| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,867,513 | A→G | D422D (GAT→GAC) | cysN ← | sulfate adenylyltransferase subunit CysN |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,867,513 | 0 | A | G | 96.9% | 222.2 / 0.1 | 66 | coding (1266/1428 nt) | cysN | sulfate adenylyltransferase subunit CysN |
| Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (26/37); minor base . (1/1); total (27/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGATCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTA > NZ_CP009273/2867380‑2867648 | cccAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGcggtcgat < 1:615811/139‑1 (MQ=255) ggAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAAt > 2:558192/1‑137 (MQ=255) aaaGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGc > 2:213481/1‑139 (MQ=255) gTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCa < 2:443065/139‑1 (MQ=255) tGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCAc < 2:462210/139‑1 (MQ=255) ggCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGcccccc > 2:1035701/1‑139 (MQ=255) ggCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCAcc > 2:51341/1‑139 (MQ=255) cgaACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCa < 1:1210938/139‑1 (MQ=255) cgaACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCG‑‑CGATAAAAATCAGCCCACCCGTCAcc > 2:326189/1‑139 (MQ=255) agCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCAc < 2:1257473/132‑1 (MQ=255) agCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCAc > 1:1257473/1‑132 (MQ=255) cATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTttg < 2:35976/139‑1 (MQ=255) aaTTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGAt < 1:1176417/139‑1 (MQ=255) ttCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa < 2:304843/139‑1 (MQ=255) gTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATc > 1:422019/1‑139 (MQ=255) ttCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCt < 1:527627/139‑1 (MQ=255) cAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGt < 1:1269400/138‑1 (MQ=255) gcgcAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGt > 2:85124/1‑139 (MQ=255) gCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCa > 2:376778/1‑123 (MQ=255) gCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCa < 1:376778/123‑1 (MQ=255) gCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCAc > 2:1205643/1‑137 (MQ=255) gCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGCGAGATCCAc > 1:577655/1‑137 (MQ=255) tGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa > 1:565078/1‑81 (MQ=255) tGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa < 2:565078/81‑1 (MQ=255) cACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGc < 1:596632/139‑1 (MQ=255) cACCATACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGc < 2:471141/139‑1 (MQ=255) tACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTgag > 1:106071/1‑106 (MQ=255) tACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTgag < 2:106071/106‑1 (MQ=255) tACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc < 1:1106436/117‑1 (MQ=255) tACCGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc > 2:1106436/1‑117 (MQ=255) ccGGCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGtttt < 2:929880/139‑1 (MQ=255) gCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa > 2:91737/1‑69 (MQ=255) gCACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa < 1:91737/69‑1 (MQ=255) cACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa > 2:908156/1‑68 (MQ=255) cACCCACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa < 1:908156/68‑1 (MQ=255) cccACGGTCACATTGCTCAGGCGGTCGATAAATATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCg > 1:1124849/1‑111 (MQ=255) cccACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCAc < 1:1091993/104‑1 (MQ=255) cccACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCAc > 2:1091993/1‑104 (MQ=255) cccACGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCg < 2:1124849/111‑1 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc > 1:625210/1‑139 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc < 2:625210/139‑1 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc > 1:811089/1‑139 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc < 1:567326/139‑1 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc < 1:51341/139‑1 (MQ=255) aCGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCAc < 1:1205643/139‑1 (MQ=255) cGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACg < 1:85124/139‑1 (MQ=255) cGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACg < 1:360891/139‑1 (MQ=255) cGGTCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACg < 2:395136/139‑1 (MQ=255) tCACATTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTg > 2:291573/1‑139 (MQ=255) acaTTGCTCAGGCG‑‑CGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGt < 1:326189/138‑1 (MQ=255) caTTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGt < 2:773773/139‑1 (MQ=255) caTTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGt < 2:877302/139‑1 (MQ=255) caTTGCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGt < 2:422019/139‑1 (MQ=255) caTTGCTCAGGCGATCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGt < 2:984913/139‑1 (MQ=255) gCTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAg < 1:213481/138‑1 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc < 1:1048316/94‑1 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc > 2:1122970/1‑94 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc > 2:1048316/1‑94 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGc < 1:1122970/94‑1 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACg > 1:967737/1‑128 (MQ=255) cTCAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACg < 2:967737/128‑1 (MQ=255) cAGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGtta > 1:1021631/1‑139 (MQ=255) aGGCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGttat < 1:291573/139‑1 (MQ=255) ggCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa > 2:526772/1‑47 (MQ=255) ggCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATaa < 1:526772/47‑1 (MQ=255) ggCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGttat > 1:264067/1‑138 (MQ=255) gCGGTCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGttatta < 2:1022341/139‑1 (MQ=255) atcgatAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCAc < 2:24822/81‑1 (MQ=255) atcgatAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCAc > 1:24822/1‑81 (MQ=255) | CCCAGTGCGGAAAGTGGCGACGAACCAGAGCATTCAATTCCAGTTCGAATGCACTGAATTCAGATGGCGCAGCAGTTGCCTGGCTAACTGGCTCGTGCACCATACCGGCACCCACGGTCACATTGCTCAGGCGATCGATAAAAATCAGCCCACCCGTCACCGGATTTTGTTGATAACGATCTAACACCAGCGGCTCGTCAAAAGTGAGATCCACGAGGCCGATCCCATTCAGTGGCAGGTTTTCAACTTCACGCTGGGTAAGGTTATTA > NZ_CP009273/2867380‑2867648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |