| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,168,157 | A→T | I200N (ATC→AAC) | parE ← | DNA topoisomerase IV subunit B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,168,157 | 0 | A | T | 96.9% | 244.4 / ‑3.1 | 65 | I200N (ATC→AAC) | parE | DNA topoisomerase IV subunit B |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base T (38/25); total (39/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGT > NZ_CP009273/3168022‑3168282 | tCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTtct > 1:96260/1‑139 (MQ=255) aCCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTtctc > 1:964644/1‑138 (MQ=255) gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGc > 2:776983/1‑137 (MQ=255) gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa < 1:723778/139‑1 (MQ=255) gAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCa < 1:441527/139‑1 (MQ=255) aTTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAggg < 1:776983/139‑1 (MQ=255) cGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCt < 1:152599/139‑1 (MQ=255) ggTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCtt < 2:264742/139‑1 (MQ=255) ggTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCtt < 2:680554/139‑1 (MQ=255) cGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCttt < 2:783480/138‑1 (MQ=255) cGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGcaca < 2:964644/139‑1 (MQ=255) tCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATg > 2:298024/1‑139 (MQ=255) tCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATg > 2:853706/1‑139 (MQ=255) gACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAg < 2:96260/139‑1 (MQ=255) ttACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTg > 1:538743/1‑138 (MQ=255) ttCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGa > 1:918299/1‑138 (MQ=255) ttCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGa > 2:690969/1‑138 (MQ=255) aGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATcgcg < 2:1211952/139‑1 (MQ=255) aGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAaag > 2:1147788/1‑138 (MQ=255) aCCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAaagaag > 2:982361/1‑139 (MQ=255) tCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt > 1:704946/1‑139 (MQ=255) tCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt > 2:76361/1‑139 (MQ=255) ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAggg > 1:1103158/1‑65 (MQ=255) ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAggg < 2:1103158/65‑1 (MQ=255) ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt > 2:127850/1‑138 (MQ=255) ccTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGttt > 2:923850/1‑138 (MQ=255) aTAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCAt < 1:504164/137‑1 (MQ=255) aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 2:695372/1‑137 (MQ=255) aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 2:1039295/1‑137 (MQ=255) aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 2:665311/1‑137 (MQ=255) aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 1:515469/1‑137 (MQ=255) aCCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 1:592344/1‑137 (MQ=255) ccAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAg < 2:420497/139‑1 (MQ=255) ccAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAg < 2:704946/139‑1 (MQ=255) cAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGa < 1:232032/139‑1 (MQ=255) cGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACaa > 2:1149452/1‑56 (MQ=255) cGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACaa < 1:1149452/56‑1 (MQ=255) cGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGt > 1:1244225/1‑139 (MQ=255) ttgttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTg > 1:433432/1‑138 (MQ=255) gttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGca < 1:1100845/138‑1 (MQ=255) ttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcaca > 1:1201154/1‑139 (MQ=255) ttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcaca < 1:982361/139‑1 (MQ=255) ttCGGTATTGTTGATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGcac < 1:712165/138‑1 (MQ=255) ttgttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTAc < 2:592344/139‑1 (MQ=255) tgttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAg < 1:699840/61‑1 (MQ=255) tgttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAg > 2:699840/1‑61 (MQ=255) tgttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTAc > 1:459984/1‑138 (MQ=255) ttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCAt > 2:1280482/1‑112 (MQ=255) ttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCAt < 1:1280482/112‑1 (MQ=255) ttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCa < 1:665311/138‑1 (MQ=255) ttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAg > 2:503065/1‑139 (MQ=255) ttgATCTCATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAg > 2:916571/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 2:1187680/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 1:1041201/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 1:327524/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 2:871660/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 1:436891/1‑139 (MQ=255) ctcATCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTg > 2:453950/1‑139 (MQ=255) aTCTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGt > 1:358507/1‑139 (MQ=255) cTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc > 1:491256/1‑107 (MQ=255) cTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGc < 2:491256/107‑1 (MQ=255) cTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCacac > 1:999502/1‑120 (MQ=255) cTTTAAAAGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCacac < 2:999502/120‑1 (MQ=255) aaGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACt < 1:601750/115‑1 (MQ=255) aaGTGTTCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACt > 2:601750/1‑115 (MQ=255) | TCACCAGCGAAATTACCGATAAACGGTTTTTCCGGCAGCGTCGGCAGACCATTTACCGCTTCCGCCAGGTAATCATTCAGACCGTCCTGATAGCACCAGCGTTGTTCGGTATTGTTGATCTCATCTTTAAAAGTGATCTCAACGCCAGGGCACAATACCGCTTTGGCTTTCAGCACATGCGTCAGGCGTGAAACAGAAAATCGCGGGCTGTCAAAGAAGGTTTCATCCGGCCAGAAGTGCACACTGGTACCAGTATTGCGT > NZ_CP009273/3168022‑3168282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |