Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,266,228	C→T	G230D (GGC→GAC)	garL ←	2‑dehydro‑3‑deoxyglucarate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,266,228	0	C	T	100.0%	150.3 / NA	40	G230D (GGC→GAC)	garL	2‑dehydro‑3‑deoxyglucarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/23);  total (17/23)

TCATATCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGG  >  NZ_CP009273/3266113‑3266362                                                                                                                    |                                                                                                                                       tCATATCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCa                                                                                                                 <  2:538168/139‑1 (MQ=255)   atatCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCAt                                                                                                                <  2:655054/138‑1 (MQ=255)   atatCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATc                                                                                                               >  2:1162184/1‑139 (MQ=255)            atcTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGAc                                                                                                      <  1:563798/139‑1 (MQ=255)             tctctcTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGAcc                                                                                                     >  2:645078/1‑139 (MQ=255)              ctctctTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCg                                                                                                    >  2:1253907/1‑139 (MQ=255)                      ttgCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGGGGGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAg                                                                                             >  2:726289/1‑138 (MQ=255)                        gCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGa                                                                                                                             <  1:1092990/104‑1 (MQ=255)                        gCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGa                                                                                                                             >  2:1092990/1‑104 (MQ=255)                        gCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGa                                                                                           >  2:177864/1‑138 (MQ=255)                           gtggtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCa                                                                                                                 >  2:1150878/1‑113 (MQ=255)                           gtggtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCa                                                                                                                 <  1:1150878/113‑1 (MQ=255)                              gtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGc                                                                                     <  1:143983/138‑1 (MQ=255)                              gtggtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGc                                                                                     <  1:501920/138‑1 (MQ=255)                                 gtTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTggg                                                                                 >  1:220836/1‑139 (MQ=255)                                           aaaGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTg                                                                        <  1:527726/138‑1 (MQ=255)                                                   cAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAacgacg                                                                                                                     >  2:535498/1‑85 (MQ=255)                                                   cAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAacgacg                                                                                                                     <  1:535498/85‑1 (MQ=255)                                                    aGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCa                                                              <  1:1253907/139‑1 (MQ=255)                                                     gCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCAc                                                             <  1:887813/139‑1 (MQ=255)                                                          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGtt                                                         >  2:930228/1‑138 (MQ=255)                                                          tttCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGtt                                                         >  2:624625/1‑138 (MQ=255)                                                                aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTaaaa                                                     <  2:331850/136‑1 (MQ=255)                                                                aGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTaaaa                                                     <  1:645078/136‑1 (MQ=255)                                                                    gCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCt                                              >  2:1257074/1‑139 (MQ=255)                                                                          cGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGc                                                               <  1:1058118/116‑1 (MQ=255)                                                                          cGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGc                                                               >  2:1058118/1‑116 (MQ=255)                                                                              aGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCttt                                    <  1:261396/139‑1 (MQ=255)                                                                                  gCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGt                                <  1:187424/139‑1 (MQ=255)                                                                                  gCCGAGATCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGGGCTGAATTGCTTTTTg                                 >  2:1043117/1‑138 (MQ=255)                                                                                      agaTCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACAt                            <  1:726289/139‑1 (MQ=255)                                                                                      agaTCGCTGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACAt                            <  1:272305/139‑1 (MQ=255)                                                                                             tGCCGACAGCCACAAACGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGgtg                     >  2:629513/1‑139 (MQ=255)                                                                                                           aaCGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCga       <  1:1204695/139‑1 (MQ=255)                                                                                                           aaCGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCga       <  1:1162184/139‑1 (MQ=255)                                                                                                             cGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgaga     <  1:547805/139‑1 (MQ=255)                                                                                                             cGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgaga     <  1:1242810/139‑1 (MQ=255)                                                                                                             cGTCGCGTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCgaga     <  1:930228/139‑1 (MQ=255)                                                                                                                cgcgTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATgg  >  1:530581/1‑139 (MQ=255)                                                                                                                cgcgTCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATgg  >  1:144009/1‑139 (MQ=255)                                                                                                                    |                                                                                                                                       TCATATCAATCATCTCTCTTGTTGCGGTGGTGGTTATTTTTTAAAGGTATCAGCCAGTTTCTGAGTGGCAGAGCGGAAGACGCCGAGATCGCTGCCGACAGCCACAAACGTCGCGCCCCATTCCAGATAACGACGCGCATCGGCTTCGACCGGCGCGAGGATACCGCTGGGTTTGCCGTGCGCGCTGGCACGGTTAAAAATGTGCTGAATTGCTTTTTGTACATCCGGGTGTGATGCATTGCCGAGATGG  >  NZ_CP009273/3266113‑3266362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑