Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 249,348 G→A E51E (GAG→GAA)  yafP → GNAT family N‑acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273249,3480GA97.7% 162.2 / ‑3.7 45E51E (GAG→GAA) yafPGNAT family N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (23/20);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01

GAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAGAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTT  >  NZ_CP009273/249215‑249481
                                                                                                                                     |                                                                                                                                     
gAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACt                                                                                                                                  >  1:474879/1‑139 (MQ=255)
gAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACt                                                                                                                                  >  2:25902/1‑139 (MQ=255)
    cTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTcgc                                                                                                                               >  1:490147/1‑138 (MQ=255)
      aTCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGa                                                                                                                             >  2:29236/1‑138 (MQ=255)
       tCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGaaa                                                                                                                           >  2:1013123/1‑139 (MQ=255)
        cAGCCTGGCGCTTTTCAGCAACTATGCGCTCTTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACCACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAAt                                                                                                                          >  2:581057/1‑139 (MQ=255)
           ccTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGA‑AAACTCGCGAAATcaca                                                                                                                      <  2:384672/139‑1 (MQ=255)
                         gCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCg                                                                                                         <  1:25902/139‑1 (MQ=255)
                           aaCTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGAt                                                                                                       <  2:490147/139‑1 (MQ=255)
                                       ttttCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATg                                                                                               >  1:1233334/1‑135 (MQ=255)
                                       ttttCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATg                                                                                               <  2:1233334/135‑1 (MQ=255)
                                        tttCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCg                                                                                                         <  2:73548/124‑1 (MQ=255)
                                        tttCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCg                                                                                                         >  1:73548/1‑124 (MQ=255)
                                             ttAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     <  1:29236/139‑1 (MQ=255)
                                             ttAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     <  2:847313/139‑1 (MQ=255)
                                               agagCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggttg                                                                                   >  1:783137/1‑139 (MQ=255)
                                                 agCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggttggt                                                                                 >  2:1158246/1‑139 (MQ=255)
                                                 agCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggttggt                                                                                 >  1:387825/1‑139 (MQ=255)
                                                 agCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggttggt                                                                                 >  2:426996/1‑139 (MQ=255)
                                                 agCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCA‑AAGTGTGGGTTGCGATCATTAATGCACAACCggttggtt                                                                                >  1:49631/1‑139 (MQ=255)
                                                   cGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAgtg                                                                                                                  <  2:911932/104‑1 (MQ=255)
                                                   cGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAgtg                                                                                                                  >  1:911932/1‑104 (MQ=255)
                                                      ttACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTAt                                                                            >  1:213575/1‑139 (MQ=255)
                                                         cGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTc                                                                         <  2:783137/139‑1 (MQ=255)
                                                          gATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTcc                                                                        <  2:626879/139‑1 (MQ=255)
                                                              aCCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCAGTAATGCACAACCGGTTGGTTTTATTTCCCGCa                                                                    <  1:581057/139‑1 (MQ=255)
                                                                        aGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATta                                                          >  2:184898/1‑139 (MQ=255)
                                                                             tattCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     >  1:1087104/1‑107 (MQ=255)
                                                                             tattCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     <  2:1087104/107‑1 (MQ=255)
                                                                               ttCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGa                                                    <  1:1013123/138‑1 (MQ=255)
                                                                               ttCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGa                                                    >  2:1086644/1‑138 (MQ=255)
                                                                                  accacAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGatat                                                 <  1:1182470/138‑1 (MQ=255)
                                                                                    cacaACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCg                                                                                                         <  2:185430/80‑1 (MQ=255)
                                                                                    cacaACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCg                                                                                                         >  1:185430/1‑80 (MQ=255)
                                                                                    cacaACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     >  2:418985/1‑100 (MQ=255)
                                                                                    cacaACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCggt                                                                                     <  1:418985/100‑1 (MQ=255)
                                                                                       aacaaATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTA‑TTCCCGCGTTGAACATTATATCGATATGTTAttt                                          >  2:124628/1‑139 (MQ=255)
                                                                                               tCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGAc                                    <  1:707655/138‑1 (MQ=255)
                                                                                               tCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGAc                                    <  1:532964/138‑1 (MQ=255)
                                                                                               tCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGAc                                    <  2:1204567/138‑1 (MQ=255)
                                                                                                          gcAGATGGACGAATCTCGCGGAAAGGAAAAACGCGCGAAATCAAAAGGGTGGGTGGGGATAATTAATGCACAACCGGGGGGTTTTATTCCCCGCATGGAACATTATATCGATATGTTATTGGTTGACCGGGAATACAcc                        <  2:387825/139‑1 (MQ=255)
                                                                                                                  aCGAATCTCGCTGGAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTgg                >  2:185657/1‑139 (MQ=255)
                                                                                                                       tctcGCTGGAAGGAGAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTg           >  2:1061786/1‑139 (MQ=255)
                                                                                                                               gAAGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGTTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAgcgc    <  1:193947/138‑1 (MQ=255)
                                                                                                                                aaGGAAAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCtt  >  2:58519/1‑139 (MQ=255)
                                                                                                                                     |                                                                                                                                     
GAAACTATCAGCCTGGCGATTTTCAGCAACTATGCGCTATTTTCATTAGAGCGGTTACGATGACCGCCAGTCAGCATTATTCACCACAACAAATTTCCGCCTGGGCGCAGATTGACGAATCTCGCTGGAAGGAGAAACTCGCGAAATCACAAGTGTGGGTTGCGATCATTAATGCACAACCGGTTGGTTTTATTTCCCGCATTGAACATTATATCGATATGTTATTTGTTGACCCTGAATACACCCGCCGTGGGGTTGCCAGCGCTT  >  NZ_CP009273/249215‑249481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑