| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 183,409 | A→G | F577L (TTT→CTT) | glnD ← | bifunctional uridylyltransferase/uridylyl‑removing protein GlnD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 183,409 | 0 | A | G | 97.5% | 137.0 / ‑4.0 | 40 | F577L (TTT→CTT) | glnD | bifunctional uridylyltransferase/uridylyl‑removing protein GlnD |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (19/20); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
CAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCA > NZ_CP009273/183280‑183540 | cAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTtgat < 2:990745/139‑1 (MQ=255) gagCTCACGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTcc > 1:349761/1‑139 (MQ=255) cTCACGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCggg > 2:393355/1‑139 (MQ=255) aCGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTcc > 1:660724/1‑139 (MQ=255) aCGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTc < 1:638921/138‑1 (MQ=255) aCGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTc < 2:129558/138‑1 (MQ=255) gCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTcc > 1:527612/1‑131 (MQ=255) gCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTcc < 2:527612/131‑1 (MQ=255) gCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCt > 2:107788/1‑138 (MQ=255) aCTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATcg < 1:166529/139‑1 (MQ=255) aCTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATcg < 2:530939/139‑1 (MQ=255) gCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCgcggcg > 2:140852/1‑139 (MQ=255) ccAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCCTGATGACTTCCGGGTCCTGAATATCGCGGCGTTg < 1:449650/138‑1 (MQ=255) tttCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCaa < 2:172154/139‑1 (MQ=255) ggCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAAc < 2:519582/130‑1 (MQ=255) ggCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAAc > 1:519582/1‑130 (MQ=255) gcgcAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGc > 2:429195/1‑139 (MQ=255) gCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAgg > 2:1071498/1‑139 (MQ=255) caGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGAc < 1:670297/139‑1 (MQ=255) gTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGTTGCTGGCGAACCAGCCAGGCGAc > 1:707661/1‑137 (MQ=255) aGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGc < 2:457688/138‑1 (MQ=255) aCCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGAc > 2:721695/1‑128 (MQ=255) aCCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGAc < 1:721695/128‑1 (MQ=255) ccAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCg > 2:476762/1‑93 (MQ=255) ccAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCg < 1:476762/93‑1 (MQ=255) gATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTcgcg < 1:955054/139‑1 (MQ=255) aTAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTcc > 1:749297/1‑55 (MQ=255) aTAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTcc < 2:749297/55‑1 (MQ=255) tAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGt > 1:1275337/1‑138 (MQ=255) aGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTcgcg > 2:1222777/1‑136 (MQ=255) aGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTcgcg < 1:1222777/136‑1 (MQ=255) aGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGt > 2:306064/1‑137 (MQ=255) cgcAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGt < 1:1137744/139‑1 (MQ=255) cgcAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGt > 1:711539/1‑139 (MQ=255) cgcAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGt < 2:9459/139‑1 (MQ=255) cAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGtt > 2:771387/1‑138 (MQ=255) cAGACGATTTTCCGTTTGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTc < 2:1003992/139‑1 (MQ=255) tGCACTTCTTCGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCgg > 2:318676/1‑139 (MQ=255) cGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCa < 2:385393/138‑1 (MQ=255) cGGCAAGCTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCa < 2:711539/138‑1 (MQ=255) | CAAAGTAGAGCTCACGCAACAGACTTTGCTTCCAGCTATTCCACAGCGTTTCGTTGGTGGCGCAAATGTCAGCCACAGTCAGGCATACCAGATAGCGCAGACGATTTTCCGTTTGCACTTCTTCGGCAAACTGCTTGATGACTTCCGGGTCCTGAATATCGCGGCGTTGGGCGGTCACCGACATCAACAGGTGCTGGCGAACCAGCCAGGCGACCAGCTGTGTTTCGCGTGAGTTCAGCCCGTGGAGTTCGGCAAAATGCA > NZ_CP009273/183280‑183540 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |