Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,580,168	A→G	E360G (GAA→GGA)	narQ →	nitrate/nitrite two‑component system sensor histidine kinase NarQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,580,168	0	A	G	100.0%	125.0 / NA	33	E360G (GAA→GGA)	narQ	nitrate/nitrite two‑component system sensor histidine kinase NarQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GTTTACGGCGAACTGCACTGGCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAA  >  NZ_CP009273/2580032‑2580287                                                                                                                                         |                                                                                                                        gTTTACGGCGAACTGCACTGGCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGggag                                                                                                                       <  1:979879/139‑1 (MQ=255)    tACGGCGAACTGCACTGGCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGggagaa                                                                                                                     <  1:239399/138‑1 (MQ=255)                     gCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCcgc                                                                                                   >  2:784539/1‑139 (MQ=255)                               cACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCAc                                                                                          >  1:803323/1‑138 (MQ=255)                                          atcaGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCt                                                                              <  1:1094926/139‑1 (MQ=255)                                                    ctgctTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACttt                                                                    >  1:858246/1‑139 (MQ=255)                                                         tAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTcttac                                                               >  1:8602/1‑139 (MQ=255)                                                          aaCAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTctta                                                                <  1:408060/137‑1 (MQ=255)                                                               cGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACg                                                          >  1:142167/1‑138 (MQ=255)                                                               cGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACg                                                          >  2:578270/1‑138 (MQ=255)                                                                gTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTa                                                        <  1:1132798/139‑1 (MQ=255)                                                                         aTGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGGCTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                                               <  2:494940/139‑1 (MQ=255)                                                                         aTGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                                               <  1:176205/139‑1 (MQ=255)                                                                               ggACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTTCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCATTTGACGTTa                                          <  1:375170/138‑1 (MQ=255)                                                                                    cgGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTct                                                                  <  1:810405/109‑1 (MQ=255)                                                                                    cgGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTct                                                                  >  2:810405/1‑109 (MQ=255)                                                                                    cgGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                                               >  1:680619/1‑128 (MQ=255)                                                                                    cgGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGa                                               <  2:680619/128‑1 (MQ=255)                                                                                    cgGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAg                                    >  2:909533/1‑139 (MQ=255)                                                                                          gTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTc                               <  2:759343/138‑1 (MQ=255)                                                                                                tAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGaa                                     <  1:796318/126‑1 (MQ=255)                                                                                                tAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGaa                                     >  2:796318/1‑126 (MQ=255)                                                                                                 aaTCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATAc                          <  2:996608/136‑1 (MQ=255)                                                                                                 aaTCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATAc                          >  1:996608/1‑136 (MQ=255)                                                                                                 aaTCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCg                        >  2:1073152/1‑138 (MQ=255)                                                                                                      ggCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGaa                                     >  2:449526/1‑120 (MQ=255)                                                                                                      ggCGCAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGaa                                     <  1:449526/120‑1 (MQ=255)                                                                                                       gcgcAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGAt                  >  1:638491/1‑138 (MQ=255)                                                                                                        cgcAGAAGCATTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATaa                >  1:319009/1‑139 (MQ=255)                                                                                                                 aTTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTa                                                                        <  2:672228/74‑1 (MQ=255)                                                                                                                 aTTTTCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTa                                                                        >  1:672228/1‑74 (MQ=255)                                                                                                                  ttttCAGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGca      <  2:468838/139‑1 (MQ=255)                                                                                                                       aGCAATTATTGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACaaa  <  2:638491/138‑1 (MQ=255)                                                                                                                            ttattGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGc                                                                                   >  1:969240/1‑52 (MQ=255)                                                                                                                            ttattGTTGATGGGAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGc                                                                                   <  2:969240/52‑1 (MQ=255)                                                                                                                                         |                                                                                                                        GTTTACGGCGAACTGCACTGGCAAAATAGTCACGTTTCATCATCAGAACCGCTGCTTAACAGCGTTTCGTCGATGCTGGGACGCGGTTTGTACTTTAATCAGGCGCAGAAGCATTTTCAGCAATTATTGTTGATGGAAGAACGTGCGACCATCGCCCGCGAATTGCACGACTCGCTGGCTCAGGTACTTTCTTACTTACGTATCCAGTTGACGTTACTGAAGCGTTCGATACCGGAAGATAACGCCACCGCACAAA  >  NZ_CP009273/2580032‑2580287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑