| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,015,364 | T→C | V230V (GTT→GTC) | xdhD → | molybdopterin‑dependent oxidoreductase Mo/Fe‑S‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,015,364 | 0 | T | C | 97.0% | 223.0 / ‑1.2 | 66 | V230V (GTT→GTC) | xdhD | molybdopterin‑dependent oxidoreductase Mo/Fe‑S‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (33/31); total (34/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGTTGCTGCG > NZ_CP009273/3015229‑3015496 | gCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCAc > 2:48539/1‑139 (MQ=255) aGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGt > 2:1056500/1‑139 (MQ=255) aGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGt > 1:765208/1‑139 (MQ=255) aaaCCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta < 2:116230/139‑1 (MQ=255) aaaCCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta < 2:913107/139‑1 (MQ=255) aaaCCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta < 2:741660/139‑1 (MQ=255) aCCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACt < 2:1266921/139‑1 (MQ=255) ttGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTg > 2:344678/1‑139 (MQ=255) aaGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGcc < 1:897581/138‑1 (MQ=255) gACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCgg < 2:380756/139‑1 (MQ=255) gACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCgg < 1:87343/139‑1 (MQ=255) gACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCgg < 1:385605/139‑1 (MQ=255) ccGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat < 2:186643/139‑1 (MQ=255) gcgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGatat < 1:820280/139‑1 (MQ=255) gcgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGatat < 1:678498/139‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta > 2:351851/1‑116 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta > 2:492047/1‑116 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta < 1:492047/116‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGttatta < 1:351851/116‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCt > 1:267306/1‑123 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCt < 2:267306/123‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat > 2:354987/1‑136 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat > 2:829025/1‑136 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat < 1:829025/136‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat < 1:354987/136‑1 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATc > 2:1228187/1‑139 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATc > 1:495721/1‑139 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATc > 1:468693/1‑139 (MQ=255) cgTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATc > 2:1069983/1‑139 (MQ=255) cgTAACGGCTGACGCATGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATc < 2:226164/139‑1 (MQ=255) aCGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCACCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATctac < 1:900717/138‑1 (MQ=255) cGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGggtggt > 1:765883/1‑139 (MQ=255) ttAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCagag > 1:1042081/1‑139 (MQ=255) tGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCAcc < 2:712089/139‑1 (MQ=255) cGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAAc > 1:395869/1‑139 (MQ=255) cccACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCa > 1:1274800/1‑139 (MQ=255) cacGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGcc < 1:230183/88‑1 (MQ=255) cacGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGcc > 2:230183/1‑88 (MQ=255) acGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat > 2:477556/1‑92 (MQ=255) acGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGat < 1:477556/92‑1 (MQ=255) cTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGa < 2:765883/139‑1 (MQ=255) cTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGa < 1:1069983/139‑1 (MQ=255) cTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGa < 1:812371/139‑1 (MQ=255) cTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGCGGTCAGAGCGCACCGGAACCGTCACCGCTTGa < 1:1127422/139‑1 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGatat > 2:574968/1‑89 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGatat < 1:574968/89‑1 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATctac < 2:37638/93‑1 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATctac > 1:37638/1‑93 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGAcc > 1:1006767/1‑139 (MQ=255) cACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGAcc > 1:120951/1‑139 (MQ=255) aTTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGt > 1:240687/1‑139 (MQ=255) ttACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGtt < 1:48539/139‑1 (MQ=255) tCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGc > 1:889356/1‑136 (MQ=255) tGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGa > 2:834609/1‑138 (MQ=255) gTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGaa > 2:944381/1‑53 (MQ=255) gTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGaa < 1:944381/53‑1 (MQ=255) cAGCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGt > 1:217007/1‑139 (MQ=255) gCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCagag > 2:227675/1‑85 (MQ=255) gCAAAGCTGAAGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCagag < 1:227675/85‑1 (MQ=255) aaGCCTTACCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGa < 2:889356/139‑1 (MQ=255) aCCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCa < 2:109885/66‑1 (MQ=255) aCCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCa > 1:109885/1‑66 (MQ=255) aCCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGtt > 2:205617/1‑139 (MQ=255) aCCGGGCGTCGTCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGtt > 2:1161184/1‑139 (MQ=255) cgtcgtCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGTTGCTGCg > 2:27976/1‑139 (MQ=255) cgtcgtCCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGTTGCTGCg > 2:807145/1‑139 (MQ=255) | GCAGGCGAAACCCTGCTATGTTGAAGACCGCGTAACGGCTGACGCCTGCGTCATTAAAATGTTACGTAGCCCACACGCTCACGCACTGATTACTCATCTGGATGTCAGCAAAGCTGAAGCCTTACCGGGCGTCGTTCACGTTATTACTCACCTGAATTGCCCGGATATCTACTATACCCCGGGTGGTCAGAGCGCACCGGAACCGTCACCGCTTGACCGCCGTATGTTCGGCAAGAAAATGCGTCACGTCGGCGATCGTGTTGCTGCG > NZ_CP009273/3015229‑3015496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |