| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,519,318 | G→C | R300G (CGG→GGG) | yfeR ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,519,318 | 0 | G | C | 86.8% | 160.0 / 19.6 | 53 | R300G (CGG→GGG) | yfeR | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (3/4); new base C (23/23); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01 | |||||||||||
CGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATGCGCTTTACCACCAG > NZ_CP009273/2519183‑2519453 | cGGATGACAAACTGGGCGAAGGTTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACATCGTATCCCCTTcccccc > 2:635532/1‑138 (MQ=255)cGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcccccc > 2:763277/1‑138 (MQ=255) ggATTACAAACTGGGCTAAGATTTTTCCCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCa < 2:864711/139‑1 (MQ=255) gATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAg > 1:974766/1‑139 (MQ=255) gACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCa > 1:588728/1‑139 (MQ=255) aCAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCa < 1:48384/138‑1 (MQ=255) cAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCAtt < 1:98681/139‑1 (MQ=255) gggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCa > 1:1003200/1‑139 (MQ=255) ggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 2:703743/138‑1 (MQ=255) ggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCa < 2:588728/138‑1 (MQ=255) ggCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCa < 1:895704/138‑1 (MQ=255) gCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCAtt > 2:570114/1‑139 (MQ=255) aGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGccc < 1:734950/139‑1 (MQ=255) aGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGccc < 1:73940/139‑1 (MQ=255) aGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGccc < 1:1257177/139‑1 (MQ=255) tttACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGt < 2:884215/138‑1 (MQ=255) nncnAGGNTGCGATAATCTTCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTg < 2:412162/132‑1 (MQ=255) tGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 2:992879/139‑1 (MQ=255) cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCa < 1:61277/106‑1 (MQ=255) cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCa > 2:61277/1‑106 (MQ=255) cTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCt > 2:168238/1‑139 (MQ=255) tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt > 1:911291/1‑139 (MQ=255) tCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt > 2:646667/1‑139 (MQ=255) cATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCtt > 2:955312/1‑137 (MQ=255) tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgc > 1:883042/1‑139 (MQ=255) aaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc < 1:168238/139‑1 (MQ=255) aaCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTg < 1:1189317/101‑1 (MQ=255) aaCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTg > 2:1189317/1‑101 (MQ=255) attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa > 2:355593/1‑139 (MQ=255) attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa < 1:763277/138‑1 (MQ=255) attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGa < 1:955312/138‑1 (MQ=255) ttgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc < 1:1048927/139‑1 (MQ=255) ttgattACTACCGGGGTTCGCGGCAGTATGAATTCACTCTATATCTGATACAACGGCTCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCTTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAAc < 2:930744/139‑1 (MQ=255) gggCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg > 1:933374/1‑139 (MQ=255) gggCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg > 2:1032452/1‑139 (MQ=255) cTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAc < 2:314625/139‑1 (MQ=255) ctctATATCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTca > 2:1209641/1‑138 (MQ=255) tCTGATACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCg < 2:858467/138‑1 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt > 1:607715/1‑95 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGt < 2:607715/95‑1 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGt > 1:1214939/1‑138 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGt > 2:820840/1‑138 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGt > 1:540338/1‑138 (MQ=255) aTACAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACCGGCGTTCAACAACCGGCGt > 2:1070741/1‑138 (MQ=255) aCAACGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAAt > 2:204914/1‑139 (MQ=255) aaCGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATgc < 1:1242137/139‑1 (MQ=255) aaCGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATgc > 2:330454/1‑139 (MQ=255) cGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCCGCATCAACTGGCGTTCAACAACCGGCGTAATgcgc > 2:762572/1‑139 (MQ=255) cGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg < 1:302781/71‑1 (MQ=255) cGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcgg > 2:302781/1‑71 (MQ=255) cGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa < 1:219601/101‑1 (MQ=255) cGGATCCCCTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa > 2:219601/1‑101 (MQ=255) cccTTCCCCCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATGCGCTTTac < 1:330454/138‑1 (MQ=255) ccccccCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATGCGCTTTACCACCAg > 1:946780/1‑139 (MQ=255) cccGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa < 2:263404/90‑1 (MQ=255) cccGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCa > 1:263404/1‑90 (MQ=255) | CGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGGCGTTCAACAACCGGCGTAATGCGCTTTACCACCAG > NZ_CP009273/2519183‑2519453 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |