| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,631,737 | A→G | I92T (ATC→ACC) | bamB ← | outer membrane protein assembly factor BamB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,631,737 | 0 | A | G | 96.7% | 208.5 / ‑0.9 | 61 | I92T (ATC→ACC) | bamB | outer membrane protein assembly factor BamB |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (31/28); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCC > NZ_CP009273/2631604‑2631874 | ggTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCt > 1:166338/1‑139 (MQ=255) tATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCttt > 1:1219815/1‑139 (MQ=255) cAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGcc < 1:1020374/138‑1 (MQ=255) aGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCcat > 2:174937/1‑139 (MQ=255) cgcgTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCcatca > 2:1225191/1‑139 (MQ=255) cgTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCatcatc > 2:308155/1‑139 (MQ=255) cgcCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCa < 2:166338/137‑1 (MQ=255) gcCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAgcg > 2:645328/1‑139 (MQ=255) cTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCt < 2:1259504/139‑1 (MQ=255) cGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTa < 2:828465/138‑1 (MQ=255) gCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTAc < 2:221791/138‑1 (MQ=255) gACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg < 1:174937/138‑1 (MQ=255) aTGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTcc < 1:124339/139‑1 (MQ=255) aTGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTcc < 2:325280/139‑1 (MQ=255) tGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCg < 1:371509/139‑1 (MQ=255) tGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCg > 2:369852/1‑139 (MQ=255) gCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAgcgc > 2:524806/1‑115 (MQ=255) gCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAgcgc < 1:524806/115‑1 (MQ=255) cccACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAg > 1:263280/1‑111 (MQ=255) cccACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAg < 2:263280/111‑1 (MQ=255) cccACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCt > 2:1149833/1‑139 (MQ=255) caGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGATAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg > 1:713031/1‑127 (MQ=255) caGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGATAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg < 2:713031/127‑1 (MQ=255) caGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTcc > 1:1216053/1‑131 (MQ=255) caGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTcc < 2:1216053/131‑1 (MQ=255) caGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCa > 1:345868/1‑137 (MQ=255) gACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGa > 1:968816/1‑139 (MQ=255) acGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACaa < 1:1225191/139‑1 (MQ=255) ggTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGc < 2:1198309/129‑1 (MQ=255) ggTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGc > 1:1198309/1‑129 (MQ=255) gTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGt > 1:485387/1‑139 (MQ=255) gTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGt > 2:804450/1‑139 (MQ=255) cGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGc < 2:649031/139‑1 (MQ=255) ccAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGc > 2:465493/1‑137 (MQ=255) ggCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAt < 2:724867/139‑1 (MQ=255) ggCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAt < 2:485387/139‑1 (MQ=255) gCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAtt > 2:1211625/1‑139 (MQ=255) gCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAtt > 1:43395/1‑139 (MQ=255) tctTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTg > 1:585896/1‑138 (MQ=255) agaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGaa < 1:949399/139‑1 (MQ=255) gaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg > 1:692403/1‑84 (MQ=255) gaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg < 2:692403/84‑1 (MQ=255) gaACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAg > 2:64662/1‑139 (MQ=255) cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg > 1:490668/1‑80 (MQ=255) cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGcgcg < 2:490668/80‑1 (MQ=255) cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGc > 1:313729/1‑139 (MQ=255) cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGc > 1:1082587/1‑139 (MQ=255) cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGc > 1:103039/1‑139 (MQ=255) tctcGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCg < 2:1177104/137‑1 (MQ=255) ctcGGCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCt < 2:585896/138‑1 (MQ=255) gCCAGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCaa < 2:849070/139‑1 (MQ=255) aGGCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACgg < 1:308155/139‑1 (MQ=255) gCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCt < 2:521574/129‑1 (MQ=255) gCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCt > 1:521574/1‑129 (MQ=255) gCTGACAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACgg > 1:453612/1‑137 (MQ=255) aCAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAt < 2:543754/100‑1 (MQ=255) aCAGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGAt > 1:543754/1‑100 (MQ=255) aGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGc < 1:759688/81‑1 (MQ=255) aGACCAGGTTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGc > 2:759688/1‑81 (MQ=255) cAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACg > 1:740109/1‑139 (MQ=255) gATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGcc < 2:740109/139‑1 (MQ=255) | GGTATTCAGCGCGTAAACCTGCGCCTTTTCGCTGCCAATGTAGACATGCCCACCAGACACGGTCACACCGCCAGAAAGTAATGCAGGCTCTTTAGAGAACCAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCC > NZ_CP009273/2631604‑2631874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |