Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 3,562,031 C→A A220S (GCC→TCC)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,562,0310CA92.9% 201.5 / 0.3 56A220S (GCC→TCC) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base A (37/15);  total (39/17)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGG  >  NZ_CP009273/3561899‑3562165
                                                                                                                                    |                                                                                                                                      
cGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCaa                                                                                                                                   <  1:100830/138‑1 (MQ=255)
cGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCa                                                                                                                                    >  1:504993/1‑137 (MQ=255)
 gCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAg                                                                                                                                 <  2:306811/139‑1 (MQ=255)
      agagCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTa                                                                                                                            <  1:524530/139‑1 (MQ=255)
        agCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACg                                                                                                                              >  1:830569/1‑135 (MQ=255)
        agCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACg                                                                                                                              >  2:639055/1‑135 (MQ=255)
        agCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACg                                                                                                                              >  1:775532/1‑135 (MQ=255)
          cGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATa                                                                                                                        >  2:1065968/1‑139 (MQ=255)
          cGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATa                                                                                                                        >  2:912403/1‑139 (MQ=255)
                cGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATa                                                                                                                  <  2:516151/139‑1 (MQ=255)
                  ggTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACt                                                                                                                >  2:905464/1‑139 (MQ=255)
                  ggTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACt                                                                                                                >  2:627451/1‑139 (MQ=255)
                  ggTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACt                                                                                                                >  1:1173656/1‑139 (MQ=255)
                  ggTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGGTCTCATCGCGGTCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACt                                                                                                                >  1:913516/1‑139 (MQ=255)
                             gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGAtt                                                                                                     >  1:45291/1‑139 (MQ=255)
                             gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGAtt                                                                                                     >  2:143469/1‑139 (MQ=255)
                             gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGAtt                                                                                                     >  2:259633/1‑139 (MQ=255)
                             gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGAtt                                                                                                     >  1:170000/1‑139 (MQ=255)
                             gCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGAtt                                                                                                     >  2:288823/1‑139 (MQ=255)
                                     ggCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGa                                                                                             <  1:202323/139‑1 (MQ=255)
                                     ggCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGa                                                                                             <  2:535100/139‑1 (MQ=255)
                                     ggCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGa                                                                                             <  2:256387/139‑1 (MQ=255)
                                       cTTCGGTGATCTTGGGAATCAAATCTTTTCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATc                                                                                           >  2:989802/1‑139 (MQ=255)
                                       cTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATc                                                                                           >  2:935631/1‑139 (MQ=255)
                                       cTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATc                                                                                           >  1:1246530/1‑139 (MQ=255)
                                          cGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGt                                                                                         <  2:647405/138‑1 (MQ=255)
                                            gTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATAGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCg                                                                                      <  2:917591/139‑1 (MQ=255)
                                                 cTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCAtt                                                                                 >  1:243135/1‑139 (MQ=255)
                                                     ggAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAcg                                                                             >  2:1077009/1‑139 (MQ=255)
                                                     ggAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAc                                                                              <  1:143469/138‑1 (MQ=255)
                                                         tCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAcggcgg                                                                         >  2:304095/1‑139 (MQ=255)
                                                          cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAc                                                                              <  1:91564/133‑1 (MQ=255)
                                                          cAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGAc                                                                              >  2:91564/1‑133 (MQ=255)
                                                                 ttGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAg                                                                 >  1:759182/1‑139 (MQ=255)
                                                                  tGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCa                                                                  <  2:243135/137‑1 (MQ=255)
                                                                            gTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAAc                                                       >  2:394046/1‑138 (MQ=255)
                                                                                    aGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGa                                               >  1:19303/1‑138 (MQ=255)
                                                                                           aTCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTCTCAACGAATTCGATAATTTTa                                       <  2:795015/139‑1 (MQ=255)
                                                                                              gcgATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCg                                    >  2:817108/1‑139 (MQ=255)
                                                                                                gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGtt                                  >  2:964732/1‑139 (MQ=255)
                                                                                                gATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGtt                                  <  1:288823/139‑1 (MQ=255)
                                                                                                    gTCTTCTTCCAGCAGTTCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCtca                              <  1:1077009/139‑1 (MQ=255)
                                                                                                                   ttCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACg               >  2:295937/1‑139 (MQ=255)
                                                                                                                   ttCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGGTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACg               >  1:551909/1‑139 (MQ=255)
                                                                                                                    tCATACAGATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGc              >  2:1011778/1‑139 (MQ=255)
                                                                                                                           gATAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGc       >  1:1234518/1‑139 (MQ=255)
                                                                                                                            aTAGTCGGAGTCAAAGACGTAGATCCCCATCCTCGCCAGAGTTTTGCTCGGATCGTTCGTCATTGACGGCGGGTTAGCAGTTTTTCCAACGAATTCGATATTTTTTTCTTCCTCATCAACCGCCATAACGCCAAATGCg      <  2:45291/139‑1 (MQ=255)
                                                                                                                            aTAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGACATAACGCCAAATGCg      >  1:356887/1‑139 (MQ=255)
                                                                                                                            aTAGTCGCCGTAAAAGCCCTAGATCCCCATACCCGCCAGAAATTTGCTCGGATCGTTCGGCATTGACGGGGGGTTAGCAGTTTTTCCAACGAATTCAATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg      <  1:964732/139‑1 (MQ=255)
                                                                                                                             tAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACTCCAAATGCgg     >  1:528836/1‑139 (MQ=255)
                                                                                                                             tAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCgg     >  2:1160521/1‑139 (MQ=255)
                                                                                                                             tAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg      >  2:146700/1‑138 (MQ=255)
                                                                                                                             tAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg      >  1:648938/1‑138 (MQ=255)
                                                                                                                             tAGTCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCg      >  1:1200176/1‑138 (MQ=255)
                                                                                                                                tCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGNNNNg   >  2:412138/1‑138 (MQ=255)
                                                                                                                                tCGGAGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAgg  >  2:815487/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                                      
CGCAAGAGAGCGGGAACGGGTGCGCATAGGCCAGACCGGCTTCGGTGATCTTGGGAATCAAATCTTTGCCAAAGTCGTGGCTGGAGTTCTCATCGCGATCGTCTTCTTCCAGCAGTTCATACAGATAGTCGGCGTCAAAGACGTAGATACCCATACTCGCCAGAGATTTGCTCGGATCGTTCGGCATTGACGGCGGGTTAGCAGGTTTTTCAACGAATTCGATAATTTTATCGTTCTCATCAACCGCCATAACGCCAAATGCGGAGG  >  NZ_CP009273/3561899‑3562165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑