Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,429,447 T→G G294G (GGT→GGG)  rsmB → 16S rRNA (cytosine(967)‑C(5))‑methyltransferase RsmB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,429,4470TG100.0% 248.6 / NA 63G294G (GGT→GGG) rsmB16S rRNA (cytosine(967)‑C(5))‑methyltransferase RsmB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/32);  total (31/32)

TTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATG  >  NZ_CP009273/3429323‑3429581
                                                                                                                            |                                                                                                                                      
tttGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGt                                                                                                                          >  1:1254018/1‑139 (MQ=255)
  tgtgCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGAcc                                                                                                                            <  2:310786/135‑1 (MQ=255)
         cccccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAgg                                                                                                                 >  2:100949/1‑139 (MQ=255)
            ccGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGa                                                                                                              <  2:708236/139‑1 (MQ=255)
             cggcggTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGAt                                                                                                             <  1:1035941/139‑1 (MQ=255)
              ggcggTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACATGGTGATg                                                                                                            >  1:548807/1‑139 (MQ=255)
                     aaaCAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                     <  1:21571/139‑1 (MQ=255)
                     aaaCAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTa                                                                                                     <  1:1006353/139‑1 (MQ=255)
                       acaacGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                                   <  1:218586/139‑1 (MQ=255)
                       acaacGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                                   <  1:537303/139‑1 (MQ=255)
                              atatCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCa                                                                                            <  1:81203/139‑1 (MQ=255)
                                 tCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATg                                                                                         <  1:1185142/139‑1 (MQ=255)
                                     tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtgt                                                                                     >  1:866991/1‑139 (MQ=255)
                                     tGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtgt                                                                                     >  1:750196/1‑139 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGtgtg                                                                                    >  1:1170960/1‑133 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGtgtg                                                                                    <  2:1170960/133‑1 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                               >  1:262317/1‑138 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                               >  2:355600/1‑138 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                               >  2:1173763/1‑138 (MQ=255)
                                            gCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAg                                                                               >  1:459872/1‑138 (MQ=255)
                                             cACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGc                                                                              >  1:449572/1‑138 (MQ=255)
                                                      cgcAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGc                                                                              >  1:1247304/1‑129 (MQ=255)
                                                      cgcAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGc                                                                              <  2:1247304/129‑1 (MQ=255)
                                                      cgcAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGa                                                                    >  1:150129/1‑139 (MQ=255)
                                                       gcAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGAt                                                                   <  2:750196/139‑1 (MQ=255)
                                                        cAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGAt                                                                                                             >  1:201049/1‑96 (MQ=255)
                                                        cAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGAt                                                                                                             <  2:201049/96‑1 (MQ=255)
                                                        cAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATc                                                                  >  1:493425/1‑139 (MQ=255)
                                                        cAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATc                                                                  >  1:186859/1‑139 (MQ=255)
                                                           gttgttGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCa                                                               <  1:100949/139‑1 (MQ=255)
                                                           gttgttGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCa                                                               >  1:355469/1‑139 (MQ=255)
                                                                tgCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTtta                                                          >  2:449296/1‑139 (MQ=255)
                                                                           tCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGcc                                               <  1:1019412/139‑1 (MQ=255)
                                                                              acgaACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtg                                                                                      >  1:867918/1‑97 (MQ=255)
                                                                              acgaACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGgtg                                                                                      <  2:867918/97‑1 (MQ=255)
                                                                                       gcCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAAc                                   <  1:1056283/139‑1 (MQ=255)
                                                                                       gcCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGAGCGCATTTTATTAGATGCGCCTTGTTCAGCAAc                                   <  1:1112309/139‑1 (MQ=255)
                                                                                                gggTTTACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGAt                          <  2:75234/139‑1 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACTGTGAAAc                                                                                                                     >  2:850404/1‑43 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAAc                                                                                                                     <  1:850404/43‑1 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGAt                                                                                                             <  2:264123/51‑1 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGAt                                                                                                             >  1:264123/1‑51 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCt                                                                                                 <  1:635617/63‑1 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCt                                                                                                 >  2:635617/1‑63 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCaa                                    >  2:1088444/1‑124 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCaa                                    <  1:1088444/124‑1 (MQ=255)
                                                                                                     tACGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATtcgt                      <  2:1214224/138‑1 (MQ=255)
                                                                                                      aCGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                                   <  1:580359/60‑1 (MQ=255)
                                                                                                      aCGACAATTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTAcc                                                                                                   >  2:580359/1‑60 (MQ=255)
                                                                                                           aaTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc               <  1:523975/139‑1 (MQ=255)
                                                                                                            aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGAt                                                    >  1:902961/1‑101 (MQ=255)
                                                                                                            aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGAt                                                    <  2:902961/101‑1 (MQ=255)
                                                                                                            aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAAc                                   <  2:248459/118‑1 (MQ=255)
                                                                                                            aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAAc                                   >  1:248459/1‑118 (MQ=255)
                                                                                                            aTTTAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc               >  2:665654/1‑138 (MQ=255)
                                                                                                             tttAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATc               >  1:801104/1‑137 (MQ=255)
                                                                                                             tttAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCa             <  1:603222/139‑1 (MQ=255)
                                                                                                               tAAAACGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAg            <  1:56240/138‑1 (MQ=255)
                                                                                                                    cGCCTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTa      <  1:1173763/139‑1 (MQ=255)
                                                                                                                       cTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGc                   >  2:20409/1‑123 (MQ=255)
                                                                                                                       cTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGc                   <  1:20409/123‑1 (MQ=255)
                                                                                                                       cTTGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAAt   >  1:159523/1‑139 (MQ=255)
                                                                                                                        ttGGGATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATg  >  1:413033/1‑139 (MQ=255)
                                                                                                                            |                                                                                                                                      
TTTGTGCCGCCCCCGGCGGTAAAACAACGCATATCCTTGAGGTGGCACCAGAAGCGCAGGTTGTTGCGGTTGATATCGACGAACAGCGCCTCTCTCGGGTTTACGACAATTTAAAACGCCTTGGTATGAAGGCGACCGTGAAACAAGGTGATGGCCGTTACCCTTCTCAATGGTGTGGCGAGCAACAGTTTGATCGCATTTTATTAGATGCGCCTTGTTCAGCAACCGGTGTGATTCGTCGCCATCCAGATATTAAATG  >  NZ_CP009273/3429323‑3429581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑