| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,727,607 | A→G | intergenic (‑75/+55) | clpB ← / ← pgeF | ATP‑dependent chaperone ClpB/purine nucleoside phosphorylase YfiH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,727,607 | 0 | A | G | 97.7% | 159.3 / ‑4.0 | 44 | intergenic (‑75/+55) | clpB/pgeF | ATP‑dependent chaperone ClpB/purine nucleoside phosphorylase YfiH |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (19/24); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
TGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGT > NZ_CP009273/2727480‑2727739 | tGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAAtg > 1:162528/1‑139 (MQ=255) gCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCAc < 2:1263570/139‑1 (MQ=255) gCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCAc < 2:1151408/139‑1 (MQ=255) gCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCAc < 2:1096687/139‑1 (MQ=255) aGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCAcgcg > 2:82781/1‑139 (MQ=255) aTTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGa < 2:340103/139‑1 (MQ=255) aTTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGa < 2:1230186/139‑1 (MQ=255) aCGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCt < 1:1046831/139‑1 (MQ=255) aCGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCt < 1:938905/139‑1 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCtt < 2:927777/119‑1 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCtt > 1:927777/1‑119 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAg > 1:1019283/1‑139 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAg > 2:1274199/1‑139 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAg > 1:1075233/1‑139 (MQ=255) tAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAg > 1:1107413/1‑139 (MQ=255) aaCTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGc > 1:485478/1‑139 (MQ=255) aTAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGAt < 1:63891/102‑1 (MQ=255) aTAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGAt > 2:63891/1‑102 (MQ=255) aTCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTatat > 1:1007435/1‑118 (MQ=255) aTCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTatat < 2:1007435/118‑1 (MQ=255) aTCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCa > 2:693439/1‑138 (MQ=255) tCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAGGGTTATTGGCCAGATTAATGTGAAAAGAAATTCACGCGTACCGGATCGTCTTGATTCTTTAGGTTTTATAAGCCAAATGAAACTTGCCATa > 2:1205487/1‑139 (MQ=255) aGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGAc < 1:355154/139‑1 (MQ=255) aGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGAc < 1:372929/139‑1 (MQ=255) ttGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCg < 2:162528/139‑1 (MQ=255) cTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCggtg > 2:426858/1‑139 (MQ=255) cTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCggtggt < 2:351830/138‑1 (MQ=255) aTTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCtt > 2:1237268/1‑89 (MQ=255) aTTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCtt < 1:1237268/89‑1 (MQ=255) aTTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTcg > 1:1072118/1‑139 (MQ=255) ttAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTcgc < 2:824876/139‑1 (MQ=255) tAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTcgc > 1:736396/1‑138 (MQ=255) cccTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAgg > 1:1160574/1‑78 (MQ=255) cccTCAATTATTCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAgg < 2:1160574/78‑1 (MQ=255) ttCAAGGTTATTGGCCAGATTAATGTGAAATGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATaa > 2:155940/1‑123 (MQ=255) ttCAAGGTTATTGGCCAGATTAATGTGAAATGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATaa < 1:155940/123‑1 (MQ=255) ttCAAGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCAt > 2:581930/1‑139 (MQ=255) aGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTc < 2:311417/139‑1 (MQ=255) aGGTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTc < 1:581930/139‑1 (MQ=255) gTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg < 2:414780/139‑1 (MQ=255) gTTATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCg < 2:1019283/139‑1 (MQ=255) ttATTGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGt < 1:1175165/139‑1 (MQ=255) ttGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGAt > 1:312294/1‑41 (MQ=255) ttGGCCAGATTAATGTGAAAAGAAAATCACGCGTACCGGAt < 2:312294/41‑1 (MQ=255) | TGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCAGACGCATAACTCCTCCCATAACGGATCAAAGTTGCTACTGGAGATTAAATGAGGTCATCCCTCAATTATTCAAGGTTATTGACCAGATTAATGTGAAAAGAAAATCACGCGTACCGGATCGTCTTGATTCTTTAGGTTATATCAGCCAAATGAAACTTGCCATACGACCGGTGGTCTTGTCGCGACGATAAGAGAAGAAAGTCTCATTTTCCGT > NZ_CP009273/2727480‑2727739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |