Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,413,569	T→C	I26T (ATC→ACC)	yhdX →	amino acid ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,413,569	0	T	C	97.3%	243.9 / ‑1.6	73	I26T (ATC→ACC)	yhdX	amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (32/39);  total (33/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.04e-02

CGAGCGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGATCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTATTGTC  >  NZ_CP009273/3413441‑3413696                                                                                                                                 |                                                                                                                                cGAGCGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCgtt                                                                                                                       >  1:467207/1‑139 (MQ=255) cGAGCGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCgtt                                                                                                                       >  2:1118478/1‑139 (MQ=255)     cGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGTCCCTTGCCGttgttg                                                                                                                   >  1:999056/1‑139 (MQ=255)     cGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGttgttg                                                                                                                   >  1:1134653/1‑139 (MQ=255)       tCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCt                                                                                                                              <  1:769245/126‑1 (MQ=255)       tCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCt                                                                                                                              >  2:769245/1‑126 (MQ=255)       tCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCt                                                                                                                 >  2:364988/1‑139 (MQ=255)              ggcggcGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCg                                                                                                          >  2:1134450/1‑139 (MQ=255)                  gcgcTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCAt                                                                                                      >  2:293409/1‑139 (MQ=255)                            cAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTgttggtt                                                                                              <  1:721238/137‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:350236/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:733770/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:556571/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  2:514113/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:364988/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:113570/139‑1 (MQ=255)                             aGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  1:1134450/139‑1 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCTTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  1:1124414/139‑1 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           >  1:392581/1‑137 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTtggttggt                                                                                           <  2:392581/137‑1 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  2:201461/139‑1 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         >  2:1124414/1‑139 (MQ=255)                               cGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  1:1118478/139‑1 (MQ=255)                                    tGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTca                                                                                    <  1:263023/139‑1 (MQ=255)                                    tGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTca                                                                                    <  1:442426/139‑1 (MQ=255)                                    tGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTca                                                                                    >  2:442426/1‑139 (MQ=255)                                         tCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         >  2:758163/1‑129 (MQ=255)                                         tCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  1:758163/129‑1 (MQ=255)                                         tCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCacaaca                                                                               >  1:788795/1‑139 (MQ=255)                                                tttCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAAc                                                                        >  2:973871/1‑139 (MQ=255)                                                  tCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTaa                                                                         <  1:682394/136‑1 (MQ=255)                                                  tCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTaa                                                                         >  2:682394/1‑136 (MQ=255)                                                  tCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACg                                                                       >  2:312708/1‑138 (MQ=255)                                                          tcATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCaa                                                               >  1:605378/1‑138 (MQ=255)                                                          tcATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCaa                                                               >  1:466939/1‑138 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGATCCTTGCCGTTGTTGCTGTTGTCGGCATTgttgg                                                                                                <  1:747935/103‑1 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGATCCTTGCCGTTGTTGCTGTTGTCGGCATTgttgg                                                                                                >  2:747935/1‑103 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTca                                                                                    <  2:1207776/115‑1 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTca                                                                                    >  1:1207776/1‑115 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCaataa                                                            >  1:44007/1‑139 (MQ=255)                                                            aTCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCaa                                                               <  1:288382/136‑1 (MQ=255)                                                               gccgcTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCg                                                         <  2:1134653/139‑1 (MQ=255)                                                                 cgcTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTcac                                                                                   >  1:773119/1‑111 (MQ=255)                                                                 cgcTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTcac                                                                                   <  2:773119/111‑1 (MQ=255)                                                                   cTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         >  1:1097934/1‑103 (MQ=255)                                                                   cTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  2:1097934/103‑1 (MQ=255)                                                                   cTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTgg                                                      >  1:1252562/1‑138 (MQ=255)                                                                   cTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGc                                                     <  1:126168/139‑1 (MQ=255)                                                                      aaCCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCa                                                    <  2:283409/137‑1 (MQ=255)                                                                        ccGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTAc                                                >  1:1230260/1‑139 (MQ=255)                                                                          gTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACtt                                              <  2:788795/139‑1 (MQ=255)                                                                                     aCTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCt                                                                                                                              >  2:828873/1‑48 (MQ=255)                                                                                     aCTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCt                                                                                                                              <  1:828873/48‑1 (MQ=255)                                                                                     aCTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTg                                     <  2:1230260/137‑1 (MQ=255)                                                                                        ctctTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCtgtt                                                                                                              <  1:1006336/61‑1 (MQ=255)                                                                                        ctctTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCtgtt                                                                                                              >  2:1006336/1‑61 (MQ=255)                                                                                           ttttGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCttt                                <  1:312708/136‑1 (MQ=255)                                                                                                   aCCCTACGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCgcgg                     >  1:430723/1‑139 (MQ=255)                                                                                                         cGGTTCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCt                 <  2:605378/137‑1 (MQ=255)                                                                                                            ttCGCGCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCtt            <  2:1145550/139‑1 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         >  2:682581/1‑60 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTa                                                                                         <  1:682581/60‑1 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCt                 <  1:785994/132‑1 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCt                 >  2:785994/1‑132 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCg          >  1:813336/1‑139 (MQ=255)                                                                                                              cgcgCCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCg          >  2:329503/1‑139 (MQ=255)                                                                                                               gcgcCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCgg         >  2:627009/1‑139 (MQ=255)                                                                                                               gcgcCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCg          <  2:345856/138‑1 (MQ=255)                                                                                                                 gcCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTa       <  2:559994/139‑1 (MQ=255)                                                                                                                 gcCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTa       <  2:467207/139‑1 (MQ=255)                                                                                                                 gcCTGGTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTa       <  1:293409/139‑1 (MQ=255)                                                                                                                      gTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTATTGTc  <  1:973871/139‑1 (MQ=255)                                                                                                                      gTTATTCCAGACCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTATTGTc  <  2:430723/139‑1 (MQ=255)                                                                                                                                 |                                                                                                                                CGAGCGTCGCTACGGCGGCGCTCTCTTCAGCGTTATGCTTTCCGAGGTTTCTATGTCTCATCGCCGCTCAACCGTTAAAGGCTCACTCTCTTTTGCCAACCCTACGGTTCGCGCCTGGTTATTCCAGATCCTTGCCGTTGTTGCTGTTGTCGGCATTGTTGGTTGGTTATTTCACAACACTGTAACGAATCTCAATAATCGTGGCATTACTTCAGGTTTTGCCTTTCTGGATCGCGGCGCTGGCTTCGGTATTGTC  >  NZ_CP009273/3413441‑3413696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑