| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,394,698 | T→C | intergenic (‑252/+53) | mreB ← / ← csrD | rod shape‑determining protein MreB/RNase E specificity factor CsrD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,394,698 | 0 | T | C | 98.4% | 219.6 / NA | 64 | intergenic (‑252/+53) | mreB/csrD | rod shape‑determining protein MreB/RNase E specificity factor CsrD |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (33/30); total (34/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TTGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCC > NZ_CP009273/3394570‑3394828 | ttGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAGAc > 2:586274/1‑139 (MQ=255)ttGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGAc > 2:1130747/1‑139 (MQ=255) aCAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAgc > 1:1132362/1‑139 (MQ=255) aCAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCAGCAACAATCTGGCGAGCAGACAAGCACACCCGCATGAGACGCAgc > 2:492843/1‑139 (MQ=255) cAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAgc < 1:1290880/138‑1 (MQ=255) cAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAgc < 1:466019/138‑1 (MQ=255) ctctGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCACCCGCGTACGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCat > 1:572008/1‑139 (MQ=255) tGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCattat > 2:419319/1‑139 (MQ=255) tGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCa < 2:229568/135‑1 (MQ=255) gTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGt > 1:1038387/1‑139 (MQ=255) gTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGt > 2:735329/1‑139 (MQ=255) ggCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGa > 2:984128/1‑139 (MQ=255) gCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGAt > 2:108500/1‑139 (MQ=255) cTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGAt > 2:438761/1‑138 (MQ=255) cTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGAt > 2:424163/1‑138 (MQ=255) cTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGAt > 2:1126541/1‑138 (MQ=255) cTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGAt > 1:841709/1‑138 (MQ=255) cATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGc < 1:108500/139‑1 (MQ=255) tCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAg > 2:20587/1‑139 (MQ=255) ttCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTa < 1:1153578/134‑1 (MQ=255) ttCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTa > 2:1153578/1‑134 (MQ=255) cGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACGCCCCCATGAGACGCAgc < 2:98989/89‑1 (MQ=255) cGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCACCATGAGACGCAgc > 1:98989/1‑89 (MQ=255) gCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATc > 2:443379/1‑139 (MQ=255) gTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTtg > 1:98145/1‑139 (MQ=255) tttGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCttt < 2:1038387/137‑1 (MQ=255) tttGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTtgt > 1:1263363/1‑139 (MQ=255) cgTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGc > 2:378418/1‑107 (MQ=255) cgTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGc < 1:378418/107‑1 (MQ=255) cgTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAttttt > 2:1075781/1‑139 (MQ=255) gTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt < 2:332588/139‑1 (MQ=255) gTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt < 1:443379/139‑1 (MQ=255) gTAAGGTCATTCATCTGCATATGTGCTGCAACAAGCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt < 1:492843/139‑1 (MQ=255) ggTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACg < 2:1263363/139‑1 (MQ=255) ggTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACg < 1:984128/139‑1 (MQ=255) ggTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACg < 2:98145/139‑1 (MQ=255) gTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGt < 2:1079166/139‑1 (MQ=255) gTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGt < 2:1132362/139‑1 (MQ=255) ttcatCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAg > 2:308880/1‑138 (MQ=255) ttcatCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAg > 2:630989/1‑138 (MQ=255) ttcatCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAg > 2:569768/1‑138 (MQ=255) cATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCaa < 2:907588/136‑1 (MQ=255) cATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAg < 1:419319/137‑1 (MQ=255) cATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAg < 2:1086845/137‑1 (MQ=255) cATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAg < 2:446156/137‑1 (MQ=255) gctgcAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCtgt > 1:728874/1‑137 (MQ=255) ctgcAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAg > 1:1180450/1‑139 (MQ=255) ctgcAACAATCCGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt < 2:677577/114‑1 (MQ=255) ctgcAACAATCCGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATAtttttt > 1:677577/1‑114 (MQ=255) gcAACAATCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGa > 2:1171041/1‑139 (MQ=255) acaaTCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGAcg < 1:1032254/138‑1 (MQ=255) acaaTCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGAcg > 2:1032254/1‑138 (MQ=255) tCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAAc < 1:1062954/60‑1 (MQ=255) tCTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAAc > 2:1062954/1‑60 (MQ=255) cTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACg > 1:630761/1‑69 (MQ=255) cTGGCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACg < 2:630761/69‑1 (MQ=255) gCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACg > 1:806704/1‑105 (MQ=255) gCGAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACg < 2:806704/105‑1 (MQ=255) gAGCAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATcc < 1:308880/139‑1 (MQ=255) cAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTaaa < 1:668114/71‑1 (MQ=255) cAGACAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTaaa > 2:668114/1‑71 (MQ=255) cAAGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTT‑AACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc < 1:1130747/139‑1 (MQ=255) aaGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAACCGGCAGTTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGGGAGGACGCGAAAAAATCCCCTTGccc < 2:1246880/139‑1 (MQ=255) aaGCACACCCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGccc < 1:1126541/139‑1 (MQ=255) tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGc > 1:830091/1‑129 (MQ=255) tCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGc < 2:830091/129‑1 (MQ=255) | TTGTTACAAATCTCTGCCTGAGTCCAGAAAGGCTTAATGCATCAGCAGCATTCCTCGCCTGTTTGCAACCGCGTAAGGTCATTCATCTGCATATGTGCTGCAACAATCTGGCGAGCAGACAAGCACACTCCCATGAGACGCAGCGCGCATTATTCTACGTGAAAACGGATTAAACGGCAGGTTAAACCGAGTATCTTTGTGAATATTTTTTCACGTTAGTATCAAGTGGCTGTGAGGACGCGAAAAAATCCCCTTGCCC > NZ_CP009273/3394570‑3394828 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |