Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,050,507	A→G	intergenic (‑199/+30)	ibsC ← / ← serA	type I toxin‑antitoxin system toxin IbsC/phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,050,507	0	A	G	100.0%	177.0 / NA	48	intergenic (‑199/+30)	ibsC/serA	type I toxin‑antitoxin system toxin IbsC/phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/24);  total (24/24)

CCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAA  >  NZ_CP009273/3050373‑3050639                                                                                                                                       |                                                                                                                                     ccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTg                                                                                                                                  >  2:997723/1‑139 (MQ=255)         aaTCCCCATTAAATAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGa                                                                                                                          >  2:487719/1‑139 (MQ=255)                aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGAtttt                                                                                                                      >  2:775530/1‑136 (MQ=255)                      taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                             <  2:484585/139‑1 (MQ=255)                      taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                             <  1:825981/139‑1 (MQ=255)                      taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgg                                                                                                             <  2:708340/139‑1 (MQ=255)                         acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                           >  1:193394/1‑138 (MQ=255)                           acaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                        <  2:734783/139‑1 (MQ=255)                           acaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                        >  2:416305/1‑139 (MQ=255)                               cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                                                                     >  1:337773/1‑138 (MQ=255)                               cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                                                                     >  2:741684/1‑138 (MQ=255)                                    tGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                >  2:333794/1‑138 (MQ=255)                                         tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACg                                                                                          >  2:1157301/1‑139 (MQ=255)                                               tGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGgcg                                                                                      <  1:855647/137‑1 (MQ=255)                                                gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGcgcg                                                                                    >  1:106053/1‑138 (MQ=255)                                                 atttatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGaa                                                                                  <  1:416305/139‑1 (MQ=255)                                                    tatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGTAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGCGCGCGAATg                                                                                <  2:466816/138‑1 (MQ=255)                                                    tatttTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                                                                <  1:997723/138‑1 (MQ=255)                                                      ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTa                                                                             >  1:515899/1‑139 (MQ=255)                                                                cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAg                                                                   >  2:1166101/1‑139 (MQ=255)                                                                   aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                                                                >  2:929430/1‑139 (MQ=255)                                                                   aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCtt                                                                >  1:910653/1‑139 (MQ=255)                                                                       gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                                                             <  1:1166101/138‑1 (MQ=255)                                                                             tAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                      >  1:420168/1‑139 (MQ=255)                                                                             tAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                      >  1:651480/1‑139 (MQ=255)                                                                                         cgcgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                          >  2:183693/1‑139 (MQ=255)                                                                                             gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCa                                      <  2:337773/139‑1 (MQ=255)                                                                                                      tgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcg                             <  2:910653/139‑1 (MQ=255)                                                                                                        gagaAGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgtc                           <  2:379088/139‑1 (MQ=255)                                                                                                           aaGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGc                        >  2:622188/1‑139 (MQ=255)                                                                                                            aGGGATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCt                       <  2:1173200/139‑1 (MQ=255)                                                                                                              ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                      >  2:1044297/1‑138 (MQ=255)                                                                                                              ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                      >  1:122899/1‑138 (MQ=255)                                                                                                              ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                      >  1:557086/1‑138 (MQ=255)                                                                                                              ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                      >  2:20044/1‑138 (MQ=255)                                                                                                              ggATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCtt                      >  2:135292/1‑138 (MQ=255)                                                                                                               gATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtca                    <  1:741684/139‑1 (MQ=255)                                                                                                               gATAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtca                    <  2:666893/139‑1 (MQ=255)                                                                                                                aTAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtttt                                            >  2:335144/1‑114 (MQ=255)                                                                                                                aTAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCtttt                                            <  1:335144/114‑1 (MQ=255)                                                                                                                aTAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa                   <  1:301511/139‑1 (MQ=255)                                                                                                                aTAAAAAAACGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa                   <  2:1140169/139‑1 (MQ=255)                                                                                                                       aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat            <  1:199680/139‑1 (MQ=255)                                                                                                                       aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat            <  1:775530/139‑1 (MQ=255)                                                                                                                       aaCGGGCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatatcaat            <  1:929430/139‑1 (MQ=255)                                                                                                                            gCAAGTCAGTGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACca       <  2:122899/139‑1 (MQ=255)                                                                                                                              aaGTCAGTGGCCTGCCCGTGGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACcaca     <  1:1157301/139‑1 (MQ=255)                                                                                                                                 tCAGGGGCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATaa  <  2:997655/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                     CCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAA  >  NZ_CP009273/3050373‑3050639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑