| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,595,868 | T→C | L231S (TTG→TCG) | hyfB → | hydrogenase 4 subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,595,868 | 0 | T | C | 100.0% | 86.8 / NA | 26 | L231S (TTG→TCG) | hyfB | hydrogenase 4 subunit B |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (11/15); total (11/15) | |||||||||||
GCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTTGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCT > NZ_CP009273/2595734‑2595992 | gcgcGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCcg < 2:749959/139‑1 (MQ=255) gcgAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCcgcg > 1:330317/1‑139 (MQ=255) aaGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCt < 2:972101/139‑1 (MQ=255) gCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCa < 1:346763/139‑1 (MQ=255) ccTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCTTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGcc < 1:337149/139‑1 (MQ=255) aTTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCAc > 1:1003979/1‑138 (MQ=255) gCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCGGGTCGCCGCGCGCTCACCCTGCCGCACCATCg < 1:1111463/138‑1 (MQ=255) ccAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCa < 2:534182/139‑1 (MQ=255) cACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCg > 2:964707/1‑139 (MQ=255) tCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGAt < 2:330317/137‑1 (MQ=255) tcCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGt > 2:824169/1‑138 (MQ=255) tcCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGt > 2:299216/1‑138 (MQ=255) ggCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGt < 2:1032542/139‑1 (MQ=255) tgtTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTAtttt > 2:1038213/1‑139 (MQ=255) ttCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCg < 1:970368/139‑1 (MQ=255) gctgGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATc > 2:548561/1‑139 (MQ=255) ggCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCa > 1:755752/1‑66 (MQ=255) ggCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCa < 2:755752/66‑1 (MQ=255) ttCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAg > 2:658313/1‑139 (MQ=255) aaaGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGc < 2:261683/111‑1 (MQ=255) aaaGCCGGGATGATGCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGc > 1:261683/1‑111 (MQ=255) tgCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGt > 1:1155236/1‑109 (MQ=255) tgCCGTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGt < 2:1155236/109‑1 (MQ=255) gTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCct < 2:552699/139‑1 (MQ=255) gTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCct < 2:1003979/139‑1 (MQ=255) gTTGCACAGCTGGTCGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCct < 1:824169/139‑1 (MQ=255) | GCGCGAAAGCGGCAGCCTCGATTTTGCCAGTTTCCGCACGCTTTCACTTTCTCCGGGGCTGGCGTCGGCGGTGTTCCTGCTGGCCTTTTTCGGTTTTGGCGCGAAAGCCGGGATGATGCCGTTGCACAGCTGGTTGCCGCGCGCTCACCCTGCCGCACCATCGCACGCTTCGGCGTTGATGTCTGGCGTAATGGTCAAAATAGGTATTTTCGGCATCCTGAAAGTAGCGATGGATCTGCTGGCGCAAACGGGTTTGCCT > NZ_CP009273/2595734‑2595992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |