Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	188,059	G→T	S239I (AGC→ATC)	tsf →	translation elongation factor Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	188,059	0	G	T	100.0%	231.4 / NA	58	S239I (AGC→ATC)	tsf	translation elongation factor Ts
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/28);  total (30/28)

AATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCA  >  NZ_CP009273/187924‑188188                                                                                                                                        |                                                                                                                                  aaTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCa                                                                                                                                 >  2:134105/1‑138 (MQ=255)            cAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGGCGTTCGTTATGGAACCAATCAAAACTGTTGGt                                                                                                                      <  2:330171/138‑1 (MQ=255)               cTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAgc                                                                                                                  >  1:197900/1‑139 (MQ=255)                   atatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAgctgct                                                                                                              >  2:606473/1‑139 (MQ=255)                    tatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGACGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAgctgct                                                                                                              >  2:313830/1‑138 (MQ=255)                    tatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAg                                                                                                                   <  1:139248/133‑1 (MQ=255)                    tatCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAg                                                                                                                   >  2:139248/1‑133 (MQ=255)                      tCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGa                                                                                                            <  2:753336/138‑1 (MQ=255)                       cgcgATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGa                                                                                                            >  1:1201747/1‑137 (MQ=255)                       cgcgATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGa                                                                                                            >  2:1182361/1‑137 (MQ=255)                        gcgATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGaaa                                                                                                          <  2:197900/138‑1 (MQ=255)                            tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCa                                                                                                     <  1:435203/139‑1 (MQ=255)                            tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCa                                                                                                     >  2:1205496/1‑139 (MQ=255)                            tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCa                                                                                                     <  1:500645/139‑1 (MQ=255)                            tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCa                                                                                                     <  1:319281/139‑1 (MQ=255)                            tGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCa                                                                                                     <  2:194556/139‑1 (MQ=255)                                 tCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACg                                                                                                <  1:1182361/139‑1 (MQ=255)                                 tCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACg                                                                                                >  1:927209/1‑139 (MQ=255)                                      tAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTg                                                                                             >  1:329946/1‑137 (MQ=255)                                      tAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTg                                                                                             >  1:850886/1‑137 (MQ=255)                                        aGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCa                                                                                                                    >  2:614596/1‑112 (MQ=255)                                        aGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCa                                                                                                                    <  1:614596/112‑1 (MQ=255)                                        aGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGt                                                                                         >  2:1023947/1‑139 (MQ=255)                                        aGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGt                                                                                         >  1:1086628/1‑139 (MQ=255)                                          ccGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGa                                                                                       >  2:972262/1‑139 (MQ=255)                                          ccGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGa                                                                                       <  2:329946/139‑1 (MQ=255)                                          ccGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGa                                                                                       <  1:1205496/139‑1 (MQ=255)                                           cGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGAc                                                                                      >  1:540124/1‑139 (MQ=255)                                               agaaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGc                                                                                  >  1:1183740/1‑139 (MQ=255)                                                    tCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGaaa                                                                                                          <  2:65846/110‑1 (MQ=255)                                                    tCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGaaa                                                                                                          >  1:65846/1‑110 (MQ=255)                                                            aaaaTGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGAc                                                                                      >  2:177335/1‑122 (MQ=255)                                                            aaaaTGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGAc                                                                                      <  1:177335/122‑1 (MQ=255)                                                              aaTGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGaa                                                                   <  2:130703/139‑1 (MQ=255)                                                                           cGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGa                                                                                       <  2:863139/106‑1 (MQ=255)                                                                           cGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGa                                                                                       >  1:863139/1‑106 (MQ=255)                                                                            gCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCAt                                                     <  2:850886/139‑1 (MQ=255)                                                                             cATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCAT‑ACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATc                                                    >  1:578787/1‑138 (MQ=255)                                                                                           ccGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAgac                                      >  1:950288/1‑139 (MQ=255)                                                                                                            aCCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAg                     <  1:134105/139‑1 (MQ=255)                                                                                                            aCCGGTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAg                     >  2:21337/1‑139 (MQ=255)                                                                                                                gTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGaa                                                                   >  1:680589/1‑89 (MQ=255)                                                                                                                gTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGaa                                                                   <  2:680589/89‑1 (MQ=255)                                                                                                                gTCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgc                 >  1:843116/1‑139 (MQ=255)                                                                                                                 tCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgct                >  1:976323/1‑139 (MQ=255)                                                                                                                 tCAGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgct                >  2:996491/1‑139 (MQ=255)                                                                                                                   aGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgctgc              <  2:1263093/139‑1 (MQ=255)                                                                                                                   aGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgctgc              <  1:972262/139‑1 (MQ=255)                                                                                                                   aGCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTtgctgc              <  1:21337/139‑1 (MQ=255)                                                                                                                    gCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCg             <  1:606473/139‑1 (MQ=255)                                                                                                                    gCCGTTCGTTATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCg             <  1:403834/139‑1 (MQ=255)                                                                                                                      cgttcgttATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGAt           >  1:985302/1‑139 (MQ=255)                                                                                                                          cgttATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGCAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTc        >  2:388056/1‑138 (MQ=255)                                                                                                                          cgttATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCg                                                   >  2:1226069/1‑95 (MQ=255)                                                                                                                          cgttATGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCg                                                   <  1:1226069/95‑1 (MQ=255)                                                                                                                              aTGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAg    <  2:950288/138‑1 (MQ=255)                                                                                                                              aTGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAg    <  2:976323/138‑1 (MQ=255)                                                                                                                               tGGAACCAATCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCa  <  1:111747/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                  AATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTTATGGAACCAAGCAAAACTGTTGGTCAGCTGCTGAAAGAGCATAACGCTGAAGTGACTGGCTTCATCCGCTTCGAAGTGGGTGAAGGCATCGAGAAAGTTGAGACTGACTTTGCAGCAGAAGTTGCTGCGATGTCCAAGCA  >  NZ_CP009273/187924‑188188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑