Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,453,267 T→C T228A (ACT→GCT)  fadI ← acetyl‑CoA C‑acyltransferase FadI

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,453,2670TC100.0% 138.4 / NA 37T228A (ACT→GCT) fadIacetyl‑CoA C‑acyltransferase FadI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/19);  total (18/19)

GTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCG  >  NZ_CP009273/2453136‑2453402
                                                                                                                                   |                                                                                                                                       
gTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCAcc                                                                                                                                  <  2:439045/139‑1 (MQ=255)
 tttGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACct                                                                                                                                 <  1:1159128/139‑1 (MQ=255)
 tttGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACct                                                                                                                                 >  1:623029/1‑139 (MQ=255)
              ccGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGtt                                                                                                                     <  1:1151588/138‑1 (MQ=255)
                    cGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGt                                                                                                               <  1:632125/138‑1 (MQ=255)
                     gtgtTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCt                                                                                                             >  2:492306/1‑139 (MQ=255)
                        tttGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGAc                                                                                                          <  2:623029/139‑1 (MQ=255)
                        tttGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGAc                                                                                                          <  2:572426/139‑1 (MQ=255)
                            cGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATg                                                                                                      >  1:973669/1‑139 (MQ=255)
                             gATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGc                                                                                                     <  2:295600/139‑1 (MQ=255)
                                         ggCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACg                                                                                         <  1:1146291/139‑1 (MQ=255)
                                                    gCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAAc                                                                              >  1:1282298/1‑139 (MQ=255)
                                                    gCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAAc                                                                              >  2:57446/1‑139 (MQ=255)
                                                     cGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACg                                                                             <  2:888740/139‑1 (MQ=255)
                                                      gTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACgg                                                                            <  2:1109354/139‑1 (MQ=255)
                                                          tCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGt                                                                                                               <  1:1151001/100‑1 (MQ=255)
                                                          tCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGt                                                                                                               >  2:1151001/1‑100 (MQ=255)
                                                          tCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTgcg                                                                        >  2:859371/1‑139 (MQ=255)
                                                          tCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTgcg                                                                        >  1:299577/1‑139 (MQ=255)
                                                               aaGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAAt                                                                   >  1:1264365/1‑139 (MQ=255)
                                                                    aGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATc                                                              >  2:1131819/1‑139 (MQ=255)
                                                                      gAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACgg                                                                            <  2:1188105/123‑1 (MQ=255)
                                                                      gAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACgg                                                                            >  1:1188105/1‑123 (MQ=255)
                                                                      gAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACg                                                                             >  1:76628/1‑122 (MQ=255)
                                                                      gAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACg                                                                             <  2:76628/122‑1 (MQ=255)
                                                                                     ttgttgTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTg                                              <  1:1271726/138‑1 (MQ=255)
                                                                                     ttgttgTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGa                                             <  2:1219586/139‑1 (MQ=255)
                                                                                           tCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCg                                        <  1:57446/138‑1 (MQ=255)
                                                                                                     gCGGTTGTTTATAAGGAGGGATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGc                             >  2:794952/1‑139 (MQ=255)
                                                                                                                      gggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTg                                                                          >  1:57468/1‑77 (MQ=255)
                                                                                                                      gggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTg                                                                          <  2:57468/77‑1 (MQ=255)
                                                                                                                      gggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTa                                                                     >  2:1227113/1‑82 (MQ=255)
                                                                                                                      gggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTa                                                                     <  1:1227113/82‑1 (MQ=255)
                                                                                                                      gggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGgtgt            >  2:520767/1‑139 (MQ=255)
                                                                                                                       ggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTc           >  2:350400/1‑139 (MQ=255)
                                                                                                                       ggATAAAGGCAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTc           >  1:99667/1‑139 (MQ=255)
                                                                                                                                cAGCCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCg  <  2:299577/139‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                       
GTTTGCCGCCGTTACCGTTCCGTGTTTGCGATCAAACGCCGGGCGCAGCTTTGCGTAATCGGCAAGCGAGGAATTACCGCGAATATTGTTGTCTTCGACAAGCGGTTGTTTATAAGGAGGGATAAAGGCAGTCATCACCTCTTCTTTGAGTTTTCCGTCTGACCATGCCTGAGCGGCACGCTGATGCGAACGGTGCGCTAATGCATCTTGCTGTTCTCGGGTGATGCCGTAGGTTTTCGCCATTTGCTCTGCGGTGTCGCCCATCCG  >  NZ_CP009273/2453136‑2453402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑