| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,300,885 | C→T | G112D (GGC→GAC) | deaD ← | ATP‑dependent RNA helicase DeaD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,300,885 | 0 | C | T | 94.9% | 209.7 / 0.7 | 59 | G112D (GGC→GAC) | deaD | ATP‑dependent RNA helicase DeaD |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (31/25); total (33/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
CATCCAGAACCAGACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGAT > NZ_CP009273/3300751‑3301020 | cATCCAGAACCAGACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGgtcg < 1:1020767/137‑1 (MQ=255)cATCCAGAACCAGACCGCTCAGTTTAGAGAGGTACAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGgtcg < 2:747815/137‑1 (MQ=255) cAGAACCAGACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTa < 1:974023/138‑1 (MQ=255) aCCAGACCGCTCAGTCTAGAGAGGACCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACagag < 1:335199/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 2:499757/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 2:479597/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 2:427520/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc > 1:499757/1‑139 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 1:409294/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 1:246847/139‑1 (MQ=255) gACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc > 1:1248594/1‑139 (MQ=255) cTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACAt > 2:740020/1‑139 (MQ=255) tttAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTAcg < 2:894790/139‑1 (MQ=255) ttAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTAcgc > 2:923375/1‑139 (MQ=255) gagagGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTAcgccg < 2:1187768/138‑1 (MQ=255) ggTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 2:921211/139‑1 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 2:131591/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 2:1193525/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 2:1030823/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 2:797382/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 1:44932/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAtgt > 1:1117516/1‑139 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 2:817444/137‑1 (MQ=255) tCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAt > 1:817444/1‑137 (MQ=255) cACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTa > 1:17743/1‑117 (MQ=255) cACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTa < 2:17743/117‑1 (MQ=255) cACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAgaga > 2:379009/1‑138 (MQ=255) ttCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTAcg < 1:489209/114‑1 (MQ=255) ttCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTAcg > 2:489209/1‑114 (MQ=255) aGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCa < 2:44932/139‑1 (MQ=255) tggtCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTg < 1:1193525/139‑1 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 2:522029/1‑139 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 2:403828/1‑139 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 1:378982/1‑139 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 2:1031499/1‑139 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 1:191952/1‑139 (MQ=255) ggCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTg > 1:389358/1‑139 (MQ=255) ggAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc > 2:663112/1‑80 (MQ=255) ggAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 2:1225523/80‑1 (MQ=255) ggAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc > 1:1225523/1‑80 (MQ=255) ggAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 1:663112/80‑1 (MQ=255) gAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGc < 2:191952/139‑1 (MQ=255) gTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCACCCTGTACCGCCa < 2:179433/139‑1 (MQ=255) cGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTc > 1:55135/1‑139 (MQ=255) aCAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTcgc > 1:990162/1‑139 (MQ=255) aCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAt < 1:1022829/86‑1 (MQ=255) aCGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCAt > 2:1022829/1‑86 (MQ=255) tCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc > 2:672499/1‑64 (MQ=255) tCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAAc < 1:672499/64‑1 (MQ=255) tCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAAt < 2:426516/96‑1 (MQ=255) tCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAAt > 1:426516/1‑96 (MQ=255) ggCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGc > 1:730707/1‑137 (MQ=255) cccTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCa > 2:82231/1‑139 (MQ=255) cgTAATTGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTgtggtg > 2:176099/1‑139 (MQ=255) gTAATTGCACGTCATAACGCTGGCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGc > 2:923838/1‑139 (MQ=255) aTTGCACGTCATAACGCTGCCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCttt < 1:625704/139‑1 (MQ=255) tGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCaa > 1:9899/1‑85 (MQ=255) tGCACGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCaa < 2:9899/85‑1 (MQ=255) aCGTCATAACGCTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCt < 2:893463/139‑1 (MQ=255) cTGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGa < 1:379009/139‑1 (MQ=255) tGGTCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGAt > 2:638330/1‑139 (MQ=255) | CATCCAGAACCAGACCGCTCAGTTTAGAGAGGTCCAGAGTGCCACGTTTCAGGTGGTCCAGCAGACGGCCCGGAGTACCGACAACGATCTGCGGCCCCTGACGCAGGGCGCGTAATTGCACGTCATAACGCTGGCCGCCGTACAGAGCAACCACATTTACGCCGCGCATGTGTTTAGAGAAATCCGTCATTGCTTCAGCAACCTGTACCGCCAGTTCGCGGGTCGGTGCCAGCACCAGAATCTGTGGTGCTTTCAGCTCAGGATCAAGAT > NZ_CP009273/3300751‑3301020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |