| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,972,598 | A→G | R73R (CGA→CGG) | lysR → | DNA‑binding transcriptional regulator LysR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,972,598 | 0 | A | G | 98.2% | 189.2 / ‑3.8 | 55 | R73R (CGA→CGG) | lysR | DNA‑binding transcriptional regulator LysR |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (32/22); total (32/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.26e-01 | |||||||||||
CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTGGC > NZ_CP009273/2972464‑2972732 | cacacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTCCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc < 1:247261/137‑1 (MQ=255)cacacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTcc < 1:114157/138‑1 (MQ=255)cacacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc < 2:135694/137‑1 (MQ=255)cacacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc < 2:1048417/137‑1 (MQ=255) cacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTgg > 2:562284/1‑139 (MQ=255) cccgcccaGCCAACCGTCAGACGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGt < 2:653488/133‑1 (MQ=255) ccTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc < 2:279484/133‑1 (MQ=255) ccTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc > 1:279484/1‑133 (MQ=255) ccTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTa > 1:568527/1‑139 (MQ=255) cacaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACg > 2:589904/1‑138 (MQ=255) ccAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTgg < 1:1084014/139‑1 (MQ=255) ccAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTgg < 1:589904/139‑1 (MQ=255) aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc > 2:810677/1‑122 (MQ=255) aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTc < 1:810677/122‑1 (MQ=255) aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTa < 1:841576/128‑1 (MQ=255) aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTa > 2:841576/1‑128 (MQ=255) aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATc > 2:251790/1‑139 (MQ=255) gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGt > 1:1228225/1‑139 (MQ=255) cgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAgc > 1:1046124/1‑139 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgcgc < 1:844840/134‑1 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgcgc > 2:844840/1‑134 (MQ=255) tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGtt > 1:329883/1‑139 (MQ=255) gggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGcc < 1:562284/139‑1 (MQ=255) aaaTTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGga < 1:837790/138‑1 (MQ=255) ttGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAAc < 2:329355/139‑1 (MQ=255) ttGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTATGTTTGAAGAAGTGCAACGGTCCTGGTACGGGCTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAAc < 2:1046124/139‑1 (MQ=255) aGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGt < 2:837200/72‑1 (MQ=255) aGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGt > 1:837200/1‑72 (MQ=255) aGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTAt > 2:1086890/1‑139 (MQ=255) tACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATtgcctg > 2:375416/1‑139 (MQ=255) aTTACATCCTAACGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTc > 2:670338/1‑139 (MQ=255) cTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCa < 1:1086890/139‑1 (MQ=255) ccGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGcc < 1:511325/96‑1 (MQ=255) ccGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGcc > 2:511325/1‑96 (MQ=255) ccGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATc > 2:804354/1‑139 (MQ=255) ccGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATc > 2:40162/1‑139 (MQ=255) cGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCt > 2:489514/1‑139 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCg > 1:507132/1‑91 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCg < 2:507132/91‑1 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCttt > 2:827167/1‑139 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCttt > 2:990272/1‑139 (MQ=255) tGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCttt > 2:318764/1‑139 (MQ=255) aaGGACTGCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCttttt > 2:1164188/1‑138 (MQ=255) gCGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAg > 1:540059/1‑139 (MQ=255) cGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAg < 1:990272/138‑1 (MQ=255) cGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGc > 2:32458/1‑139 (MQ=255) cGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGc > 2:769597/1‑139 (MQ=255) cGTCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGc > 1:228071/1‑139 (MQ=255) tCTGTTTGAAGAAGTGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTc > 2:1155211/1‑139 (MQ=255) tGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCt > 2:1238533/1‑139 (MQ=255) tGCAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCt > 2:863577/1‑139 (MQ=255) cAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTgg < 1:251790/139‑1 (MQ=255) cAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTgg > 2:916397/1‑139 (MQ=255) cAACGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGAAACCCTTTCTg < 1:863577/138‑1 (MQ=255) aaCGGTCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTGGc < 1:32458/139‑1 (MQ=255) | CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAGTTTCGCCAGGGAGAACTGTCTATTGCCTGCCTGCCGGTCTTTTCGCAATCTTTTTTACCGCAGCTCCTGCAACCCTTTCTGGC > NZ_CP009273/2972464‑2972732 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |