Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,353,444 C→A G296G (GGC→GGA)  gltD → glutamate synthase subunit GltD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,353,4440CA88.4% 136.2 / 6.1 43G296G (GGC→GGA) gltDglutamate synthase subunit GltD
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base A (18/20);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCG  >  NZ_CP009273/3353308‑3353574
                                                                                                                                        |                                                                                                                                  
aCGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGgaga                                                                                                                                  <  2:208585/139‑1 (MQ=255)
aCGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGgaga                                                                                                                                  <  1:417226/139‑1 (MQ=255)
 cGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGgaga                                                                                                                                  >  2:690981/1‑138 (MQ=255)
 cGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGGCCTTGGCGGTTGCGac                                                                                                                                 >  2:541931/1‑139 (MQ=255)
  gAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGaca                                                                                                                                <  2:901124/139‑1 (MQ=255)
  gAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGaca                                                                                                                                <  2:1062785/139‑1 (MQ=255)
  gAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGaca                                                                                                                                <  2:329140/139‑1 (MQ=255)
  gAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGaca                                                                                                                                <  2:570900/139‑1 (MQ=255)
  gAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGaca                                                                                                                                <  1:544329/139‑1 (MQ=255)
                cGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgc                                                                                                                  <  1:1267355/139‑1 (MQ=255)
                  tgtACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACtgcgt                                                                                                                <  2:601156/139‑1 (MQ=255)
                    tACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACtgcgtgc                                                                                                              >  2:68162/1‑139 (MQ=255)
                          gcgcTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGt                                                                                                        >  1:310089/1‑139 (MQ=255)
                              tGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                    >  2:609351/1‑139 (MQ=255)
                                ccGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                    <  1:1201524/137‑1 (MQ=255)
                                 cGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTgcg                                                                                                 <  1:80794/139‑1 (MQ=255)
                                      cTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAgg                                                                                            <  2:728736/139‑1 (MQ=255)
                                         aTCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAg                                                                                         <  2:815780/139‑1 (MQ=255)
                                          tCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGc                                                                                        >  2:426142/1‑139 (MQ=255)
                                                     aaaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTa                                                                             >  2:151172/1‑139 (MQ=255)
                                                      aaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTAc                                                                            >  2:1110343/1‑139 (MQ=255)
                                                        cAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCt                                                                          <  1:426142/139‑1 (MQ=255)
                                                        cAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCt                                                                          <  2:619019/139‑1 (MQ=255)
                                                           ttAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTcc                                                                                                      >  1:865523/1‑108 (MQ=255)
                                                           ttAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTcc                                                                                                      <  2:865523/108‑1 (MQ=255)
                                                           ttAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCtgtg                                                                       >  1:775626/1‑139 (MQ=255)
                                                            tAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGc                                                                      >  2:107811/1‑139 (MQ=255)
                                                                   cTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                    >  1:594163/1‑102 (MQ=255)
                                                                   cTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGt                                                                                                    <  2:594163/102‑1 (MQ=255)
                                                                     ttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGa                                                                                                                          <  2:752336/78‑1 (MQ=255)
                                                                     ttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGa                                                                                                                          >  1:752336/1‑78 (MQ=255)
                                                                     ttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTAcc                                                                           >  2:1231597/1‑125 (MQ=255)
                                                                     ttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTAcc                                                                           <  1:1231597/125‑1 (MQ=255)
                                                                     ttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGAC‑‑‑‑TGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGtgat                                                         >  2:170990/1‑139 (MQ=255)
                                                                      tGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTAtcgtc                                                              <  1:1110343/137‑1 (MQ=255)
                                                                                cgcgACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTcc                                                                                                      >  2:119282/1‑87 (MQ=255)
                                                                                cgcgACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTcc                                                                                                      <  1:119282/87‑1 (MQ=255)
                                                                                cgcgACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAagag                                                   >  1:54897/1‑138 (MQ=255)
                                                                                 gcgACGAACCGTCCGCCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGaa                                                 <  1:541931/139‑1 (MQ=255)
                                                                                            ttCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGtt                                      >  1:788195/1‑139 (MQ=255)
                                                                                                               aaCGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGaaaaa                   >  1:1078695/1‑139 (MQ=255)
                                                                                                               aaCGCGTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGaaaaa                   <  1:15703/139‑1 (MQ=255)
                                                                                                                   cgTGGTGGTCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTATGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAAcgcg               >  1:857194/1‑139 (MQ=255)
                                                                                                                          gtCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaag        <  1:609351/139‑1 (MQ=255)
                                                                                                                          gtCCTTGGCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGgaag        <  2:973257/139‑1 (MQ=255)
                                                                                                                                ggCGGTGGAGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCg  >  1:102116/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                  
ACGAAGACGCCGATGGCGTGTACGCAGCGCTGCCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTACGTCCGTGCGCCAGGGAGCGAAGCACGTTACCTGTGCCTATCGTCGTGATGAAGAGAACATGCCGGGTTCCCGCCGCGAAGTGAAAAACGCGCGGGAAGAAGGCG  >  NZ_CP009273/3353308‑3353574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑