| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,667,151 | A→G | intergenic (+24/+24) | yphA → / ← yphB | DoxX family protein/aldose 1‑epimerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,667,151 | 0 | A | G | 95.2% | 188.5 / 2.1 | 62 | intergenic (+24/+24) | yphA/yphB | DoxX family protein/aldose 1‑epimerase |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (31/28); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.20e-01 | |||||||||||
CGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAT > NZ_CP009273/2667016‑2667285 | cGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAgcgc > 1:489474/1‑139 (MQ=255) atatGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTtaat < 2:920903/139‑1 (MQ=255) aTTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAAt > 1:51276/1‑138 (MQ=255) tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAg < 2:28428/138‑1 (MQ=255) tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAg > 1:28428/1‑138 (MQ=255) tttCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGc < 1:1207453/139‑1 (MQ=255) aGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAAc > 1:912999/1‑138 (MQ=255) gTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa < 1:124150/139‑1 (MQ=255) cGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtc > 1:204743/1‑139 (MQ=255) cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTtaat < 2:1081707/106‑1 (MQ=255) cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTtaat > 1:1081707/1‑106 (MQ=255) cTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACAtctc > 2:1264960/1‑139 (MQ=255) gcgcGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTg < 1:725641/138‑1 (MQ=255) cgcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGcc < 2:665695/86‑1 (MQ=255) cgcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGcc > 1:665695/1‑86 (MQ=255) gcgTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAg > 1:909840/1‑139 (MQ=255) ttCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAgttg < 1:77132/139‑1 (MQ=255) gCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGa < 1:913814/138‑1 (MQ=255) tGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcccc > 1:807479/1‑139 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa > 2:827973/1‑114 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGa < 1:827973/114‑1 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcccc < 1:203788/138‑1 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTcccc < 2:909840/138‑1 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt > 1:663217/1‑139 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt > 1:1051365/1‑139 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt > 2:411867/1‑139 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCt > 1:125579/1‑139 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc < 2:255450/71‑1 (MQ=255) ggCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGcc > 1:255450/1‑71 (MQ=255) aCCGGGCCGGGGGCAATTTCTCTCGATTGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCt < 2:835656/138‑1 (MQ=255) ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg < 1:1252789/135‑1 (MQ=255) ccgggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTg > 2:1252789/1‑135 (MQ=255) ggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcg > 2:1282772/1‑138 (MQ=255) ggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcg > 2:972520/1‑138 (MQ=255) ggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcg > 1:1040519/1‑138 (MQ=255) ggccgggGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcg > 1:765566/1‑138 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGCAGTTGTTGAtt > 2:141338/1‑118 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGAtt < 1:141338/118‑1 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc < 2:862604/134‑1 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAgcgc > 1:862604/1‑134 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt > 2:326425/1‑137 (MQ=255) ggggCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAAt > 2:30559/1‑137 (MQ=255) gCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGAt < 1:1006636/139‑1 (MQ=255) tctcGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCt > 2:474679/1‑139 (MQ=255) gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGcc < 2:974182/40‑1 (MQ=255) gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGcc > 1:974182/1‑40 (MQ=255) gATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCg < 1:494384/139‑1 (MQ=255) cGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCa < 1:60618/114‑1 (MQ=255) cGTTAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACACGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGATAGCGCa > 2:60618/1‑114 (MQ=255) ttAGGAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatga < 2:461601/139‑1 (MQ=255) ggAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatga < 2:125579/139‑1 (MQ=255) ggAAAGATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCgatgatg < 2:1051365/138‑1 (MQ=255) gATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatcatc > 2:456022/1‑139 (MQ=255) gATGCCGGATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGatcatc > 1:156152/1‑139 (MQ=255) ggATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGgcgc < 1:679116/139‑1 (MQ=255) gATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCa > 1:564016/1‑139 (MQ=255) gATGCGGCGTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCa < 1:474679/139‑1 (MQ=255) cggcgTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc > 1:223276/1‑139 (MQ=255) cggcgTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGc > 1:19805/1‑139 (MQ=255) cgTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCa > 2:976014/1‑139 (MQ=255) gTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt < 2:223276/139‑1 (MQ=255) gTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt < 2:1040519/139‑1 (MQ=255) gTGAGCGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAt < 1:976014/139‑1 (MQ=255) | CGATGCGGTTGGGCCAAATATGATTAATTTCTGGAAGAATGTCAGTATCGCTGGCGCGTTCTTGCTATTGGCAATTACCGGGCCGGGGGCAATTTCTCTCGATCGGCGTTAGGAAAGATGCCGGATGCGGCGTGAACGCCTTATCCGGCATTTAATAAATTACAGCCACTCTACCCGCAACGACATCTCTGAAGTTGTTGATTCCCCTGGTGCTAGCGCAATGAGATCACCCCCTTCCGGGCGATGATGATCATCCGGCGCATGGCTCAT > NZ_CP009273/2667016‑2667285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |