Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,085,483	A→T	I83F (ATC→TTC)	gshB →	glutathione synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,085,483	0	A	T	97.2%	267.2 / ‑0.5	71	I83F (ATC→TTC)	gshB	glutathione synthase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base T (34/35);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

TATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTT  >  NZ_CP009273/3085347‑3085616                                                                                                                                         |                                                                                                                                      tatGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTc                                                                                                                                     >  2:705096/1‑139 (MQ=255)    ggAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCt                                                                                                                                   >  1:997718/1‑138 (MQ=255)     gagaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGa                                                                                                                                 <  1:843506/139‑1 (MQ=255)      agaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGAt                                                                                                                                >  2:953474/1‑139 (MQ=255)       gaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATg                                                                                                                               >  2:654193/1‑139 (MQ=255)       gaTGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATg                                                                                                                               >  2:718901/1‑139 (MQ=255)         tGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCg                                                                                                                             >  1:110324/1‑139 (MQ=255)         tGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCg                                                                                                                             >  2:1237743/1‑139 (MQ=255)           ggCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGt                                                                                                                            >  1:270324/1‑138 (MQ=255)                    tATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCCCGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTGGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACccg                                                                                                                   <  2:199413/138‑1 (MQ=255)                    tATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGAcc                                                                                                                     <  1:705096/136‑1 (MQ=255)                    tATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACccg                                                                                                                   <  2:265606/138‑1 (MQ=255)                    tATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACccg                                                                                                                   <  1:1237743/138‑1 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACccgcc                                                                                                                 <  2:856595/134‑1 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACccgcc                                                                                                                 >  1:856595/1‑134 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGttt                                                                                                             >  2:306539/1‑138 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGttt                                                                                                             >  1:581032/1‑138 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGttt                                                                                                             >  2:578479/1‑138 (MQ=255)                          aTCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGttt                                                                                                             >  1:654601/1‑138 (MQ=255)                             aaTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACTTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGAt                                                                                                          <  2:654601/138‑1 (MQ=255)                              aTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATAc                                                                                                        >  1:888959/1‑139 (MQ=255)                              aTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATAc                                                                                                        >  1:419025/1‑139 (MQ=255)                              aTGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGGGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATAc                                                                                                        >  2:235230/1‑139 (MQ=255)                                    aaGTCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGtt                                                                                                  >  2:767466/1‑139 (MQ=255)                                    aaGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGtt                                                                                                  >  2:301060/1‑139 (MQ=255)                                         cgcgCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATc                                                                                              >  2:765839/1‑138 (MQ=255)                                           cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAc                                                                                           >  2:1265404/1‑139 (MQ=255)                                           cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAc                                                                                           >  2:1218729/1‑139 (MQ=255)                                           cgcCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAc                                                                                           >  2:312867/1‑139 (MQ=255)                                             cccATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgc                                                                                         <  2:581032/139‑1 (MQ=255)                                              ccATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTCCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTAcgcg                                                                                        <  1:654193/139‑1 (MQ=255)                                                atccccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                                                                      <  2:18730/136‑1 (MQ=255)                                                aTACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                                                                      <  1:301060/139‑1 (MQ=255)                                                aTACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCGGCCGCTGGCCGATCTCGATGTTTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                                                                      <  2:214026/139‑1 (MQ=255)                                                   cccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    <  1:629375/138‑1 (MQ=255)                                                   cccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGCCCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    <  1:270632/138‑1 (MQ=255)                                                   cccGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    <  1:540191/138‑1 (MQ=255)                                                          gCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGAcc                                                                                                                     >  1:382567/1‑98 (MQ=255)                                                          gCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGAcc                                                                                                                     <  2:382567/98‑1 (MQ=255)                                                           cTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                                                                      >  2:1167815/1‑128 (MQ=255)                                                           cTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAc                                                                                      <  1:1167815/128‑1 (MQ=255)                                                            tGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTgg                                                                           >  2:486144/1‑138 (MQ=255)                                                              aaCGTGAAGCAAAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGGATCTGCCGCGGGCCGATCTCGATGTGTTCCTGATGCGTAAAGCCCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGa                                                                          <  1:293384/137‑1 (MQ=255)                                                                 gTGAAGCAGAACTACGAAGAGTGTTTTTCGTTCGTCGGTGAACAGAATCGGCCGCTGGCCGATCTCGATGTGTCCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTg                                                                     <  2:999174/139‑1 (MQ=255)                                                                   gAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    >  2:69448/1‑122 (MQ=255)                                                                   gAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    <  1:69448/122‑1 (MQ=255)                                                                        aGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAaga                                                              <  1:767466/139‑1 (MQ=255)                                                                               cGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAgg                                                        <  2:110324/138‑1 (MQ=255)                                                                                    agTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCt                                                  >  2:899743/1‑139 (MQ=255)                                                                                     gTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTg                                                 <  1:953474/139‑1 (MQ=255)                                                                                      tGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    >  2:230050/1‑103 (MQ=255)                                                                                      tGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCt                                                                                    <  1:230050/103‑1 (MQ=255)                                                                                         ttttCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCg                                             >  2:668051/1‑139 (MQ=255)                                                                                              gttcgtCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTaa                                         >  2:282492/1‑138 (MQ=255)                                                                                                 cgtcgGTGAACGGGATCTTCCGCTTGGCAATCGGGATGTATGCCGGGAGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGAACTATATTCTGGAGCGTGCCGAAGAGAAAGGGACGCTGATCGTTAACaa                                      <  1:184083/138‑1 (MQ=255)                                                                                                   tcgGTGAACAGTATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGcc                                   >  1:404067/1‑139 (MQ=255)                                                                                                      gTGACCAGGATCTGCCGCTGGCCGATCTCGTTGTGTTCCTGAGGCTTAAAGCCCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCa                                <  2:404067/139‑1 (MQ=255)                                                                                                      gTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCa                                <  1:1218729/139‑1 (MQ=255)                                                                                                              gATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGa                                                                          >  2:375134/1‑89 (MQ=255)                                                                                                              gATCTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGa                                                                          <  1:375134/89‑1 (MQ=255)                                                                                                               aTCTGCCGCTGGCCGATCTCGATTTGTTCCGATTGCGTAAAGCCCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTgcg                       <  1:1265404/139‑1 (MQ=255)                                                                                                                 cTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCa                                >  2:433342/1‑128 (MQ=255)                                                                                                                 cTGCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCa                                <  1:433342/128‑1 (MQ=255)                                                                                                                   gCCGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGCGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTgc                        >  2:255020/1‑134 (MQ=255)                                                                                                                    ccGCTGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTgcg                       <  1:255020/134‑1 (MQ=255)                                                                                                                    ccGCGGGCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTgcg                       <  1:214769/134‑1 (MQ=255)                                                                                                                         ggCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACg                                                    >  2:1088231/1‑100 (MQ=255)                                                                                                                         ggCCGATCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACg                                                    <  1:1088231/100‑1 (MQ=255)                                                                                                                              aTCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACa                                       <  1:944929/108‑1 (MQ=255)                                                                                                                              aTCTCGATGTGTTCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACa                                       >  2:944929/1‑108 (MQ=255)                                                                                                                                    aTGTGTCCCTGAGGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGtt  <  1:847010/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                      TATGGAGATGGGCGATCTGTATCTGATCAATGGTGAAGCCCGCGCCCATACCCGCACGCTGAACGTGAAGCAGAACTACGAAGAGTGGTTTTCGTTCGTCGGTGAACAGGATCTGCCGCTGGCCGATCTCGATGTGATCCTGATGCGTAAAGACCCGCCGTTTGATACCGAGTTTATCTACGCGACCTATATTCTGGAACGTGCCGAAGAGAAAGGGACGCTGATCGTTAACAAGCCGCAGAGCCTGCGCGACTGTAACGAGAAACTGTT  >  NZ_CP009273/3085347‑3085616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑