| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,732,532 | A→G | D299D (GAT→GAC) | tyrA ← | bifunctional chorismate mutase/prephenate dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,732,532 | 0 | A | G | 97.9% | 163.4 / ‑3.7 | 47 | D299D (GAT→GAC) | tyrA | bifunctional chorismate mutase/prephenate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (25/21); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.68e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.59e-01 | |||||||||||
TGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGT > NZ_CP009273/2732396‑2732667 | tGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTc > 1:1183383/1‑139 (MQ=255)tGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTc > 2:308731/1‑139 (MQ=255) cGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCt > 2:865028/1‑139 (MQ=255) aaCGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGcc < 1:865028/139‑1 (MQ=255) tCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGc > 1:102823/1‑137 (MQ=255) cTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCa < 1:1288270/139‑1 (MQ=255) cTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCa < 1:983272/139‑1 (MQ=255) tGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTc > 2:1288270/1‑137 (MQ=255) gCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGcc > 2:180477/1‑120 (MQ=255) gCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGcc < 1:180477/120‑1 (MQ=255) cTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCaa < 2:433072/137‑1 (MQ=255) gCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGt > 1:689759/1‑138 (MQ=255) aaCTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTaaa < 1:308731/139‑1 (MQ=255) ctcgccGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAagag < 2:515817/139‑1 (MQ=255) taGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCt < 2:284837/139‑1 (MQ=255) aCGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGc > 1:1212530/1‑139 (MQ=255) aCGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGc > 2:727190/1‑139 (MQ=255) aCGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGc > 1:1200151/1‑139 (MQ=255) cGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCt > 1:621004/1‑139 (MQ=255) gTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCt > 2:774490/1‑138 (MQ=255) ttGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGaa > 1:913648/1‑139 (MQ=255) cGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttct > 1:968354/1‑139 (MQ=255) aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGcc < 1:807316/139‑1 (MQ=255) aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGcc < 1:774490/139‑1 (MQ=255) aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGcc < 2:1040035/139‑1 (MQ=255) aTTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGcc < 2:307425/139‑1 (MQ=255) tctGACGACATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTg > 1:441179/1‑137 (MQ=255) gacgacATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGcc > 1:991977/1‑139 (MQ=255) acgacATAATGATGTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGccc > 2:431492/1‑139 (MQ=255) atgatgTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCa > 2:140826/1‑138 (MQ=255) gatgTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCaa > 1:304401/1‑137 (MQ=255) tgTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttctt < 1:708394/111‑1 (MQ=255) tgTCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTttctt > 2:708394/1‑111 (MQ=255) tCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAg > 2:295210/1‑139 (MQ=255) tCGGCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAg > 1:987268/1‑139 (MQ=255) ggCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCa < 1:727190/139‑1 (MQ=255) ggCATAAAGCTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCa < 1:295210/139‑1 (MQ=255) cTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcgc < 2:1200151/139‑1 (MQ=255) cTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcgc < 2:437837/139‑1 (MQ=255) cTGCGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcgc < 1:325967/139‑1 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCa < 2:777257/127‑1 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCa > 1:777257/1‑127 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcg < 1:756926/135‑1 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGgcg > 2:756926/1‑135 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCa < 1:431492/137‑1 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCa < 2:621004/137‑1 (MQ=255) cGGGTCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGt > 2:267256/1‑139 (MQ=255) | TGCGGAAACTGTCAATAAACGCCTGCTTATCGCCCTGCTCCAGCAACTCAATCGCCTCGCCGAAACGCTTATAGTAACGTTTGATTAACGCCAGATTACGCTCTGACGACATAATGATGTCGGCATAAAGCTGCGGATCCTGAGCAAACAGTCGCCCGACCATCGCCAGCTCAAGGCGGTAAATCGGCGAAGAGAGCGCCAGAAGTTGCTCAAGCTGAACATTTTCTTCTGCCAGGTGCAGCCCGTAAGCAAAAGTAGCAAAGTGGCGCAGT > NZ_CP009273/2732396‑2732667 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |