Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,716,348 A→G D88G (GAC→GGC)  pssA → CDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,716,3480AG94.4% 168.1 / 2.3 54D88G (GAC→GGC) pssACDP‑diacylglycerol‑‑serine O‑phosphatidyltransferase
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (27/24);  total (29/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

AAATAGCCAGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAG  >  NZ_CP009273/2716213‑2716482
                                                                                                                                       |                                                                                                                                      
aaaTAGCCAGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTg                                                                                                                                     <  2:169536/139‑1 (MQ=255)
     gCCAGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCAt                                                                                                                                 <  2:524006/138‑1 (MQ=255)
        aGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTg                                                                                                                             <  1:851998/139‑1 (MQ=255)
        aGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTg                                                                                                                             <  1:335196/139‑1 (MQ=255)
                gcagcGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGt                                                                                                                     <  2:696418/139‑1 (MQ=255)
                    cgcATTTGCATTGTCGCCTTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGAc                                                                                                                 >  1:165130/1‑139 (MQ=255)
                    cgcATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGAc                                                                                                                 >  2:601148/1‑139 (MQ=255)
                      cATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACg                                                                                                                >  2:407931/1‑138 (MQ=255)
                             aTTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGgcg                                                                                                        <  2:1238027/139‑1 (MQ=255)
                                 tCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGc                                                                                                    >  2:808781/1‑139 (MQ=255)
                                   gCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCgg                                                                                                  <  2:585319/139‑1 (MQ=255)
                                         tATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCt                                                                                             >  2:389052/1‑138 (MQ=255)
                                                aaCAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCt                                                                                             <  2:896418/131‑1 (MQ=255)
                                                aaCAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCt                                                                                             >  1:896418/1‑131 (MQ=255)
                                                   aGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGc                                                                                  >  1:773186/1‑139 (MQ=255)
                                                   aGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGc                                                                                  >  1:840821/1‑139 (MQ=255)
                                                   aGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGc                                                                                  >  1:1258990/1‑139 (MQ=255)
                                                   aGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGc                                                                                  >  2:1160972/1‑139 (MQ=255)
                                                     gATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCt                                                                                 >  2:8113/1‑138 (MQ=255)
                                                     gATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTg                                                                                <  1:407931/139‑1 (MQ=255)
                                                     gATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTg                                                                                <  2:206891/139‑1 (MQ=255)
                                                          cGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTgg                                                                           <  2:1258990/139‑1 (MQ=255)
                                                             tGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTAc                                                                        <  2:80864/139‑1 (MQ=255)
                                                             tGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTAc                                                                        >  2:949060/1‑139 (MQ=255)
                                                               gCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGcaca                                                                                                                         <  2:70973/88‑1 (MQ=255)
                                                               gCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGcaca                                                                                                                         >  1:70973/1‑88 (MQ=255)
                                                               gCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTg                                                                      >  2:1238449/1‑139 (MQ=255)
                                                               gCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTg                                                                      >  2:891262/1‑139 (MQ=255)
                                                               gCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTg                                                                      >  1:1242024/1‑139 (MQ=255)
                                                                    ggCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCa                                                                 <  2:1225083/139‑1 (MQ=255)
                                                                         tCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACg                                                                                   <  2:1061396/116‑1 (MQ=255)
                                                                         tCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACg                                                                                   >  1:1061396/1‑116 (MQ=255)
                                                                                   ttGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATc                                                  <  2:773186/139‑1 (MQ=255)
                                                                                            gCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCg                                            >  2:848073/1‑136 (MQ=255)
                                                                                              tAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGAt                                       >  1:293538/1‑139 (MQ=255)
                                                                                                aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCa                                                                                                                                  <  1:871602/46‑1 (MQ=255)
                                                                                                aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCa                                                                                                                                  >  2:871602/1‑46 (MQ=255)
                                                                                                aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCg                                                                                                   >  1:865299/1‑77 (MQ=255)
                                                                                                aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCg                                                                                                   <  2:865299/77‑1 (MQ=255)
                                                                                                aaaGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGt                                     >  1:1169655/1‑139 (MQ=255)
                                                                                                              gAACTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCGTTATGTTGATTCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTATTTTTTTCGGTTTATGGCg                       <  2:827777/139‑1 (MQ=255)
                                                                                                               aaCTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGt                      >  1:342032/1‑139 (MQ=255)
                                                                                                                aCTGGATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                     <  1:808781/139‑1 (MQ=255)
                                                                                                                    gATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTg                                                                                <  2:690113/76‑1 (MQ=255)
                                                                                                                    gATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTg                                                                                >  1:690113/1‑76 (MQ=255)
                                                                                                                    gATGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaa                 >  1:88016/1‑139 (MQ=255)
                                                                                                                     aTGTGCGGGTGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCcaat                <  1:891262/139‑1 (MQ=255)
                                                                                                                         gCGGGTGCTGGTCGGCTGGCATCGTGCAAAACGTGGACGCATTGGCGATGCGACATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTGATGGCGTTCcaatcaat            >  1:827777/1‑139 (MQ=255)
                                                                                                                              tGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                     <  2:1145106/125‑1 (MQ=255)
                                                                                                                              tGCTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGtt                     >  1:1145106/1‑125 (MQ=255)
                                                                                                                                cTGGTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTg     <  1:1010459/139‑1 (MQ=255)
                                                                                                                                cTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGt      >  2:297691/1‑138 (MQ=255)
                                                                                                                                   gTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGaa   <  2:1242024/138‑1 (MQ=255)
                                                                                                                                   gTCGGCTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAg  >  2:191013/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                      
AAATAGCCAGCGCGAAGCAGCGCATTTGCATTGTCGCCCTGTATCTCGAACAGGATGACGGTGGCAAAGGCATTCTGAACGCGTTGTATGAGGCTAAAAGGCAGCGTCCGGAACTGGATGTGCGGGTGCTGGTCGACTGGCATCGTGCACAACGTGGACGCATTGGCGCTGCGGCATCTAACACTAACGCTGACTGGTACTGCCGCATGGCGCAGGAAAATCCGGGCGTAGATGTTCCGGTTTATGGCGTTCCAATCAATACTCGTGAAG  >  NZ_CP009273/2716213‑2716482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑