| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,441,796 | A→C | W42G (TGG→GGG) | prmB ← | 50S ribosomal protein L3 N(5)‑glutamine methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,441,796 | 0 | A | C | 96.9% | 103.7 / ‑4.2 | 32 | W42G (TGG→GGG) | prmB | 50S ribosomal protein L3 N(5)‑glutamine methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (15/16); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
CGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCC > NZ_CP009273/2441672‑2441929 | cggcggGTCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGCAGGCCACCCCAGCTCTACGGCTTCATCCCCCCGGCTATCGGTAc > 2:1107609/1‑139 (MQ=255) ggcggATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTAcc < 2:1166680/139‑1 (MQ=255) gATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGa < 2:945790/139‑1 (MQ=255) gcgTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTCCATCCCCCGGGTTATCGGTACCGTGACCGTAc < 1:1107609/139‑1 (MQ=255) caacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCgg < 2:479413/112‑1 (MQ=255) caacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCgg > 1:479413/1‑112 (MQ=255) caacaaTACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGa > 2:459811/1‑138 (MQ=255) ggTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCcgc < 1:93996/139‑1 (MQ=255) tttttCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCt > 1:851003/1‑138 (MQ=255) cGCTGGAGGTCAGACGCGCGGTGCGCATATCTGCCGGAATATCCAGCGGCAGGTAGAGCG‑AGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCg < 1:939799/138‑1 (MQ=255) tGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCt > 1:244196/1‑139 (MQ=255) aGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc < 1:363080/138‑1 (MQ=255) aGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc < 2:435611/138‑1 (MQ=255) aGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTGATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGAc < 2:1059161/138‑1 (MQ=255) ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg > 1:714329/1‑139 (MQ=255) ttCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATg > 2:386840/1‑139 (MQ=255) ccGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGt > 2:503525/1‑139 (MQ=255) aaTATCCAGCGGCAGGTACAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATAGTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCCGAATGGTTTGc < 2:966795/139‑1 (MQ=255) tatCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAg < 2:564769/139‑1 (MQ=255) ggCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTa > 1:192625/1‑139 (MQ=255) gCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTAc < 2:851003/139‑1 (MQ=255) aGGCAACACCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAAc > 1:463319/1‑138 (MQ=255) acacCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAAt > 1:838485/1‑139 (MQ=255) cacCAGCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAAtt > 1:461867/1‑139 (MQ=255) gCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATc > 1:898629/1‑139 (MQ=255) gCTGTACGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATc < 2:463319/139‑1 (MQ=255) tACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCa < 2:635236/137‑1 (MQ=255) aCGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTa < 1:626308/132‑1 (MQ=255) aCGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTa > 2:626308/1‑132 (MQ=255) cGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTa > 2:112377/1‑139 (MQ=255) cGGCTTCATCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTa < 2:192625/139‑1 (MQ=255) aTCCCCCGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATtcctcc < 1:459811/139‑1 (MQ=255) | CGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGGAGGTCAGACGCGCGGTGCGCATATCTTCCGGAATATCCAGCGGCAGGTAGAGCGAAGGCAACACCAGCTGTACGGCTTCATCCCACGGGTTATCGGTACCGTGACCGTACCAGATATTTGCCGCGCTGAAGCGGCTCACCGACCAGCGCAACATGTCCTGAATGGTTTGCAGCTCATTTACTGCTTCATCAACGAAAATTTTATCCACGTATTCCTCC > NZ_CP009273/2441672‑2441929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |