Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,636,307	A→G	intergenic (‑104/+181)	rodZ ← / ← rlmN	cytoskeleton protein RodZ/bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,636,307	0	A	G	100.0%	156.1 / NA	43	intergenic (‑104/+181)	rodZ/rlmN	cytoskeleton protein RodZ/bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/23);  total (20/23)

CTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCA  >  NZ_CP009273/2636170‑2636440                                                                                                                                          |                                                                                                                                      cTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGa                                                                                                                                       <  2:211388/138‑1 (MQ=255)  ttCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGcc                                                                                                                                     >  2:1249596/1‑139 (MQ=255)               tgcgtgGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAGACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCg                                                                                                                        >  1:428834/1‑139 (MQ=255)                   tgGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGAc                                                                                                                    <  1:1249596/139‑1 (MQ=255)                          aGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAGACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGcc                                                                                                              <  2:428834/138‑1 (MQ=255)                              ttcattcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca                                                                                                          <  1:132291/138‑1 (MQ=255)                              ttcattcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca                                                                                                          <  2:1111646/138‑1 (MQ=255)                              ttcattcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca                                                                                                          <  1:775058/138‑1 (MQ=255)                                 attcGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCa                                                                                                      >  2:952549/1‑139 (MQ=255)                                    cGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACt                                                                                                    <  2:910083/138‑1 (MQ=255)                                      cTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAg                                                                                                 >  1:89863/1‑139 (MQ=255)                                                 tttAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACt                                                                                      >  1:693760/1‑139 (MQ=255)                                                       tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCGCTAAGGCTAACTTACTGGTGCa                                                                                <  2:271918/139‑1 (MQ=255)                                                       tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa                                                                                <  2:212051/139‑1 (MQ=255)                                                       tGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa                                                                                <  2:693760/139‑1 (MQ=255)                                                          gaATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCg                                                                             >  1:346025/1‑139 (MQ=255)                                                                 gaTGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa                                                                      <  1:620945/139‑1 (MQ=255)                                                                                         cccACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACg                                               >  1:441884/1‑138 (MQ=255)                                                                                         cccACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGc                                              <  1:542481/139‑1 (MQ=255)                                                                                         cccACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGc                                              <  2:89863/139‑1 (MQ=255)                                                                                          ccACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCa                                             >  2:178211/1‑139 (MQ=255)                                                                                           cacaCCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCAc                                            <  1:1217587/139‑1 (MQ=255)                                                                                              acCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGt                                         <  2:441884/139‑1 (MQ=255)                                                                                              acCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGt                                         <  2:759583/139‑1 (MQ=255)                                                                                               ccGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCAccat                                         >  2:725676/1‑136 (MQ=255)                                                                                                  cgAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTg                                                                                  >  1:203991/1‑94 (MQ=255)                                                                                                  cgAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTg                                                                                  <  2:203991/94‑1 (MQ=255)                                                                                                  cgAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa                                      >  2:616881/1‑138 (MQ=255)                                                                                                  cgAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTaa                                      >  1:565524/1‑138 (MQ=255)                                                                                                        aTCCGTTAAGTTAACCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGAc                               <  2:565524/139‑1 (MQ=255)                                                                                                                  ttaaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTg                     >  1:756345/1‑139 (MQ=255)                                                                                                                  ttaaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTg                     <  1:688075/139‑1 (MQ=255)                                                                                                                    aaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca                                                                                                          <  1:1178293/52‑1 (MQ=255)                                                                                                                    aaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCcaca                                                                                                          >  2:1178293/1‑52 (MQ=255)                                                                                                                    aaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa                                                                      >  1:464085/1‑88 (MQ=255)                                                                                                                    aaCCGTTATCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa                                                                      <  2:464085/88‑1 (MQ=255)                                                                                                                           aTCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa                                                                                <  1:325371/71‑1 (MQ=255)                                                                                                                           aTCATACAGTATAAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCa                                                                                >  2:325371/1‑71 (MQ=255)                                                                                                                                    tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa                                                                      >  2:819055/1‑72 (MQ=255)                                                                                                                                    tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATa                                                                      <  1:819055/72‑1 (MQ=255)                                                                                                                                    tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAAt                       <  2:243880/119‑1 (MQ=255)                                                                                                                                    tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAAt                       >  1:243880/1‑119 (MQ=255)                                                                                                                                    tataAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACttcatt    >  1:1137472/1‑138 (MQ=255)                                                                                                                                      taAGGCCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACttcattca  >  1:1092075/1‑138 (MQ=255)                                                                                                                                          |                                                                                                                                      CTTCATTTTGGTCGTGCGTGGCTTCAGTATTCATTCGCTGCAGGTACGTTTAAATGAGAATTGAGATGCCGGTGAACCATCATGCTCACCCACACCGCGAAACATCCGTTAAGTTAACCGTTATCATACAGTATAAGACCGTCTGTGTGCTCGTGACAAAGCCCACATTCACTAAGGCTAACTTACTGTTGCATCGTTACATACTGCCTTAAAGTCAGCAAAAACGCACCGTTAATATTGACCAGACAATTGCAACTTAATACTTCATTCA  >  NZ_CP009273/2636170‑2636440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑