| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,521,249 | A→C | K315N (AAA→AAC) | yfeH → | bile acid:sodium symporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,521,249 | 0 | A | C | 97.7% | 156.6 / ‑3.3 | 44 | K315N (AAA→AAC) | yfeH | bile acid:sodium symporter |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (27/16); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.17e-01 | |||||||||||
GGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCA > NZ_CP009273/2521123‑2521378 | ggCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGaa < 2:837096/139‑1 (MQ=255) gCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGaa < 1:923030/138‑1 (MQ=255) gCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGa < 1:518654/137‑1 (MQ=255) ccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGgcg < 2:1050987/138‑1 (MQ=255) ttCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGcc > 1:1032052/1‑138 (MQ=255) cacaTCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATa > 1:636575/1‑137 (MQ=255) cGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCtt > 2:1195253/1‑138 (MQ=255) gTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGaa < 2:1122478/96‑1 (MQ=255) gTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGaa > 1:1122478/1‑96 (MQ=255) ggTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa < 1:985134/138‑1 (MQ=255) ggTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa < 2:619520/138‑1 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAgg > 2:700159/1‑88 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAgg > 2:948864/1‑88 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAgg < 1:948864/88‑1 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAgg < 1:700159/88‑1 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa > 1:607855/1‑126 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa < 2:607855/126‑1 (MQ=255) ctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCt > 1:673680/1‑139 (MQ=255) tgatgaTTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGAACAg > 2:732308/1‑139 (MQ=255) tCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGgcgc > 2:523354/1‑75 (MQ=255) tCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGgcgc < 1:523354/75‑1 (MQ=255) ggTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATcag > 1:446791/1‑138 (MQ=255) gTCTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATcag > 1:322834/1‑137 (MQ=255) cTGTGCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCagcagc > 2:97415/1‑139 (MQ=255) tgCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCg < 1:955120/137‑1 (MQ=255) tgCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGt > 1:285184/1‑138 (MQ=255) tgCGGTGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGt > 2:1210907/1‑138 (MQ=255) tGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa > 2:716153/1‑80 (MQ=255) tGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGa < 1:716153/80‑1 (MQ=255) tGCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTg > 2:414044/1‑139 (MQ=255) tGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTg > 1:746185/1‑139 (MQ=255) gCTGGCGCGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTg > 1:405736/1‑138 (MQ=255) gcgcGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCt < 2:353356/109‑1 (MQ=255) gcgcGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCt > 1:353356/1‑109 (MQ=255) gcgcGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCAtt < 2:3090/138‑1 (MQ=255) gcgcGTCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCAt > 2:425448/1‑137 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAATCGGACGCTTCAGGGTCTGGACCAGTTGCGTCATCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCa > 1:1052929/1‑139 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGCTTTGCATTAGCTCa > 2:1180702/1‑139 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGAt > 1:686671/1‑126 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGAt < 2:686671/126‑1 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCa > 2:480399/1‑139 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCa > 2:1005258/1‑139 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCa > 1:890928/1‑139 (MQ=255) tCGATACAACCGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCa > 1:1125617/1‑139 (MQ=255) | GGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCA > NZ_CP009273/2521123‑2521378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |