| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,310,402 | T→A | I162F (ATC→TTC) | nusA ← | transcription termination factor NusA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,310,402 | 0 | T | A | 95.2% | 233.8 / ‑0.1 | 63 | I162F (ATC→TTC) | nusA | transcription termination factor NusA |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (29/31); total (30/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCACT > NZ_CP009273/3310267‑3310535 | cGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACa < 2:964518/138‑1 (MQ=255) aTTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAg < 1:167494/139‑1 (MQ=255) ccGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCgtt > 2:201660/1‑139 (MQ=255) cGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCgttg < 2:586012/139‑1 (MQ=255) ggAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGcc < 2:93925/138‑1 (MQ=255) ggAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGcc < 2:637629/138‑1 (MQ=255) ggAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGcc < 2:328946/138‑1 (MQ=255) ggAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGcc < 2:1242640/138‑1 (MQ=255) gAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGcc > 2:349859/1‑137 (MQ=255) gAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATc < 1:1135251/139‑1 (MQ=255) gTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATTCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATc > 1:760501/1‑138 (MQ=255) gTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATc > 2:762619/1‑137 (MQ=255) gTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCa < 1:473356/139‑1 (MQ=255) cGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCaga > 1:1080139/1‑137 (MQ=255) gTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGAtgtt > 2:284588/1‑139 (MQ=255) cgcgCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTa > 1:187141/1‑139 (MQ=255) cgcgCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATACAGAGAGATGTTGTCGCGGTTTa > 2:378399/1‑139 (MQ=255) tCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGccc > 2:839127/1‑105 (MQ=255) tCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGccc < 1:839127/105‑1 (MQ=255) tCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACttttt > 1:934919/1‑139 (MQ=255) ggCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTcac > 1:519843/1‑139 (MQ=255) ggCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGAGCCAGAGAGATGTTGGCGCGGTTTACTTTTTTcac < 2:926862/139‑1 (MQ=255) aCGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGc > 2:201216/1‑139 (MQ=255) aCGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGc > 1:22467/1‑139 (MQ=255) aTAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGtg < 1:127860/139‑1 (MQ=255) aTAGAGCACGCCACGAACGCAGTCGCCAGGGCGGAAGTTTTCACGCGGCGGCATA‑TCTTCGCGCAGGAACCCGGATTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGGCGCGGTTTACTTTTTTCACCACGCCGGtg < 2:433791/139‑1 (MQ=255) agCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGtgatga > 2:887055/1‑139 (MQ=255) cACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGAtt > 2:995844/1‑139 (MQ=255) gCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACa > 1:869437/1‑62 (MQ=255) gCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACa < 2:869437/62‑1 (MQ=255) gCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCa > 2:600977/1‑139 (MQ=255) gCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCa > 1:481489/1‑139 (MQ=255) cACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCAcc > 1:165507/1‑139 (MQ=255) aCGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCt < 2:22467/139‑1 (MQ=255) gAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGTGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTc > 1:294220/1‑139 (MQ=255) cgGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGtt > 2:717819/1‑139 (MQ=255) ccAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGa < 2:231815/139‑1 (MQ=255) ccAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGa < 1:600977/139‑1 (MQ=255) cAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGaa < 2:187141/139‑1 (MQ=255) aGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAAc < 1:887055/139‑1 (MQ=255) ggCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGcc < 2:1151379/61‑1 (MQ=255) ggCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGcc > 1:1151379/1‑61 (MQ=255) gCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGa < 2:754656/139‑1 (MQ=255) aGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAac < 1:60564/139‑1 (MQ=255) aGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAac < 1:995844/139‑1 (MQ=255) gcagcaTA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAac < 2:977442/127‑1 (MQ=255) gcagcaTA‑TCTTCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAac > 1:977442/1‑127 (MQ=255) tCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAgagaga < 1:619226/46‑1 (MQ=255) tCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAgagaga > 2:619226/1‑46 (MQ=255) tCGCGCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCAc > 2:946579/1‑139 (MQ=255) cgcgCAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCAc < 2:481489/138‑1 (MQ=255) gcgcAGGATCACGGCTGCAGCGTTGTTGCGCAGATCCCGAGCGATGTTGTCGCAGTTTACTGTTGTCACCCCGCCGGTGATGATTTCACCTTCGTGCGCCCGGAACTGATCACCCCCCATCGCACGTTCGGCTTCACg < 2:1111003/138‑1 (MQ=255) gcgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACg < 1:1210683/138‑1 (MQ=255) gcgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGc < 1:767719/139‑1 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTcacca > 2:154870/1‑70 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTcacca < 1:154870/70‑1 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGtt < 2:283849/97‑1 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGtt > 1:283849/1‑97 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGc > 2:854051/1‑122 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGc < 1:854051/122‑1 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCa > 1:753363/1‑139 (MQ=255) cgcAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCa > 2:777635/1‑139 (MQ=255) cAGGAACACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCACt < 1:201660/139‑1 (MQ=255) | CGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATA‑TCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCACT > NZ_CP009273/3310267‑3310535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |