| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,999,490 | T→C | intergenic (‑345/‑134) | ygeV ← / → ygeW | sigma‑54‑dependent Fis family transcriptional regulator/knotted carbamoyltransferase YgeW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,999,490 | 0 | T | C | 97.1% | 234.7 / ‑1.1 | 68 | intergenic (‑345/‑134) | ygeV/ygeW | sigma‑54‑dependent Fis family transcriptional regulator/knotted carbamoyltransferase YgeW |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (29/37); total (30/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
CCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAA > NZ_CP009273/2999355‑2999628 | ccccTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCtt > 2:885395/1‑139 (MQ=255) tATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAg > 2:187350/1‑139 (MQ=255) aTTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCcgcgc > 2:810660/1‑139 (MQ=255) aTTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCcgc < 1:932228/137‑1 (MQ=255) tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCcgcgcg < 2:242821/139‑1 (MQ=255) tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCcgcgcg > 2:896665/1‑139 (MQ=255) tttCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCcgcgcg > 2:504285/1‑139 (MQ=255) gATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATaa > 2:1021144/1‑139 (MQ=255) aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGAt < 2:1050789/136‑1 (MQ=255) aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGAt < 1:261629/136‑1 (MQ=255) aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATaa < 2:738926/138‑1 (MQ=255) aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATaa < 2:266120/138‑1 (MQ=255) aTACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATaa < 2:343082/138‑1 (MQ=255) agcagcAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTgg > 2:379654/1‑138 (MQ=255) agcagcAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTgg > 2:1002649/1‑138 (MQ=255) agcagcAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTgg > 1:930126/1‑138 (MQ=255) gcagcaAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca < 2:337068/139‑1 (MQ=255) gcagcaAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca < 2:84275/139‑1 (MQ=255) gcagcaAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca < 2:1253595/139‑1 (MQ=255) gcagcaAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca < 1:1103050/139‑1 (MQ=255) caAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACt > 2:327010/1‑139 (MQ=255) aataaataACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTa < 1:596558/139‑1 (MQ=255) cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 2:114778/139‑1 (MQ=255) cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 1:327010/139‑1 (MQ=255) cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 2:824549/139‑1 (MQ=255) cgcgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 1:187350/139‑1 (MQ=255) cgATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACACCCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTccc < 1:524014/139‑1 (MQ=255) aTAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAg > 2:121716/1‑139 (MQ=255) aTAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAg > 2:991660/1‑139 (MQ=255) aTAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAg > 1:383518/1‑139 (MQ=255) tAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGc > 1:575363/1‑118 (MQ=255) tAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGc < 2:575363/118‑1 (MQ=255) aaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGc < 1:810660/139‑1 (MQ=255) aaGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGc < 2:668453/139‑1 (MQ=255) gTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAt < 1:744624/139‑1 (MQ=255) ttACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACt < 1:765700/97‑1 (MQ=255) ttACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACt > 2:765700/1‑97 (MQ=255) tACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTa > 1:741119/1‑104 (MQ=255) tACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTa < 2:741119/104‑1 (MQ=255) ccAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTca < 1:504285/139‑1 (MQ=255) aGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTcaca < 1:379654/139‑1 (MQ=255) aGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTcaca < 1:247516/139‑1 (MQ=255) gAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg < 1:1002649/139‑1 (MQ=255) gAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAg < 1:121716/139‑1 (MQ=255) gCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 1:460859/92‑1 (MQ=255) gCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc > 2:460859/1‑92 (MQ=255) cATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaa > 2:327868/1‑105 (MQ=255) cATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGaaa < 1:327868/105‑1 (MQ=255) tgtgATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACtt < 1:448294/138‑1 (MQ=255) gtgATCAACCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTg > 2:397505/1‑139 (MQ=255) aCCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca < 2:829227/56‑1 (MQ=255) aCCCCACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGca > 1:829227/1‑56 (MQ=255) ccccACACATTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCAt > 1:642298/1‑139 (MQ=255) cacacaTTATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTcc < 1:1162033/139‑1 (MQ=255) tATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc > 2:428307/1‑60 (MQ=255) tATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTc < 1:428307/60‑1 (MQ=255) tATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGc > 1:696112/1‑71 (MQ=255) tATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGc < 2:696112/71‑1 (MQ=255) tATCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCg > 1:1078785/1‑139 (MQ=255) aTCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCgg > 2:548721/1‑139 (MQ=255) aTCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCgg > 1:75475/1‑139 (MQ=255) aTCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCgg > 2:99666/1‑139 (MQ=255) aTCCGTCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCg < 1:991660/138‑1 (MQ=255) tCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTgg > 2:1135255/1‑37 (MQ=255) tCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTgg < 1:1135255/37‑1 (MQ=255) tCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt > 2:340256/1‑49 (MQ=255) tCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGt < 1:340256/49‑1 (MQ=255) tCAAATTAGCCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACtt > 1:11297/1‑139 (MQ=255) tCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAAGCACTTTGGCAAGTCACAGTTAAATAAACCTCTTTGCCTGTCATTCCACTGCCGGGACTTTATGATGaa < 2:1179439/139‑1 (MQ=255) | CCCCTTTATCTTATTTCTGATACTTAGCAGCAAATAAATAACGCGATAAAAAAAGCCAAACGTTTTCGTATTTTACAAACAACCAGAAGCTGGCATCAATTTGTGATCAACCCCACACATTATCCGTCAAATTAGTCTTTTGCAGCCGCGCGGATAATTCTGGCACACTTATTGTTAGTCCCAGGTATAGCTGTGAAAACACCAATCACTTTGGCAAGTCACAGTGAAATAAACCACTTTGCCTGTCATTCCACTACCGGGACTTTATGATGAA > NZ_CP009273/2999355‑2999628 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |