Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,543,436 T→C intergenic (‑7/+3) malQ ← / ← malP 4‑alpha‑glucanotransferase/maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,543,4360TC96.3% 295.0 / 1.1 82intergenic (‑7/+3)malQ/malP4‑alpha‑glucanotransferase/maltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (36/43);  total (38/44)
Fisher's exact test for biased strand distribution p-value = 5.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

GATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACC  >  NZ_CP009273/3543303‑3543571
                                                                                                                                     |                                                                                                                                       
gATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCtt                                                                                                                                     <  1:131030/138‑1 (MQ=255)
 aTGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAg                                                                                                                                   >  1:562787/1‑139 (MQ=255)
 aTGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAg                                                                                                                                   >  2:697817/1‑139 (MQ=255)
    cATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGt                                                                                                                                >  2:1203899/1‑139 (MQ=255)
      tCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGttt                                                                                                                              >  1:1021636/1‑139 (MQ=255)
      tCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGttt                                                                                                                              >  1:284848/1‑139 (MQ=255)
        gcgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtg                                                                                                                            <  1:800888/139‑1 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           >  2:662933/1‑139 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           >  1:826171/1‑139 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           <  1:1267419/139‑1 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           >  1:508381/1‑139 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           >  1:486230/1‑139 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           >  2:974714/1‑139 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgc                                                                                                                           <  2:409713/139‑1 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtg                                                                                                                            <  2:570428/138‑1 (MQ=255)
         cgTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtg                                                                                                                            >  1:570428/1‑138 (MQ=255)
          gTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgcc                                                                                                                          <  2:200293/139‑1 (MQ=255)
             aaGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgcctg                                                                                                                        <  2:518083/138‑1 (MQ=255)
                  aaCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGAt                                                                                                                  <  1:905416/139‑1 (MQ=255)
                   aCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATa                                                                                                                 <  1:11466/139‑1 (MQ=255)
                   aCGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATa                                                                                                                 >  2:128805/1‑139 (MQ=255)
                                 cGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATc                                                                                                   >  1:313528/1‑139 (MQ=255)
                                     gcTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGa                                                                                               <  1:328086/139‑1 (MQ=255)
                                          tCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATaga                                                                                          <  1:697817/139‑1 (MQ=255)
                                                 cGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     <  1:1240652/137‑1 (MQ=255)
                                                 cGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATcc                                                                                   >  1:1117332/1‑139 (MQ=255)
                                                 cGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCAACGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     <  2:169845/137‑1 (MQ=255)
                                                           tGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACa                                                                         >  1:654444/1‑139 (MQ=255)
                                                             ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     >  1:834856/1‑125 (MQ=255)
                                                             ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     <  2:834856/125‑1 (MQ=255)
                                                             ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATa                                                                       >  1:820022/1‑139 (MQ=255)
                                                             ggCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATa                                                                       >  2:355975/1‑139 (MQ=255)
                                                                  tGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCg                                                                    >  1:378176/1‑137 (MQ=255)
                                                                    aTGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATGCCGCAgc                                                                <  1:164687/139‑1 (MQ=255)
                                                                    aTGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgc                                                                <  1:451195/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:284848/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:1021636/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:1030974/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  1:1203899/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  1:385094/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  1:781394/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  1:682927/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:820022/139‑1 (MQ=255)
                                                                       tAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:562787/139‑1 (MQ=255)
                                                                         aTTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcgggcg                                                           <  2:378176/139‑1 (MQ=255)
                                                                              ggCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgcctg                                                                                                                        >  1:1055727/1‑73 (MQ=255)
                                                                              ggCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtgcctg                                                                                                                        <  2:1055727/73‑1 (MQ=255)
                                                                              ggCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTAtt                                                      >  1:565837/1‑139 (MQ=255)
                                                                              ggCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTAtt                                                      >  1:238948/1‑139 (MQ=255)
                                                                               gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAg                                                                                <  1:545122/112‑1 (MQ=255)
                                                                               gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAg                                                                                >  2:545122/1‑112 (MQ=255)
                                                                               gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             >  1:103785/1‑131 (MQ=255)
                                                                               gCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAgcggg                                                             <  2:103785/131‑1 (MQ=255)
                                                                                        cgccgcCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtg                                                                                                                            <  1:43663/59‑1 (MQ=255)
                                                                                        cgccgcCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTtg                                                                                                                            >  2:43663/1‑59 (MQ=255)
                                                                                        cgccgcCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACg                                                                                                               <  2:99556/72‑1 (MQ=255)
                                                                                        cgccgcCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACg                                                                                                               >  1:99556/1‑72 (MQ=255)
                                                                                         gccgccAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     >  1:434939/1‑97 (MQ=255)
                                                                                         gccgccAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGAt                                                                                     <  2:434939/97‑1 (MQ=255)
                                                                                         gccgccAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATcgccg                                           >  1:991274/1‑139 (MQ=255)
                                                                                               aGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAg                                     <  2:508381/139‑1 (MQ=255)
                                                                                                 cgcgGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTc                                   >  2:514426/1‑139 (MQ=255)
                                                                                                   cgGCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCa                                 <  2:654444/139‑1 (MQ=255)
                                                                                                    ggCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAg                                <  1:617225/139‑1 (MQ=255)
                                                                                                     gCATTATCCAGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAgg                               <  2:109658/139‑1 (MQ=255)
                                                                                                              aGACGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGt                      <  2:329111/139‑1 (MQ=255)
                                                                                                                aCGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAg                                                                                >  2:119614/1‑79 (MQ=255)
                                                                                                                aCGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAg                                                                                <  1:119614/79‑1 (MQ=255)
                                                                                                                aCGTTTGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg                    <  1:290647/139‑1 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATCATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTa               >  2:1102861/1‑139 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc                                        <  2:1071361/114‑1 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcgc                                        >  1:1071361/1‑114 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcg                                         <  1:1267907/113‑1 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCcgcg                                         >  2:1267907/1‑113 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg                    <  2:465083/134‑1 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTcg                    >  1:465083/1‑134 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTa               >  2:1254555/1‑139 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTa               >  1:1144584/1‑139 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTa               >  1:1131987/1‑139 (MQ=255)
                                                                                                                     tGCTTTCCATCGAGCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTa               >  2:924286/1‑139 (MQ=255)
                                                                                                                                  gCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCAcc  <  1:985345/139‑1 (MQ=255)
                                                                                                                                  gCTCCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCAcc  <  1:116551/139‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                       
GATGCATCGCGTCAAGCAAACGCCGTTTGGTTTCGGCGCTAATCGACTGCGGTTTACCGTGGGCATTGATGTAATTGGGGCTAATCCCCGCCGCCAGCGCGGCATTATCCAGACGTTTGCTTTCCATCGAGCTTCCTTAGCGTTTTGCCTGCCAGATACGAGCCTGATAATCGCGAATAGAGCGATCCGAGCTAAACATACCGCAGCGGGCGGTATTGAGGATCGCCGCGCGAGTCCAGGCCTCCTGGTCGCGGTACAGCACATCCACC  >  NZ_CP009273/3543303‑3543571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑