Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,985,850 A→G D133G (GAT→GGT)  ygeH → HilA family transcriptional regulator YgeH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,985,8500AG100.0% 139.8 / NA 38D133G (GAT→GGT) ygeHHilA family transcriptional regulator YgeH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/11);  total (27/11)

AAACAATCTACCGGAAAGGTTATCGTTTCAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGATCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTC  >  NZ_CP009273/2985727‑2985983
                                                                                                                           |                                                                                                                                     
aaaCAATCTACCGGAAAGGTTATCGTTTCAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCAtt                                                                                                                                 <  1:282568/130‑1 (MQ=255)
aaaCAATCTACCGGAAAGGTTATCGTTTCAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCAtt                                                                                                                                 >  2:282568/1‑130 (MQ=255)
                            cAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCaaa                                                                                             >  2:1008602/1‑138 (MQ=255)
                            cAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCaaa                                                                                             >  1:710236/1‑138 (MQ=255)
                                         ttCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGAt                                                                                <  2:1053008/138‑1 (MQ=255)
                                          tCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATg                                                                               <  2:536587/138‑1 (MQ=255)
                                          tCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATg                                                                               <  1:435862/138‑1 (MQ=255)
                                                                        cAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACaaa                                                >  2:631102/1‑139 (MQ=255)
                                                                        cAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACaaa                                                >  1:355909/1‑139 (MQ=255)
                                                                        cAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACa                                                  <  2:710236/137‑1 (MQ=255)
                                                                         aGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAAt                                               >  1:294385/1‑139 (MQ=255)
                                                                               ttCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTg                                          >  1:899105/1‑138 (MQ=255)
                                                                                    tAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGa                                    <  2:899105/139‑1 (MQ=255)
                                                                                     aGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGAt                                                                                >  1:1273188/1‑94 (MQ=255)
                                                                                     aGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGAt                                                                                <  2:1273188/94‑1 (MQ=255)
                                                                                     aGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTa                                        <  1:419292/134‑1 (MQ=255)
                                                                                     aGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTa                                        >  2:419292/1‑134 (MQ=255)
                                                                                     aGCTATATTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGAt                                   >  1:778318/1‑139 (MQ=255)
                                                                                         atatTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTa                                        >  2:971467/1‑130 (MQ=255)
                                                                                         atatTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTa                                        <  1:971467/130‑1 (MQ=255)
                                                                                         atatTCCCTTTCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATcaca                               >  2:479963/1‑139 (MQ=255)
                                                                                                 tttCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGCATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCa                        >  2:603755/1‑138 (MQ=255)
                                                                                                 tttCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCa                        >  2:1091526/1‑138 (MQ=255)
                                                                                                 tttCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCa                        >  1:684707/1‑138 (MQ=255)
                                                                                                 tttCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCa                        >  2:1116561/1‑138 (MQ=255)
                                                                                                 tttCACTACTTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCa                        >  1:64239/1‑138 (MQ=255)
                                                                                                      ctactTCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAatt                   >  2:627224/1‑138 (MQ=255)
                                                                                                           tCATTGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCt             >  2:798555/1‑139 (MQ=255)
                                                                                                               tGAATACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAg         >  2:309725/1‑139 (MQ=255)
                                                                                                                 aaTACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATg                                                                               >  2:1275779/1‑67 (MQ=255)
                                                                                                                 aaTACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATg                                                                               <  1:1275779/67‑1 (MQ=255)
                                                                                                                 aaTACACTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAg         >  1:519119/1‑137 (MQ=255)
                                                                                                                      acTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATAc                                                                     >  2:862383/1‑72 (MQ=255)
                                                                                                                      acTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATAc                                                                     <  1:862383/72‑1 (MQ=255)
                                                                                                                      acTGGGTCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGAtt   >  1:468832/1‑138 (MQ=255)
                                                                                                                       cTGGGTCCATTAATACTTAATCA‑GAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTc  >  1:336146/1‑137 (MQ=255)
                                                                                                                       cTGGGTCCATTAATACTTAATCA‑GAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTc  >  1:1169209/1‑137 (MQ=255)
                                                                                                                        tGGGTCCATTAATACCTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTc  >  2:1181395/1‑137 (MQ=255)
                                                                                                                           |                                                                                                                                     
AAACAATCTACCGGAAAGGTTATCGTTTCAGCGGGCAGGTTTTCAAAACTAAAATTAATGAAGATAATACTTCAGACTATTCCATAGCTATATTCCCTTTCACTACTTCATTGAATACACTGGATCCATTAATACTTAATCAGGAATTAGTGCAAATCATTTCAAATAAAAAAATCGATGGTCTCTATACCTATCCGATGGCTGCGACAAATTTTTGTAATGATCACATATCTCAAAATTCATTCTTGAGCAGATTC  >  NZ_CP009273/2985727‑2985983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑