| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,451,709 | G→T | S310R (AGC→AGA) | fadJ ← | fatty acid oxidation complex subunit alpha FadJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,451,709 | 0 | G | T | 95.2% | 148.0 / ‑0.2 | 42 | S310R (AGC→AGA) | fadJ | fatty acid oxidation complex subunit alpha FadJ |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (24/16); total (25/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
TACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAA > NZ_CP009273/2451587‑2451844 | tACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAg > 2:943755/1‑139 (MQ=255) cTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAggc > 1:400609/1‑139 (MQ=255) cTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAgg < 2:400609/138‑1 (MQ=255) cAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAggcgg < 1:1064073/138‑1 (MQ=255) aGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGgcggcg < 1:822620/139‑1 (MQ=255) cgcATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCa > 1:1164668/1‑139 (MQ=255) cgcATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCa > 1:1246521/1‑139 (MQ=255) cgcATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCa > 2:479516/1‑139 (MQ=255) cgcATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCa > 1:92889/1‑139 (MQ=255) cTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtct > 2:99516/1‑139 (MQ=255) cTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtct < 2:1164668/139‑1 (MQ=255) cTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtct > 2:100223/1‑139 (MQ=255) tGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctt > 1:1091855/1‑139 (MQ=255) ggTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctttctt < 2:981084/139‑1 (MQ=255) ggTTGATATCTTTAATTAGGAACGGAATCCCCGCTTTACAAGCAGTCACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctttctt < 2:553334/139‑1 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaa > 2:237672/1‑138 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaa > 1:122012/1‑138 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaa < 1:195002/137‑1 (MQ=255) aCCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCaaaaa < 1:152836/139‑1 (MQ=255) ccGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTCCTGGCAAAAAAGATACTACg > 2:1286118/1‑139 (MQ=255) ccGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACg < 1:943755/139‑1 (MQ=255) gCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACg > 2:360451/1‑137 (MQ=255) cAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCt < 1:340246/139‑1 (MQ=255) gCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGc > 2:986508/1‑138 (MQ=255) gTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGAtt < 2:340143/139‑1 (MQ=255) aaTACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCa > 1:32446/1‑116 (MQ=255) aaTACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCa < 2:32446/116‑1 (MQ=255) tACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGc > 1:903602/1‑139 (MQ=255) tACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGc > 2:794646/1‑139 (MQ=255) tACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGc > 1:638684/1‑139 (MQ=255) tACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGc > 2:1069010/1‑139 (MQ=255) tACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGc > 1:240873/1‑139 (MQ=255) ccgccgCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCa > 1:1149665/1‑112 (MQ=255) ccgccgCCCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCa < 2:1149665/112‑1 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGTCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGtt > 1:274269/1‑139 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGc < 1:365935/64‑1 (MQ=255) ccgccCATCAAGCCACCACCTAAAATCCCCACTATGTTTAATGGCGCAGGCGGCGCATCACTGc > 2:365935/1‑64 (MQ=255) ccaccTAAAATCCCCACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAg < 1:971440/139‑1 (MQ=255) cccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctttctt > 2:372356/1‑50 (MQ=255) cccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGAtctttctt < 1:372356/50‑1 (MQ=255) cccACTCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCAt < 1:360451/139‑1 (MQ=255) cACTCTGTTTAATGGCGCAGACGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAAATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCGTCGGCGTCATaa > 1:92666/1‑139 (MQ=255) | TACTTCAGCGCATGATTTATGCCCTGCGGGTTGATATCTTTAATTCTGACCGGAATCCCCGCTTTACAAGCAGTGACATAAGCAATACCGCCGCCCATCAAGCCACCACCTAAAATCCCCACGCTGTTTAATGGCGCAGGCGGCGCATCACTGCCGGGATCTTTCTTCACGTCCGTACTGGCAAAAAAGATACTACGCAGCGCCTGCGATTGTGGCGTCATCGCCAGTTCGCCAAACGCCCGAGCTTCGGCGTCATAA > NZ_CP009273/2451587‑2451844 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |