Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,575,962 T→C S59G (AGC→GGC)  ypfG ← DUF1176 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,575,9620TC89.2% 175.8 / 14.7 65S59G (AGC→GGC) ypfGDUF1176 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base C (20/38);  total (23/42)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

CTTGTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGG  >  NZ_CP009273/2575831‑2576094
                                                                                                                                   |                                                                                                                                    
cTTGTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCt                                                                                                                                >  1:1252339/1‑138 (MQ=255)
cTTGTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTc                                                                                                                               <  1:707213/139‑1 (MQ=255)
cTTGTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTc                                                                                                                               <  2:217148/139‑1 (MQ=255)
   gTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCa                                                                                                                              <  2:664849/137‑1 (MQ=255)
        cacTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcat                                                                                                                       <  2:15587/139‑1 (MQ=255)
          cTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcatca                                                                                                                     <  2:465487/139‑1 (MQ=255)
          cTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCAGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcatca                                                                                                                     <  1:9401/139‑1 (MQ=255)
          cTTAACGCCAACGGCTCGCCATCTAACAGCAACCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcatca                                                                                                                     >  1:414381/1‑139 (MQ=255)
             aaCGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAGGGTCATCAcc                                                                                                                   <  1:586774/138‑1 (MQ=255)
             aaCGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCAcc                                                                                                                   <  1:635612/138‑1 (MQ=255)
             aaCGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCAc                                                                                                                    <  2:422044/137‑1 (MQ=255)
             aaCGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCAc                                                                                                                    <  2:403953/137‑1 (MQ=255)
              aCGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAg                                                                                                                 <  1:956780/139‑1 (MQ=255)
                    aCGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCAt                                                                                                            >  1:949798/1‑138 (MQ=255)
                     cGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCa                                                                                                                              <  1:1210541/119‑1 (MQ=255)
                     cGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCa                                                                                                                              >  2:1210541/1‑119 (MQ=255)
                     cGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTa                                                                                                          >  1:398786/1‑139 (MQ=255)
                           gCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGAt                                                                                                      <  1:251605/137‑1 (MQ=255)
                            ccATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGc                                                                                                   <  2:398786/139‑1 (MQ=255)
                            ccATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGc                                                                                                   <  1:1100069/139‑1 (MQ=255)
                                      ggcgcCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTAcg                                                                                         >  2:1048222/4‑139 (MQ=255)
                                         gcCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCa                                                                                                             >  1:1010259/1‑116 (MQ=255)
                                         gcCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCa                                                                                                             <  2:1010259/116‑1 (MQ=255)
                                          ccGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACgcgc                                                                                      >  1:254672/1‑138 (MQ=255)
                                             tGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTc                                                                                                                               <  1:911788/94‑1 (MQ=255)
                                             tGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTc                                                                                                                               >  2:911788/1‑94 (MQ=255)
                                                         cccTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTg                                                                       <  1:533884/138‑1 (MQ=255)
                                                          ccTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGAGCCACGCAGAAATTTTGGt                                                                     <  2:949798/139‑1 (MQ=255)
                                                             tCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttat                                                                  >  2:223472/1‑139 (MQ=255)
                                                              cTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttat                                                                  <  2:341226/138‑1 (MQ=255)
                                                               tGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttatta                                                                >  2:934065/1‑139 (MQ=255)
                                                               tGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttatta                                                                >  1:1167810/1‑139 (MQ=255)
                                                                 aCGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAgg                                                                                                                            >  2:560323/1‑77 (MQ=255)
                                                                 aCGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAgg                                                                                                                            >  2:497572/1‑77 (MQ=255)
                                                                 aCGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAgg                                                                                                                            <  1:560323/77‑1 (MQ=255)
                                                                 aCGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAgg                                                                                                                            <  1:497572/77‑1 (MQ=255)
                                                                      tCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGa                                                         >  2:15418/1‑139 (MQ=255)
                                                                       ccGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGAc                                                        >  1:573937/1‑139 (MQ=255)
                                                                               ttCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtc                                                                                                                         <  1:1139069/66‑1 (MQ=255)
                                                                               ttCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtc                                                                                                                         >  2:1139069/1‑66 (MQ=255)
                                                                                 cAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGt                                                <  1:1001743/137‑1 (MQ=255)
                                                                                 cAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGCTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGt                                                <  1:1056992/137‑1 (MQ=255)
                                                                                      cgccgcGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGaaaa                                         <  2:174738/139‑1 (MQ=255)
                                                                                       gccgcGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAAc                                        <  2:1167810/139‑1 (MQ=255)
                                                                                                  tACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTgcgc                             <  1:984129/139‑1 (MQ=255)
                                                                                                  tACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTgcgc                             <  2:573937/139‑1 (MQ=255)
                                                                                                  tACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTgcgc                             <  1:941378/139‑1 (MQ=255)
                                                                                                  tACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTgcgc                             <  1:184453/139‑1 (MQ=255)
                                                                                                  tACGTAAAACGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTgcgc                             <  1:278636/139‑1 (MQ=255)
                                                                                                          aCGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttat                                                                  >  1:530878/1‑94 (MQ=255)
                                                                                                          aCGGCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGttat                                                                  <  2:530878/94‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  2:44460/139‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcat                                                                                                                       <  1:565199/39‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGtcat                                                                                                                       >  2:565199/1‑39 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCCCCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTAGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  2:414381/139‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  1:1241782/139‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  1:1048222/139‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  1:223472/139‑1 (MQ=255)
                                                                                                            ggCATCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTgccgcc                   <  2:663329/139‑1 (MQ=255)
                                                                                                               aTCGGTATGCGCCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCca                 >  2:123010/1‑138 (MQ=255)
                                                                                                                        cgcCCCGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGAc       <  1:756611/139‑1 (MQ=255)
                                                                                                                          ccccGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGt     <  1:580067/139‑1 (MQ=255)
                                                                                                                             cGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCCCACCAAAGACGCCgc  >  2:704385/1‑138 (MQ=255)
                                                                                                                             cGGCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCgg  >  1:948627/1‑139 (MQ=255)
                                                                                                                              ggCGCCGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCgg  >  1:486767/1‑138 (MQ=255)
                                                                                                                                   |                                                                                                                                    
CTTGTCACCACTTAACGCCAACGGCTCGCCATCTAACAGCAGCCGTGGCGCTATCTCCCCTTCTGACGCCTCCGGCGACTTCAATCCGCCGCGCTCAATACGTAAAACGGCATCGGTATGCGCCCCGGCGCTGCGGCTCAGGGTCATCACCAGTCCATTATGATCGCCCGTATTACGCGCCACGCAGAAATTTTGGTTATTACAGGTGACCTGCCAGTCGGAAAACGCCCGTTGCGCTGGTGCCGCCCACACCAAAGACGTCGG  >  NZ_CP009273/2575831‑2576094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑