| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,539,325 | C→T | L115L (CTG→TTG) | nfuA → | Fe‑S biogenesis protein NfuA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,539,325 | 0 | C | T | 90.6% | 163.0 / 8.2 | 53 | L115L (CTG→TTG) | nfuA | Fe‑S biogenesis protein NfuA |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (26/22); total (28/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
ACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGC > NZ_CP009273/3539192‑3539457 | aCCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAg > 2:19909/1‑139 (MQ=255)aCCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAg > 2:41257/1‑139 (MQ=255) ccATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGt > 2:474302/1‑139 (MQ=255) ccATACCTGGAAGAGGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGt < 2:817066/139‑1 (MQ=255) cATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTc > 2:849608/1‑139 (MQ=255) gAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGAt < 1:760000/137‑1 (MQ=255) gAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATc > 2:826078/1‑138 (MQ=255) gAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATc > 1:257824/1‑138 (MQ=255) gAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGAt < 1:568626/137‑1 (MQ=255) aGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAAc > 2:1077949/1‑139 (MQ=255) gATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAAcc < 2:340323/139‑1 (MQ=255) tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGGGTATATGTTGCAGTCGCAGATCAACCca > 1:574206/1‑139 (MQ=255) tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca > 2:266641/1‑139 (MQ=255) tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca < 2:353108/139‑1 (MQ=255) tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca < 1:33781/139‑1 (MQ=255) tGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca > 2:810576/1‑139 (MQ=255) gaTCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCtt < 1:232943/139‑1 (MQ=255) gaTCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCtt < 1:1006840/139‑1 (MQ=255) aTCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTg < 1:826078/139‑1 (MQ=255) gATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTg > 1:407385/1‑139 (MQ=255) ggTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCg < 1:41257/138‑1 (MQ=255) cccAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGAt > 1:486528/1‑139 (MQ=255) ccAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGAt < 1:266641/138‑1 (MQ=255) aGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTg > 1:206727/1‑111 (MQ=255) aGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTg < 2:206727/111‑1 (MQ=255) aaaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCg > 1:657181/1‑136 (MQ=255) aaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGa > 2:775042/1‑139 (MQ=255) aaGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGCGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGa > 2:747863/1‑139 (MQ=255) cccGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCa < 1:203316/99‑1 (MQ=255) cccGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCa > 2:203316/1‑99 (MQ=255) gCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCa < 2:1142215/139‑1 (MQ=255) aaTGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTg < 2:602830/139‑1 (MQ=255) gTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca > 1:206100/1‑68 (MQ=255) gTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCca < 2:206100/68‑1 (MQ=255) aGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggc > 2:285380/1‑139 (MQ=255) gCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTggcggc < 1:422101/138‑1 (MQ=255) aTGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTaa < 1:474302/139‑1 (MQ=255) tGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAc > 2:521796/1‑139 (MQ=255) tGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAc > 1:539402/1‑139 (MQ=255) tGCACCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAAc > 1:589833/1‑139 (MQ=255) aCCGCTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgt < 1:1212146/139‑1 (MQ=255) cTGATGGAGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATACTGCAATTTGGCGGCGGCTGTAACGgttgtt > 1:1039403/1‑139 (MQ=255) aGCGCGTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGt < 1:19909/139‑1 (MQ=255) gcgcGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTc > 2:1005572/1‑139 (MQ=255) cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgt > 2:23357/1‑124 (MQ=255) cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGgt < 1:23357/124‑1 (MQ=255) cgTGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGGTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAt > 1:305445/1‑139 (MQ=255) tGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCATCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtgt > 2:492978/1‑139 (MQ=255) tGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTc > 2:1101777/1‑96 (MQ=255) tGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTc < 1:1101777/96‑1 (MQ=255) tGGAGTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGAtgt < 2:1269802/139‑1 (MQ=255) gTATATGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACg < 1:47926/139‑1 (MQ=255) tataTGTTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGc < 2:1191352/139‑1 (MQ=255) | ACCATACCTGGAAGATGCAGAGATCGATTTTGTTACCGACCAGTTGGGTTCCCAGTTAACGCTGAAAGCCCCGAACGCCAAAATGCGTAAAGTGGCAGACGATGCACCGCTGATGGAGCGCGTGGAGTATATGCTGCAGTCGCAGATCAACCCACAGCTTGCTGGTCACGGTGGTCGCGTTTCGCTGATGGAAATCACCGAAGACGGTTACGCCATTCTGCAATTTGGCGGCGGCTGTAACGGTTGTTCCATGGTCGATGTGACGC > NZ_CP009273/3539192‑3539457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |