| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,403,270 | A→G | E281G (GAA→GGA) | prmA → | 50S ribosomal protein L11 methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,403,270 | 0 | A | G | 97.9% | 163.8 / ‑3.8 | 47 | E281G (GAA→GGA) | prmA | 50S ribosomal protein L11 methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (23/23); total (23/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
TTACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGAAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAATTTGCGTGCC > NZ_CP009273/3403134‑3403404 | ttACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGgaa < 1:801576/139‑1 (MQ=255) cGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAaag < 2:904863/139‑1 (MQ=255) aaCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGgaa < 1:110403/132‑1 (MQ=255) aaCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGgaa > 2:110403/1‑132 (MQ=255) aaCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAaagaa > 1:18086/1‑137 (MQ=255) aCTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAagag < 1:1098135/139‑1 (MQ=255) gCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggt < 1:233164/139‑1 (MQ=255) gCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtgg < 2:961190/138‑1 (MQ=255) gCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtgg < 1:909980/138‑1 (MQ=255) cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg < 1:1287982/139‑1 (MQ=255) cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg < 2:590901/139‑1 (MQ=255) cACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggtg < 1:237192/139‑1 (MQ=255) ttAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTa > 2:595254/1‑139 (MQ=255) aaTCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTAt < 1:825079/138‑1 (MQ=255) aGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGAAAAAGAAGAGTGGTGCCGTATTAcc < 1:265942/138‑1 (MQ=255) cGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGt > 1:782952/1‑139 (MQ=255) tCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGc > 1:899411/1‑125 (MQ=255) tCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGc < 2:899411/125‑1 (MQ=255) tCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCg > 2:746987/1‑139 (MQ=255) ccTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGt < 2:151793/139‑1 (MQ=255) cTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGt < 2:1251700/138‑1 (MQ=255) aGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCt > 2:400779/1‑139 (MQ=255) gTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa < 2:859343/139‑1 (MQ=255) gTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa < 1:971670/139‑1 (MQ=255) gTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa < 1:594830/139‑1 (MQ=255) gTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa < 1:578490/139‑1 (MQ=255) gCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCg < 1:400779/138‑1 (MQ=255) ttCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggt < 1:100261/92‑1 (MQ=255) ttCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAgtggt > 2:100261/1‑92 (MQ=255) ccGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGAc > 1:827680/1‑139 (MQ=255) gCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAg > 1:760997/1‑139 (MQ=255) aGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGc > 1:1028747/1‑139 (MQ=255) ccAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa < 2:690633/108‑1 (MQ=255) ccAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCa > 1:690633/1‑108 (MQ=255) aGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGc < 1:381375/139‑1 (MQ=255) aGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGc < 1:595254/139‑1 (MQ=255) gagCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTca > 1:1290103/1‑139 (MQ=255) tttGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACaa > 1:72664/1‑137 (MQ=255) aaGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACg > 2:25940/1‑139 (MQ=255) ttATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAg > 1:1016890/1‑120 (MQ=255) ttATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAg < 2:1016890/120‑1 (MQ=255) tATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCAt < 2:807037/84‑1 (MQ=255) tATGCCGATAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCAt > 1:807037/1‑84 (MQ=255) tAGCTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGat > 1:325191/1‑139 (MQ=255) cTTCGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGattat > 1:424225/1‑139 (MQ=255) cGCACTGGACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTa > 2:848065/1‑139 (MQ=255) gACCCGGTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAAt > 1:1265593/1‑139 (MQ=255) ggTCGTGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAATTTGCg > 2:279219/1‑139 (MQ=255) tGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAATTTGCGTGcc > 2:1056230/1‑138 (MQ=255) tGGGAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAATTTGCGTGcc > 1:896712/1‑138 (MQ=255) | TTACGTGAACTGGCACCGTTAATCAGCGTCCTGCCGGTTTCAGGCGGTTTGCTGGGCCTTTCCGGTATTCTGGCAAGCCAGGCAGAGAGCGTTTGTGAAGCTTATGCCGATAGCTTCGCACTGGACCCGGTCGTGGAAAAAGAAGAGTGGTGCCGTATTACCGGTCGTAAGAATTAACCTTCGCATCGCCGTAGGGTGACGCGGGGCGAAGTGCGAGCAAGCTCACAAAAGGCACGTAAATTTGCCGATTATTTACGCAAATTTGCGTGCC > NZ_CP009273/3403134‑3403404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |