| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,901,362 | A→G | intergenic (‑62/+26) | eno ← / ← pyrG | phosphopyruvate hydratase/CTP synthase (glutamine hydrolyzing) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,901,362 | 0 | A | G | 96.0% | 165.7 / ‑1.1 | 50 | intergenic (‑62/+26) | eno/pyrG | phosphopyruvate hydratase/CTP synthase (glutamine hydrolyzing) |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (20/28); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01 | |||||||||||
TACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCA > NZ_CP009273/2901226‑2901497 | tACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGcg > 1:954506/1‑139 (MQ=255) aCTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTgcgc > 2:541227/1‑139 (MQ=255) cTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAGGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgcg > 2:645012/1‑139 (MQ=255) tCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGtt > 2:1056643/1‑139 (MQ=255) tCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGct > 1:582296/1‑139 (MQ=255) tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACtt > 1:1067709/1‑139 (MQ=255) tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACtt > 1:1101051/1‑139 (MQ=255) tCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACtt > 2:751881/1‑139 (MQ=255) gAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCg > 1:477168/1‑139 (MQ=255) atgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGAc < 1:541227/139‑1 (MQ=255) ttttGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTca > 1:36622/1‑138 (MQ=255) tGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACaa < 1:369056/138‑1 (MQ=255) gACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGc < 2:1067709/139‑1 (MQ=255) aTTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg < 2:1013728/139‑1 (MQ=255) ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCaaa < 1:1286596/139‑1 (MQ=255) ttCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAg < 1:1095655/138‑1 (MQ=255) cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg < 2:36622/139‑1 (MQ=255) aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGa < 1:816872/139‑1 (MQ=255) gTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc > 2:1237480/1‑138 (MQ=255) gTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc > 1:907287/1‑138 (MQ=255) aCTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATc < 2:601368/139‑1 (MQ=255) tAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc > 2:58999/1‑139 (MQ=255) gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg < 1:58999/138‑1 (MQ=255) gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg < 2:102461/138‑1 (MQ=255) gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg < 2:1101051/138‑1 (MQ=255) gTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg < 2:474956/138‑1 (MQ=255) aaaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg < 1:587347/98‑1 (MQ=255) aaaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg > 2:587347/1‑98 (MQ=255) aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg < 1:1284182/139‑1 (MQ=255) aaCTGAAACTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg < 1:228403/139‑1 (MQ=255) aCTCCAGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAg > 1:168885/1‑138 (MQ=255) aGACAAACAACGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACt < 2:907287/139‑1 (MQ=255) aacaacGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa < 1:1237480/139‑1 (MQ=255) aacaacGCGTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGa < 2:35443/139‑1 (MQ=255) cgTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg < 2:236235/88‑1 (MQ=255) cgTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg > 1:236235/1‑88 (MQ=255) cgTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg > 1:482746/1‑110 (MQ=255) cgTACCCAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg < 2:482746/110‑1 (MQ=255) cccAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGc < 2:582296/138‑1 (MQ=255) cccAGGGTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGc < 1:243102/138‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcggc > 2:381894/1‑65 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcggc < 1:381894/65‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg > 2:642384/1‑89 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCgg < 1:642384/89‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg < 1:904905/103‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTg > 2:904905/1‑103 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg > 2:564970/1‑106 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg < 1:564970/106‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCa < 2:1221460/139‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCa < 2:602844/139‑1 (MQ=255) ggTGCGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCa > 1:399251/1‑139 (MQ=255) | TACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCA > NZ_CP009273/2901226‑2901497 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |