Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,484,113 A→G S439S (TCT→TCC)  oxc ← oxalyl‑CoA decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,484,1130AG94.8% 254.0 / 4.4 77S439S (TCT→TCC) oxcoxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base G (26/47);  total (27/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCA  >  NZ_CP009273/2483979‑2484227
                                                                                                                                      |                                                                                                                  
tCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAAc                                                                                                                <  1:1153193/139‑1 (MQ=255)
  aaCACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAAc                                                                                                                >  1:46438/1‑137 (MQ=255)
    cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAg                                                                                                             >  1:250311/1‑138 (MQ=255)
    cacCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAg                                                                                                             >  1:636276/1‑138 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  2:46438/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  2:219151/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:559568/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:16050/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:142149/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:1266049/139‑1 (MQ=255)
       cGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  2:935650/139‑1 (MQ=255)
         tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCa                                                                                                       >  1:113451/1‑139 (MQ=255)
         tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCa                                                                                                       <  1:313768/139‑1 (MQ=255)
         tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGt                                                                                                         <  1:1273046/137‑1 (MQ=255)
         tctcCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGt                                                                                                         >  2:1273046/1‑137 (MQ=255)
                    gATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                            <  1:791131/139‑1 (MQ=255)
                       gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:1046373/123‑1 (MQ=255)
                       gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         >  2:1046373/1‑123 (MQ=255)
                       gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                            <  2:623447/136‑1 (MQ=255)
                       gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                            >  1:623447/1‑136 (MQ=255)
                       gccgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATg                                                                                         >  1:288362/1‑139 (MQ=255)
                         cgccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                       <  2:88796/139‑1 (MQ=255)
                          gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                            >  1:1060436/1‑133 (MQ=255)
                          gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCg                                                                                            <  2:1060436/133‑1 (MQ=255)
                          gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      <  1:223531/139‑1 (MQ=255)
                          gccATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      <  2:260254/139‑1 (MQ=255)
                              ttattaAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGc                                                                                  <  2:250311/139‑1 (MQ=255)
                                      aaTAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                          <  2:1229116/139‑1 (MQ=255)
                                        tAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGAt                                                                        >  2:227113/1‑139 (MQ=255)
                                         aaCGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCa                                                                               >  2:868896/1‑131 (MQ=255)
                                         aaCGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCa                                                                               <  1:868896/131‑1 (MQ=255)
                                           cGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGcc                                                                     <  2:842350/139‑1 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCAc                                                                                              <  2:3843/109‑1 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCAc                                                                                              >  1:3843/1‑109 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                <  2:356469/139‑1 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                >  1:356469/1‑139 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                <  2:872361/139‑1 (MQ=255)
                                                gTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                <  1:1247147/139‑1 (MQ=255)
                                                 tCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGac                                                               <  2:636276/139‑1 (MQ=255)
                                                 tCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGac                                                               <  1:416536/139‑1 (MQ=255)
                                                      ggCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcccc                                                          <  1:605282/139‑1 (MQ=255)
                                                        cAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      <  1:658791/109‑1 (MQ=255)
                                                        cAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      >  2:658791/1‑109 (MQ=255)
                                                         aGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAgg                                                       <  1:138975/139‑1 (MQ=255)
                                                           gTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                <  1:949249/128‑1 (MQ=255)
                                                           gTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGa                                                                >  2:949249/1‑128 (MQ=255)
                                                                 tCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACcaca                                                <  2:1095251/138‑1 (MQ=255)
                                                                     cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            >  1:919654/1‑122 (MQ=255)
                                                                     cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  2:919654/122‑1 (MQ=255)
                                                                     cAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATc                                             >  1:749934/1‑137 (MQ=255)
                                                                         tCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgac                                        >  2:108396/1‑138 (MQ=255)
                                                                               cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:545/138‑1 (MQ=255)
                                                                               cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:242868/138‑1 (MQ=255)
                                                                               cAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  2:113451/138‑1 (MQ=255)
                                                                                aaTTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGacgacgacg                                 <  1:880788/138‑1 (MQ=255)
                                                                                           cACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACat                     <  1:108396/139‑1 (MQ=255)
                                                                                            aCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         >  2:639972/1‑54 (MQ=255)
                                                                                            aCTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGt                                                                                                         <  1:639972/54‑1 (MQ=255)
                                                                                              tGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACca                                                  <  1:733343/107‑1 (MQ=255)
                                                                                              tGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACca                                                  >  2:733343/1‑107 (MQ=255)
                                                                                                            cTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  1:1245897/109‑1 (MQ=255)
                                                                                                            cTATCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  >  2:1245897/1‑109 (MQ=255)
                                                                                                               tCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCa  <  1:744116/138‑1 (MQ=255)
                                                                                                               tCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCa  <  2:212019/138‑1 (MQ=255)
                                                                                                               tCACCTTCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCa  <  2:82745/138‑1 (MQ=255)
                                                                                                                    ttCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      >  2:533371/1‑49 (MQ=255)
                                                                                                                    ttCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      <  1:533371/49‑1 (MQ=255)
                                                                                                                     tCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      <  1:215857/48‑1 (MQ=255)
                                                                                                                     tCAATGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCa                                                                                      >  2:215857/1‑48 (MQ=255)
                                                                                                                       aaTGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  <  2:603382/98‑1 (MQ=255)
                                                                                                                       aaTGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAgacgacgac                                  >  1:603382/1‑98 (MQ=255)
                                                                                                                         tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            >  2:254652/1‑70 (MQ=255)
                                                                                                                         tGGCGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACAcc                                                            <  1:254652/70‑1 (MQ=255)
                                                                                                                            cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                       <  1:603211/40‑1 (MQ=255)
                                                                                                                            cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGc                                                                                       >  2:603211/1‑40 (MQ=255)
                                                                                                                            cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                          <  2:178380/53‑1 (MQ=255)
                                                                                                                            cGACAACCGGGGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCg                                                                          >  1:178380/1‑53 (MQ=255)
                                                                                                                                      |                                                                                                                  
TCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCACTATCACCTTCAATGGCGACAACCGGAGAACCAGAAGTCACGCTAGCACCGATGGCATAGCCCATACCGATGCCCATGACACCCCAGGTACCACAATCCAGACGACGACGTGGTTTATACATATCAATAATATTTCGTGCA  >  NZ_CP009273/2483979‑2484227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑