 (MQ=255) ccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGcc < 1:955156/139‑1 (MQ=255) aaGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCAct > 1:704683/1‑139 (MQ=255) tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCa > 1:49363/1‑139 (MQ=255) taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt > 1:1112007/1‑88 (MQ=255) taactaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGAt < 2:1112007/88‑1 (MQ=255) actaacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCg > 1:73267/1‑139 (MQ=255) taacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATcgc < 1:1198382/58‑1 (MQ=255) taacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATcgc > 2:1198382/1‑58 (MQ=255) aacCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTgg > 1:1193514/1‑139 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa < 2:954696/81‑1 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa < 1:1064987/81‑1 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa > 2:1064987/1‑81 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACa > 1:954696/1‑81 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg < 2:420436/67‑1 (MQ=255) tACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTgg > 1:420436/1‑67 (MQ=255) tAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg < 2:881604/60‑1 (MQ=255) tAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg > 1:881604/1‑60 (MQ=255) tAAGTCGGTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCg > 2:478672/1‑139 (MQ=255) gTGACATCATCAAATCCATCGTCGCTAATCGCGGCCGAACCACCGTGACCTgg < 1:232521/53‑1 (MQ=255) gTGACATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTgg > 2:232521/1‑53 (MQ=255) aCATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAaca > 1:606315/1‑139 (MQ=255) aCATCATCAAATCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAaca > 2:730421/1‑139 (MQ=255) tCCATCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAggg > 1:191573/1‑138 (MQ=255) aTCGTCGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTaaa > 2:769136/1‑139 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTgg > 1:300547/1‑35 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTgg < 2:300547/35‑1 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGcc < 1:890553/77‑1 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGcc > 2:890553/1‑77 (MQ=255) tcgtcgCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAc > 2:149545/1‑139 (MQ=255) | TGGCAAGCCGCCATCATCGCCACCCGTCCACCAAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCTCGCCCACTTCTTGCCATTCGTGGCAATCGCCGGAAAAACCGTGCAAAAACACCAGCCAGGGTAAAC > NZ_CP009273/2370779‑2371034 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |