| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,718,722 | A→G | L128L (CTT→CTC) | kgtP ← | alpha‑ketoglutarate permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,718,722 | 0 | A | G | 98.2% | 197.0 / ‑3.8 | 57 | L128L (CTT→CTC) | kgtP | alpha‑ketoglutarate permease |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (29/27); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-01 | |||||||||||
GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGC > NZ_CP009273/2718589‑2718855 | gTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCaa > 2:867648/1‑139 (MQ=255)gTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTATCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCaa > 2:232860/1‑139 (MQ=255) tCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCgg < 1:232860/139‑1 (MQ=255) tCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCa < 2:241424/139‑1 (MQ=255) gAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCta > 1:1265964/1‑138 (MQ=255) aaaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtat < 1:867648/138‑1 (MQ=255) aaaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtat < 1:586646/138‑1 (MQ=255) aaaTGATGCGTAAAAACCTTTGCGCCCTTCAACGGAAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCtata < 2:134397/139‑1 (MQ=255) tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa < 2:55339/139‑1 (MQ=255) tgCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa < 2:250196/139‑1 (MQ=255) cGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCAt < 1:891882/138‑1 (MQ=255) cGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAACCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCAt > 2:891882/1‑138 (MQ=255) tAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAAc > 1:1268373/1‑139 (MQ=255) ttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCt > 2:315785/1‑117 (MQ=255) ttGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCt < 1:315785/117‑1 (MQ=255) ttCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGAt > 2:227682/1‑138 (MQ=255) cGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg < 1:873704/139‑1 (MQ=255) gCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCg < 2:600374/139‑1 (MQ=255) aCTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATc > 1:852424/1‑139 (MQ=255) cACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGa < 1:1160668/138‑1 (MQ=255) aCTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa < 2:1268373/138‑1 (MQ=255) aCTCATATAGGTGGCGCTGGTGACATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa < 1:358161/138‑1 (MQ=255) ataGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg > 1:840806/1‑123 (MQ=255) ataGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg < 2:840806/123‑1 (MQ=255) taGGTGGCGCTGGGGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACAcatc < 2:259369/138‑1 (MQ=255) gTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac < 1:299942/139‑1 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAGCAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCa < 1:682248/75‑1 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAGCAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCa > 2:682248/1‑75 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa < 2:825641/91‑1 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCa > 1:825641/1‑91 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac < 2:859013/128‑1 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAac > 1:859013/1‑128 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca > 2:50334/1‑138 (MQ=255) ggCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATaca > 2:281742/1‑138 (MQ=255) gcgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacac < 1:269360/138‑1 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtata > 1:864187/1‑83 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtata < 2:864187/83‑1 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa < 1:275275/124‑1 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGaa > 2:275275/1‑124 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac > 2:76823/1‑139 (MQ=255) cgcTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATacacac > 1:431593/1‑139 (MQ=255) ggTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGa > 1:346858/1‑138 (MQ=255) gTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac < 2:824275/130‑1 (MQ=255) gTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATac > 1:824275/1‑130 (MQ=255) ccGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAtt > 1:69407/1‑139 (MQ=255) cgacAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCACAAGGACATAAAGTTTCATAACATGGGAGGCAGGCGATAACAAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAttt > 2:967290/4‑139 (MQ=255) cGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtata > 2:1063513/1‑65 (MQ=255) cGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCtata < 1:1063513/65‑1 (MQ=255) cGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAttt > 2:1166678/1‑139 (MQ=255) cGCCAACAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGAttt > 1:1187120/1‑139 (MQ=255) aCAGATAATCCCTGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGc > 2:151280/1‑139 (MQ=255) tAATCCCTGAAATAAACGAGCGATGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACTCATCATACACTCCGATAACAGCATCGATTTTTTGCGTCCa < 1:967290/139‑1 (MQ=255) tGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg > 2:154057/1‑80 (MQ=255) tGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAg < 1:154057/80‑1 (MQ=255) tGAAATAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTAt > 2:207176/1‑139 (MQ=255) aaTAAACGAGCGAGGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCgg < 1:281742/139‑1 (MQ=255) gcgagGAGCAATAATGCCGGAGCCCACGGACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGc > 2:370866/1‑139 (MQ=255) | GTCCGCCGATCAACGTCACATACTGAAATGATGCGTAAAAACCTTTGCGCCCTTCAACGGCAACTTCACTCATATAGGTGGCGCTGGTGCCATATTCTCCGCCAACAGATAATCCCTGAAATAAACGAGCGAGAAGCAATAATGCCGGAGCCCACGTACCTATAGTTTCATAACCTGGGAGGCAGGCGATAACCAGCGATCCGAAACACATCATACACACCGATAACAGCATCGATTTTTTGCGACCATGTTTATCGGCTATGCGGC > NZ_CP009273/2718589‑2718855 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |