| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,400,959 | A→C | intergenic (‑839/‑81) | lrhA ← / → alaA | transcriptional regulator LrhA/alanine transaminase AlaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,400,959 | 0 | A | C | 97.1% | 123.0 / ‑3.2 | 35 | intergenic (‑839/‑81) | lrhA/alaA | transcriptional regulator LrhA/alanine transaminase AlaA |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (23/11); total (24/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
GAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTAT > NZ_CP009273/2400829‑2401084 | gAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTcc < 2:831949/139‑1 (MQ=255) cATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCt > 2:1122162/1‑139 (MQ=255) tGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGAc > 1:1225127/1‑139 (MQ=255) tGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGAc > 1:890244/1‑139 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTgggg < 2:63708/115‑1 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTgggg > 1:63708/1‑115 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACa < 2:32949/122‑1 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACa > 1:32949/1‑122 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACtt > 2:1048061/1‑139 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACtt > 1:176665/1‑139 (MQ=255) cATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCACTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACtt > 2:1213634/1‑139 (MQ=255) atatACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAgg > 2:1278573/1‑139 (MQ=255) tCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCa < 2:331020/128‑1 (MQ=255) tCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCa > 1:331020/1‑128 (MQ=255) tGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAcca > 2:357801/1‑76 (MQ=255) tGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAcca < 1:357801/76‑1 (MQ=255) tGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCa > 2:823415/1‑138 (MQ=255) tGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCa > 2:405429/1‑138 (MQ=255) ttaAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGt < 2:492567/138‑1 (MQ=255) gTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTATTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGa < 1:444675/139‑1 (MQ=255) aGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCaaaaa < 2:1247333/121‑1 (MQ=255) aGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCaaaaa > 1:1247333/1‑121 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTc < 1:574030/89‑1 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTc > 2:574030/1‑89 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTcccc > 1:730341/1‑139 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTcccc > 1:560522/1‑139 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTcccc > 2:577643/1‑139 (MQ=255) cacaATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTAATGTccac > 2:598872/1‑137 (MQ=255) tCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCAtt > 1:1172601/1‑138 (MQ=255) ccTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAcca > 1:1143602/1‑43 (MQ=255) ccTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAcca < 2:1143602/43‑1 (MQ=255) tCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATc > 1:336776/1‑138 (MQ=255) ctGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa < 2:180770/72‑1 (MQ=255) ctGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCa > 1:180770/1‑72 (MQ=255) tGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACa < 2:20532/51‑1 (MQ=255) tGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACa > 1:20532/1‑51 (MQ=255) tGGCAAAATCTTATTCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAAt > 1:1042664/1‑139 (MQ=255) ttCTGCAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCt < 2:560522/138‑1 (MQ=255) cAGACCTTCAAACCACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTAt > 2:1166237/1‑138 (MQ=255) | GAATCAGAACAATTCACTATATAACATTGCATGTAAAGCATATACACCTCATTATTTTGTCATTATTAAGTTATTAACAGCACAATCGAGCCTTCCCCTCTGGCAAAATCTTATTCTGCAGACCTTCAAAACACCGTCCTGGGGGAGTACATTGTTCTAAGCTGACTTCCACGGCAGGGAGTGGCGATAACAGCAAAAAAGGTCAAGATTCATGTCCCCCATTGAAAAATCCAGCAAATTAGAGAATGTCTGTTAT > NZ_CP009273/2400829‑2401084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |