Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,909,267 G→A K284K (AAG→AAA)  barA → two‑component sensor histidine kinase BarA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,909,2670GA94.2% 245.2 / 2.5 69K284K (AAG→AAA) barAtwo‑component sensor histidine kinase BarA
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base A (31/34);  total (33/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

GTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAACACCAACGCAGCGCGAT  >  NZ_CP009273/2909131‑2909402
                                                                                                                                        |                                                                                                                                       
gTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAacg                                                                                                                                       <  1:247144/139‑1 (MQ=255)
   tCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAacgcgc                                                                                                                                    >  2:914307/1‑139 (MQ=255)
           aTGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAg                                                                                                                            >  2:649693/1‑139 (MQ=255)
           aTGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAg                                                                                                                            >  2:770405/1‑139 (MQ=255)
                aaTGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAgcggcg                                                                                                                       <  1:770405/139‑1 (MQ=255)
                  tGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGcgcg                                                                                                                     >  1:762407/1‑139 (MQ=255)
                        tggctgCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTAtt                                                                                                                 <  1:648592/137‑1 (MQ=255)
                        tggctgCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTAtt                                                                                                                 >  2:648592/1‑137 (MQ=255)
                                  tCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGttt                                                                                                     <  1:1113882/139‑1 (MQ=255)
                                        agagATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGc                                                                                                >  1:236146/1‑138 (MQ=255)
                                             tGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAatat                                                                                          <  2:616893/139‑1 (MQ=255)
                                             tGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAatat                                                                                          <  2:588432/139‑1 (MQ=255)
                                                  cacaATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTca                                                                                      >  2:96815/1‑138 (MQ=255)
                                                    caATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCacac                                                                                   <  1:649693/139‑1 (MQ=255)
                                                       tatCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  2:47083/139‑1 (MQ=255)
                                                       tatCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  2:290191/139‑1 (MQ=255)
                                                       tatCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  1:833442/139‑1 (MQ=255)
                                                         tCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCt                                                                              <  2:997438/139‑1 (MQ=255)
                                                            aCCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                >  2:806980/1‑134 (MQ=255)
                                                            aCCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  1:806980/134‑1 (MQ=255)
                                                            aCCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCg                                                                           >  1:1183011/1‑139 (MQ=255)
                                                            aCCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCg                                                                           >  2:1205542/1‑139 (MQ=255)
                                                              cAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  2:58789/139‑1 (MQ=255)
                                                              cAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  1:25329/139‑1 (MQ=255)
                                                              cAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  1:914307/139‑1 (MQ=255)
                                                              cAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  2:1197006/139‑1 (MQ=255)
                                                                ggCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTaca                                                                       <  2:611118/139‑1 (MQ=255)
                                                                ggCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTaca                                                                       >  2:276018/1‑139 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTa                                                                                                                   >  1:123263/1‑94 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTa                                                                                                                   <  2:123263/94‑1 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTacac                                                                      >  2:430371/1‑139 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTacac                                                                      >  1:892730/1‑139 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  2:1157395/136‑1 (MQ=255)
                                                                 gCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         >  1:1157395/1‑136 (MQ=255)
                                                                   gACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTAcacca                                                                    <  1:458675/139‑1 (MQ=255)
                                                                      gTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACt                                                                  >  1:870484/1‑138 (MQ=255)
                                                                           aTCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  2:518862/119‑1 (MQ=255)
                                                                           aTCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                >  1:518862/1‑119 (MQ=255)
                                                                           aTCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         >  1:164480/1‑126 (MQ=255)
                                                                           aTCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  2:164480/126‑1 (MQ=255)
                                                                           aTCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATgg                                                            >  2:1077908/1‑139 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                <  2:1000898/114‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAg                                                                                >  1:1000898/1‑114 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGc                                                                               <  1:58921/115‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGc                                                                               >  2:58921/1‑115 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         >  1:509749/1‑121 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTa                                                                         <  2:509749/121‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTg                                                                 <  2:346198/129‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGaa                                                               <  2:351295/131‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGaa                                                               <  1:711154/131‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATg                                                             <  2:762407/133‑1 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTa                                                       >  2:234525/1‑139 (MQ=255)
                                                                                cGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTa                                                       >  1:1200042/1‑139 (MQ=255)
                                                                                                  aTGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGa                                     >  1:662485/1‑139 (MQ=255)
                                                                                                  aTGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGa                                     >  2:899999/1‑139 (MQ=255)
                                                                                                  aTGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGa                                     >  2:1262883/1‑139 (MQ=255)
                                                                                                   tGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGAc                                    >  2:250712/1‑139 (MQ=255)
                                                                                                      aaaTTCAGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCt                                 >  1:761559/1‑139 (MQ=255)
                                                                                                            aGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTc                                                                                                    >  1:638941/1‑66 (MQ=255)
                                                                                                            aGAACGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTc                                                                                                    <  2:638941/66‑1 (MQ=255)
                                                                                                                cGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCt                                                                              >  1:842447/1‑84 (MQ=255)
                                                                                                                cGTTGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCt                                                                              <  2:842447/84‑1 (MQ=255)
                                                                                                                  ttGAGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAAtt                     <  2:892730/139‑1 (MQ=255)
                                                                                                                     aGTTAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTCATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTaa                   <  2:1178533/138‑1 (MQ=255)
                                                                                                                       ttAGATCTGGCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAacac                <  1:73533/139‑1 (MQ=255)
                                                                                                                               ggCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTaca                                                                       >  2:565094/1‑76 (MQ=255)
                                                                                                                               ggCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTaca                                                                       <  1:565094/76‑1 (MQ=255)
                                                                                                                               ggCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGaa                                                               >  1:1013616/1‑84 (MQ=255)
                                                                                                                               ggCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGaa                                                               <  2:1013616/84‑1 (MQ=255)
                                                                                                                               ggCGAAAAAACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAACACCAACGCAg        >  1:1143149/1‑139 (MQ=255)
                                                                                                                                    aaaaACGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAACACCAACGCAGCGCGa   <  2:756975/139‑1 (MQ=255)
                                                                                                                                     aaaaCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAACACCAACGCAGCGCGAt  <  1:96815/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GTATCAACTCGATGGCAATGTCGCTGGCTGCTTATCACGAAGAGATGCAGCACAATATCGACCAGGCGACGTCCGATCTGCGTGAAACGCTGGAGCAGATGGAAATTCAGAACGTTGAGTTAGATCTGGCGAAAAAGCGCGCCCAGGAAGCGGCGCGTATTAAATCCGAGTTTCTGGCAAATATGTCACACGAGCTGCGTACACCACTGAATGGTGTTATTGGCTTTACCCGCCTGACGCTGAAAACAGAATTAACACCAACGCAGCGCGAT  >  NZ_CP009273/2909131‑2909402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑